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IUBMB Comments Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
The enzyme appears in viruses and cellular organisms
Synonyms
hydroxyindole glucosyltransferase, indican synthase, indoxyl glucosyltransferase, indoxyl-UDPG-glucolsyltransferase, PtIGS, PtUGT1, PtUGT2, UDP-glucose:indoxyl glucosyltransferase, UGIG, UGT72B29,
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hydroxyindole glucosyltransferase
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indoxyl glucosyltransferase
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indoxyl-UDPG-glucolsyltransferase
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PtUGT1
predominant isoform
PtUGT2
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differs from PtUGT1 by four amino acid residues V19M, G225A, E230Q, and A423D
UDP-glucose:indoxyl glucosyltransferase
UDP-glucose:indoxyl glucosyltransferase
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UDP-glucose:indoxyl glucosyltransferase
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UDP-glucose + indoxyl = UDP + indican
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glycosyl group transfer
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UDP-glucose:indoxyl 3-O-beta-D-glucosyltransferase
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
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ADP-glucose + indoxyl
ADP + indican
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?
GDP-glucose + indoxyl
GDP + indican
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?
UDP-glucose + 4-hydroxyindole
UDP + 1H-indol-4-yl-beta-D-glucopyranoside
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minimal activity
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?
UDP-glucose + 5-hydroxyindole
UDP + 1H-indol-5-yl-beta-D-glucopyranoside
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activity as 3-OH-indole
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?
UDP-glucose + 6-hydroxyindole
UDP + 1H-indol-6-yl-beta-D-glucopyranoside
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minimal activity
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?
UDP-glucose + 7-hydroxyindole
UDP + 1H-indol-7-yl-beta-D-glucopyranoside
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?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
UDP-glucose + indoxyl
UDP + indican
additional information
?
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not: 2-OH-indole
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?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
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best substrate
i.e. indican
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UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
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best substrate
i.e. indican
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UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
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i.e. indole-3-ol or 3-OH-indole
i.e. indican
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UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
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indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
i.e. indican
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UDP-glucose + indoxyl
UDP + indican
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UDP-glucose + indoxyl
UDP + indican
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UDP-glucose + indoxyl
UDP + indican
the enzyme functions as an indican synthase in plant cells
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?
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UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
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indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
i.e. indican
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UDP-glucose + indoxyl
UDP + indican
the enzyme functions as an indican synthase in plant cells
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?
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0.13
UDP-glucose
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0.74
UDP-glucose
pH 10.0, 37ưC
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additional information
additional information
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9.1
UDP-glucose
pH 7.8, 30ưC
12
UDP-glucose
pH 7.8, 30ưC
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additional information
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10
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6.8 - 10.2
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6.8 and 10.2 half-maximal-activity
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30 - 50
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30 and 50 half-maximal-activity
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5.39
calculated from sequence
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brenda
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UniProt
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UniProt
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indigo plant
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in expression analyses PtIGS mRNA is found only in the leaves. It is most highly expressed in the 1st leaves, and decreased with leaf age
brenda
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microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes. The enzyme interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate
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microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes
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A0A2L2R220_9CARY
477
0
51913
TrEMBL
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A0A2R2JFJ4_9CARY
478
0
51850
TrEMBL
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51911
calculated from sequence
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the enzyme (PtUGT1) is cocrystallized with indoxyl sulfate in sitting drops consisting of 0.0002 ml protein solution and 0.0002 ml crystallization buffer. Crystal structure is solved at 2.14 A resolution with indoxyl sulfate, a glycosylation-incompetent indoxyl analog, in the active site
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anaerobic conditions, since under aerobic conditions indoxyl dimerizes spontaneously to indigo
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488949
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expression in Escherichia coli
heterologous expression of PtUGT1 in Escherichia coli
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Marcinek, H.; Weyler, W.; Deus-Neumann, B.; Zenk, M.H.
Indoxyl-UDPG-glucosyltransferase from Baphicacanthus cusia
Phytochemistry
53
201-207
2000
Strobilanthes cusia
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Minami, Y.; Nishimura, O.; Hara-Nishimura, I.; Nishimura, M.; Matsubara, H.
Tissue and intracellular localization of indican and the purification and characterization of indican synthase from indigo plants
Plant Cell Physiol.
41
218-225
2000
Persicaria tinctoria
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Hsu, T.M.; Welner, D.H.; Russ, Z.N.; Cervantes, B.; Prathuri, R.L.; Adams, P.D.; Dueber, J.E.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy
Nat. Chem. Biol.
14
256-261
2018
Persicaria tinctoria, Persicaria tinctoria (A0A2R2JFJ4)
brenda
Inoue, S.; Morita, R.; Kuwata, K.; Kunieda, T.; Ueda, H.; Hara-Nishimura, I.; Minami, Y.
Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium
Plant Physiol. Biochem.
132
138-144
2018
Persicaria tinctoria (A0A2L2R220), Persicaria tinctoria
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