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Information on EC 2.4.1.211 - 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase and Organism(s) Lachnoclostridium phytofermentans and UniProt Accession A9KQ75

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IUBMB Comments
Reaction also occurs with beta-D-galactopyranosyl-(1->3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product.
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This record set is specific for:
Lachnoclostridium phytofermentans
UNIPROT: A9KQ75
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The taxonomic range for the selected organisms is: Lachnoclostridium phytofermentans
Synonyms
1,3-beta-galactosyl-N-acetylhexosamine phosphorylase, beta-1,3-galactosyl-N-acetylhexosamine phosphorylase, beta-D-galactopyranosyl-(1-3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase, cphy0577 protein, cphy3030 protein, D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase, Gal-GlyNAcP, Gal-HexNAcP, galacto-N-biose phosphorylase, galacto-N-biose/lacto-N-biose I phosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-D-galactopyranosyl-(1-3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase
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cphy0577 protein
285799
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cphy3030 protein
285800
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D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase
285799, 285800
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GalHexNAcP
285799, 285800
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GNB/LNB phosphorylase
285799, 285800
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase
Reaction also occurs with beta-D-galactopyranosyl-(1->3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product.
CAS REGISTRY NUMBER
COMMENTARY hide
224427-06-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
galacto-N-biose + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
r
lacto-N-biose I + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
galacto-N-biose + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
r
lacto-N-biose I + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
show the reaction diagram
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-
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r
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.3
N-acetyl-D-galactosamine
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1.9
N-acetyl-D-glucosamine
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3.3
N-acetyl-D-galactosamine
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3.4
N-acetyl-D-glucosamine
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
34
N-acetyl-D-galactosamine
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48
N-acetyl-D-galactosamine
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
optimum activity at neutral pH
6 - 7
optimum activity at neutral pH
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bacterium possesses three GalHexNAcP homologous genes, Cphy3030 gene; anaerobic cellulolytic bacterium
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
proteins are predicted not to possess N-terminal signal peptides
Manually annotated by BRENDA team
proteins are predicted not to possess N-terminal signal peptides
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
protein is thermostable up to 30°C
45
protein is thermostable up to 45°C
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
suitable catalyst for practical syntheses of oligosaccharides
synthesis
suitable catalyst for practical syntheses of oligosaccharides
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakajima, M.; Nishimoto, M.; Kitaoka, M.
Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylase
J. Biol. Chem.
284
19220-19227
2009
Lachnoclostridium phytofermentans (A9KIW5), Lachnoclostridium phytofermentans (A9KQ75), Lachnoclostridium phytofermentans
Manually annotated by BRENDA team
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