Information on EC 2.4.1.220 - indoxyl-UDPG glucosyltransferase

for references in articles please use BRENDA:EC2.4.1.220
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.4.1.220
-
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RECOMMENDED NAME
GeneOntology No.
indoxyl-UDPG glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + indoxyl = UDP + indican
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indican biosynthesis
-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:indoxyl 3-O-beta-D-glucosyltransferase
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
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CAS REGISTRY NUMBER
COMMENTARY hide
258339-72-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
indigo plant
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-glucose + indoxyl
ADP + indican
show the reaction diagram
-
-
-
?
GDP-glucose + indoxyl
GDP + indican
show the reaction diagram
-
-
-
?
UDP-glucose + 4-hydroxyindole
UDP + 1H-indol-4-yl-beta-D-glucopyranoside
show the reaction diagram
-
minimal activity
-
-
?
UDP-glucose + 5-hydroxyindole
UDP + 1H-indol-5-yl-beta-D-glucopyranoside
show the reaction diagram
-
activity as 3-OH-indole
-
-
?
UDP-glucose + 6-hydroxyindole
UDP + 1H-indol-6-yl-beta-D-glucopyranoside
show the reaction diagram
-
minimal activity
-
-
?
UDP-glucose + 7-hydroxyindole
UDP + 1H-indol-7-yl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
show the reaction diagram
additional information
?
-
-
not: 2-OH-indole
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
show the reaction diagram
-
indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
i.e. indican
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
4-hydroxyindole
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-
0.5
5-hydroxyindole
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-
3.3
6-hydroxyindole
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-
1.4
7-hydroxyindole
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-
1.2
indoxyl
-
-
0.13 - 1.7
UDP-glucose
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
pI: 6.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 10.2
-
6.8 and 10.2 half-maximal-activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
30 and 50 half-maximal-activity
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
gel-filtration
53000
-
SDS-PAGE
60000
-
SDS-PAGE
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
anaerobic conditions, since under aerobic conditions indoxyl dimerizes spontaneously to indigo
-
488949
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.220)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)