Information on EC 2.4.1.222 - O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

factor VII EGF repeat + UDP-D-GlcNAc

beta-D-glucosaminyl-factor VII EGF repeat + UDP

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659300

Substrates: Manic fringe modifies O-fucose on mouse Notch1 at extracellular epidermal growth factor-like repeats within the ligand-binding site and the Abruptex region expressed in Lec1 cells
Products: -

?

L-fucose + GlcNAc

GlcNAc-beta-1,3-fucitol

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441309

Substrates: -
Products: -

?

Notch + UDP-D-GlcNAc

beta-D-glucosaminyl-Notch + UDP

11 entries

Notch1 + UDP-D-GlcNAc

beta-D-glucosaminyl-Notch1 + UDP

O-fucose residues on Delta Notch ligand + UDP-GlcNAc

GlcNAc-beta-1,3-fucitol-Delta Notch

O-fucose residues on Jagged1 Notch ligand + UDP-GlcNAc

GlcNAc-beta1,3-fucitol-Jagged1 Notch

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Substrates: human Jagged1 ligand, exogenous expressed in Chinese hamster ovary Lec1 cells, experiment in vivo
Products: -

?

O-fucose residues on Serrate Notch ligand + UDP-GlcNAc

?

-

Substrates: in vitro
Products: -

?

O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc

GlcNAc-beta-1,3-fucitol-Notch

p-nitrophenyl-alpha-L-fucose + UDP-beta-D-GlcNAc

GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP

Substrates: -
Products: -

?

p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc

GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP

UDP-beta-D-GlcNAc + fucosyl-protein

UDP + O-beta-D-GlcNAc-fucosyl-protein

UDP-GlcNAc + Notch epidermal growth factor-like sequence repeat-O-fucose

UDP + ?

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759028

Substrates: the enzyme (Fringe) regulates fly Notch signaling by adding GlcNAc to O-fucose on Notch epidermal growth factor-like sequence repeats EGF8, -9, and -12
Products: -

?

[factor VII]-fucose + UDP-alpha-D-N-acetylglucosamine

[factor VII]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP

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Substrates: residues on EGF repeat from factor VII
Products: -

?

[factor VII]-fucose + UDP-alpha-N-acetyl-2-amino-2-deoxy-D-glucose

[factor VII]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP

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Substrates: residues on EGF repeat from factor VII
Products: -

?

[Notch]-fucose + UDP-alpha-D-N-acetylglucosamine

[Notch]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP

5 entries

additional information

?

-

37 entries

Notch + UDP-D-GlcNAc

beta-D-glucosaminyl-Notch + UDP

6 entries

UDP-GlcNAc + Notch epidermal growth factor-like sequence repeat-O-fucose

UDP + ?

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759028

Substrates: the enzyme (Fringe) regulates fly Notch signaling by adding GlcNAc to O-fucose on Notch epidermal growth factor-like sequence repeats EGF8, -9, and -12
Products: -

?

[Notch]-fucose + UDP-alpha-D-N-acetylglucosamine

[Notch]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP

4 entries

additional information

?

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Manganese

Mn2+

D-galactose

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Gal is required for the enhancement of Notch signaling by LFNG, whereas Gal inhibits the enhancement of DLL1-induced NOTCH signaling by MFNG

iroquois transcription factor

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Iro

691990

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UDP

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UMP

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additional information

-

enzyme expression is perturbed by injection of fringe morpholino antisense oligonucleotide resulting in a reduced number of secondary mesenchym cells and altered endoderm and mesoderm specification, as well as decreased and delayed archenteron invagination

