microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes. The enzyme interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate
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the enzyme (PtUGT1) is cocrystallized with indoxyl sulfate in sitting drops consisting of 0.0002 ml protein solution and 0.0002 ml crystallization buffer. Crystal structure is solved at 2.14 A resolution with indoxyl sulfate, a glycosylation-incompetent indoxyl analog, in the active site