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Information on EC 2.4.1.220 - indoxyl-UDPG glucosyltransferase
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2.4.1.220 indoxyl-UDPG glucosyltransferaseIUBMB Comments
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
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The enzyme appears in viruses and cellular organisms
Synonyms
hydroxyindole glucosyltransferase, indican synthase, indoxyl glucosyltransferase, indoxyl-UDPG-glucolsyltransferase, PtIGS, PtUGT1, PtUGT2, UDP-glucose:indoxyl glucosyltransferase, UGIG, UGT72B29, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
hydroxyindole glucosyltransferase
-
-
-
-
indican synthase
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-
-
-
indoxyl glucosyltransferase
-
-
-
-
indoxyl-UDPG-glucolsyltransferase
-
-
-
-
PtUGT2
-
differs from PtUGT1 by four amino acid residues V19M, G225A, E230Q, and A423D
UDP-glucose:indoxyl glucosyltransferase
UDP-glucose:indoxyl glucosyltransferase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + indoxyl = UDP + indican
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycosyl group transfer
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-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:indoxyl 3-O-beta-D-glucosyltransferase
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
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CAS REGISTRY NUMBER
COMMENTARY
258339-72-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 4-hydroxyindole
UDP + 1H-indol-4-yl-beta-D-glucopyranoside
-
minimal activity
-
-
?
UDP-glucose + 5-hydroxyindole
UDP + 1H-indol-5-yl-beta-D-glucopyranoside
-
activity as 3-OH-indole
-
-
?
UDP-glucose + 6-hydroxyindole
UDP + 1H-indol-6-yl-beta-D-glucopyranoside
-
minimal activity
-
-
?
UDP-glucose + 7-hydroxyindole
UDP + 1H-indol-7-yl-beta-D-glucopyranoside
-
-
-
-
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
best substrate
i.e. indican
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
best substrate
i.e. indican
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
i.e. indole-3-ol or 3-OH-indole
i.e. indican
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
i.e. indican
?
UDP-glucose + indoxyl
UDP + indican
the enzyme functions as an indican synthase in plant cells
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
i.e. indican
?
UDP-glucose + indoxyl
UDP + indican
the enzyme functions as an indican synthase in plant cells
-
-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
in expression analyses PtIGS mRNA is found only in the leaves. It is most highly expressed in the 1st leaves, and decreased with leaf age
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes. The enzyme interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate
brenda
microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes
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brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the enzyme (PtUGT1) is cocrystallized with indoxyl sulfate in sitting drops consisting of 0.0002 ml protein solution and 0.0002 ml crystallization buffer. Crystal structure is solved at 2.14 A resolution with indoxyl sulfate, a glycosylation-incompetent indoxyl analog, in the active site
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
anaerobic conditions, since under aerobic conditions indoxyl dimerizes spontaneously to indigo
-
488949
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marcinek, H.; Weyler, W.; Deus-Neumann, B.; Zenk, M.H.
Indoxyl-UDPG-glucosyltransferase from Baphicacanthus cusia
Phytochemistry
53
201-207
2000
Strobilanthes cusia
brenda
Minami, Y.; Nishimura, O.; Hara-Nishimura, I.; Nishimura, M.; Matsubara, H.
Tissue and intracellular localization of indican and the purification and characterization of indican synthase from indigo plants
Plant Cell Physiol.
41
218-225
2000
Persicaria tinctoria
brenda
Hsu, T.M.; Welner, D.H.; Russ, Z.N.; Cervantes, B.; Prathuri, R.L.; Adams, P.D.; Dueber, J.E.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy
Nat. Chem. Biol.
14
256-261
2018
Persicaria tinctoria, Persicaria tinctoria (A0A2R2JFJ4)
brenda
Inoue, S.; Morita, R.; Kuwata, K.; Kunieda, T.; Ueda, H.; Hara-Nishimura, I.; Minami, Y.
Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium
Plant Physiol. Biochem.
132
138-144
2018
Persicaria tinctoria (A0A2L2R220), Persicaria tinctoria
brenda
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