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D-galactose

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Gal is required for the enhancement of Notch signaling by LFNG, whereas Gal inhibits the enhancement of DLL1-induced NOTCH signaling by MFNG

sloppy-paired transcription factor

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Slp

691990

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additional information

-

0.00116 - 0.129

p-nitrophenyl-alpha-L-fucose

15 entries

0.0343 - 0.0707

UDP-beta-D-GlcNAc

5 entries

0.08

UDP-beta-D-GlcNAc

wild-type, pH 6.8, 37°C

0.011

UDP

wild-type, pH 6.8, 37°C

0.096

UMP

wild-type, pH 6.8, 37°C

additional information

6.8

-

assay at

7

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assay at

659270

29

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enzyme assay

30

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assay at

37

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-

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673241, 673256, 759028

-

7 entries

sea urchin

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441308, 441310, 691961

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invaginated

brain

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in the developing brain, Lfng is expressed in self-renewing progenitors

manic fringe is highly expressed in claudin-low breast cancer

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expression is relatively constant during germ layer development and occurs in all cells, but becomes restricted during gastrulation

endoderm

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eye

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isoform lunatic fringe Lfng is expressed by progenitors in neurogenic regions and downregulated in cells that have initiated neuronal differentiation

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leg

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Lfng is expressed in the distal lung during saccular development

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prepolar cell

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during Drosophila oogenesis

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721917, 722798, 722885

T-lymphocyte

Rattus norvegicus

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expression levels of three Fringe homologues in naive CD4+T cells in asthmatic rats, overview. Radical Fringe (Rfng) is highly expressed, whereas both Lunatic Fringe (Lfng) and Manic Fringe (Mfng) are expressed at low levels

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691483, 691961

wing disc

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691483

additional information

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distribution, Fringe is expressed in both the signaling and the receiving cells during the first Delta-Notch signal

cytoplasmic MFNG is abundant in the normal kidney and renal carcinoma

fringe is a secreted protein

-

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apical and surface membranes

16020

microsome

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microsomal fraction enriched for Golgi membrane

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-

additional information

-

secreted protein

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Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

evolution

the enzyme belongs to the family of glycosyltransferases. Binding to UDP and presence of DxD motif are two significant characteristics of glycosyltransferases

malfunction

14 entries

metabolism

physiological function

18 entries

additional information

33000

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x * 53000, recombinant galectin-1-hum-MFng fusion protein, SDS-PAGE, x * 33000, recombinant detagged MFng, SDS-PAGE

34100

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predicted size of the fully processed, mature fragment

35000

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determined by SDS-PAGE and Western Blot analysis

43600

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predicted size of full-length unprocessed LFNG protein

53000

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x * 53000, recombinant galectin-1-hum-MFng fusion protein, SDS-PAGE, x * 33000, recombinant detagged MFng, SDS-PAGE

?

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x * 53000, recombinant galectin-1-hum-MFng fusion protein, SDS-PAGE, x * 33000, recombinant detagged MFng, SDS-PAGE

additional information

glycoprotein

presence of two N-linked glycans

671119

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crystal structure of mouse Manic Fringe bound to UDP and Mn2+

676137

sitting-drop vapour diffusion method. Abolishing the glycosylation sites by site-directed mutagenesis was necessary in order to obtain orthorhombic crystals that diffracted to 1.8 A resolution. Crystals belonged to space group P2(1)2(1)2. The unit-cell parameters (a = 162.6, b = 41.4, c = 38.8 A) are consistent with the presence of one molecule in the asymmetric unit

671119

D327N

EMS mutagenesis, transheterozygote, mutation on allele 14, lethal phenotype

E220K

EMS mutagenesis, transheterozygote, mutation on allele 3, lethal phenotype

G295D

EMS mutagenesis, transheterozygote, mutation on allele L81, lethal phenotype

K196E

EMS mutagenesis, transheterozygote, mutation on allele 11, lethal phenotype

K196N

EMS mutagenesis, transheterozygote, mutation on allele 11, lethal phenotype

L213F

EMS mutagenesis, transheterozygote, mutation on allele L83, lethal phenotype

L398Q

EMS mutagenesis, transheterozygote, mutation on allele 2, phenotype overview

S350T

EMS mutagenesis, transheterozygote, mutation on allele 1, lethal phenotype

T184M

EMS mutagenesis, transheterozygote, phenotype overview

G256S

Q8NES3

mutation identified in a patient with SCD3 phenotype and scoliosis. Variant is correctly localized and processed, but is nonfunctional

777588

R174H

Q8NES3

mutation identified in a patient with SCD3 phenotype and scoliosis. Variant R174H is correctly localized and processed, but is functionally less effective than wild-type

A175V

short loop mutant

A235Y

long loop mutant

C290S

active site mutant to short loop side of residue D289

D288A

active site mutant to short loop side of residue D289, activity too low to measure

D289E

active site mutant, catalytically inactive

E237A

long loop mutant

F251S

active site mutant, catalytically inactive

F251Y

active site mutant to short loop side of residue D289

G254A

active site mutant to short loop side of residue D289

G334H

donor specificity mutant, 94.6% of wild-type UDP-beta-D-GlcNAc utilization, 78.0% of wild-type UDP-Glc utilization

H171A

short loop mutant

H171D

short loop mutant

H313A

donor specificity mutant, 6.2% of wild-type UDP-beta-D-GlcNAc utilization, 75.9% of wild-type UDP-Glc utilization

H313A/G334H

donor specificity mutant, 3.1% of wild-type UDP-beta-D-GlcNAc utilization, 34.7% of wild-type UDP-Glc utilization

I233A

long loop mutant

L176A

short loop mutant

L229Q

long loop mutant

L314R

donor specificity mutant, 21.7% of wild-type UDP-beta-D-GlcNAc utilization, 119.5% of wild-type UDP-Glc utilization

S128V

short loop mutant

S168A

short loop mutant

S228A

active site mutant to short loop side of residue D289

S228L

active site mutant, catalytically inactive

s228T

active site mutant to short loop side of residue D289

S228Y

active site mutant, catalytically inactive

S312T

donor specificity mutant, 1.9% of wild-type UDP-beta-D-GlcNAc utilization, 18.1% of wild-type UDP-Glc utilization

T253A

active site mutant, catalytically inactive

additional information

8 entries

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on a His-Select nickel affinity gel

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recombinant enzyme

recombinant fusion protein galectin-1-hum-MFng from Escherichia coli strain Rosetta (DE3) pLysS, the fusion protein is cleaved with Tev protease to release the MFng protein, further purification by UDP-agarose chromatography

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recombinant His-tagged enzyme by nickel affinity chromatography

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catalytic domain of murine Manic Fringe is expressed in the baculovirus/insect-cell system as a secreted protein

671119

coexpression of Flag-tagged DLL3 and HA-tagged LFNG in CHO cells, the two proteins coprecipitate

O09010

737075

DNA acid sequence determination and analysis, expression as insoluble His-tagged protein in Escherichia coli strain XL1-Blue

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expressed in Drosophila S2 cells

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441308, 441310

expressed in HEK-293T cell

O09008, O09010, O09009

778023

expression of MFeng as soluble protein in fusion with galectin-1 from pLgals1-hum-MFng in Escherichia coli Rosetta (DE3) pLysS

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expression of Radical Fringe, Lunatic Fringe, and Manic Fringe in HEK 293T cells and NIH 3T3 cells

Rattus norvegicus

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659910

full-length, His-tagged FNG is expressed in S2 cells from a pMTHy vector construct

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693015

gene fng, DNA and amino acid sequence determination and analysis

gene Lfng, Lfng is located at 7p22 arm of human chromosome

gene LFNG, quantitative RT-PCR enzyme expression analysis

gene Mfng, Mfng maps to human chromosome 22q13.1

gene Rfng, Rfng is localized at 17q25 arm of human chromosome

genes lfng and mfng, expression in wild-type CHO cells, in Lec1.3C, Lec2.6A, Lec8.3D, and Lec20.15C CHO glycosylation mutant lines, and in Lec8 and Lec20 CHO mutant lines

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Lfng overexpression in transgenic mouse model

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722885

Lunatic Fringe and Manic Fringe expressed in Chinese hamster ovary Lec1 cells

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441308

Manic Fringe stable expressed in Chinese hamster ovary Lec1 cells

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mouse FRINGE coding sequences are ligated into pcDNA3 for transfection of NIH3T3 cells

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Notch alone or Fringe alone expressed in Drosophila SL2 cells or Notch and Fringe co-expressed in Drosophila SL2 cells

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441309

overexpression in S2 cells

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659270

MFNG gene expression is upregulated in kidney clear cell renal carcinoma (KIRC) patients. There was no significant association between the patient survival probability and the level of MFNG expression in this cohort