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Information on EC 2.4.1.206 - lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase and Organism(s) Mus musculus and UniProt Accession Q8BGY6
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2.4.1.206 lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferaseSpecify your search results
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
glcnac transferase, beta3gn-t5, beta3gn-t2, lc3 synthase, beta3gnt5, lactosylceramide n-acetylglucosaminyltransferase, lc3 synthase/bgn-t5, beta1-3-n-acetylglucosaminyltransferase, la2 synthase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase
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acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-lactosylceramide beta-
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beta1-3-N-acetylglucosaminyltransferase
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LA2 synthase
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lactosylceramide beta-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-lactosylceramide beta-acetylglucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3-beta-N-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
83682-80-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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i.e. lactosylceramide
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?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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glycolipid biosynthesis
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UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
best substrate
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
acceptor substrate specificity, overview
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additional information
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the enzyme synthesizes the key intermediate lactosyltriosylceramide for lacto- and neolactoglycolipids. The large increase in the activity of Lc3 synthase appears to regulate the levels of other neolacto glycolipids, such as Lc3cer, nLc4Cer, nLc6Cer, disialosyl-nLc4Cer and Le(x)-glycolipids
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additional information
?
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Lc3 synthase utilizes a variety of galactose-terminated glycolipids as acceptors by establishing a glycosidic bond in the beta-1,3-linkage to GlcNaAc to extend the lacto- and neolacto-series gangliosides
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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glycolipid biosynthesis
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?
additional information
?
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the enzyme synthesizes the key intermediate lactosyltriosylceramide for lacto- and neolactoglycolipids. The large increase in the activity of Lc3 synthase appears to regulate the levels of other neolacto glycolipids, such as Lc3cer, nLc4Cer, nLc6Cer, disialosyl-nLc4Cer and Le(x)-glycolipids
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?
additional information
?
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Lc3 synthase utilizes a variety of galactose-terminated glycolipids as acceptors by establishing a glycosidic bond in the beta-1,3-linkage to GlcNaAc to extend the lacto- and neolacto-series gangliosides
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?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00123
recombinant enzyme, substrate lD-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
0.00174
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.0021
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.00254
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
distinct cellular layers: Purkinje cell layer, molecular cell layer, granule cell layer + white matter
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E17 fetus, both head and body show strong expression of Lc3 synthase in wild-type (Wt/Wt) and heterozygous (Wt/KO) mice
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
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Lc3 synthase is a member of the beta-1,3-N-glycosyltransferase superfamily
malfunction
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multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase, detailed overview. B3gnt5 gene knockout mutant mice appear normal in the embryonic stage and, if they survive delivery, remain normal during early life, but about 9% develop early-stage growth retardation, 11% die postnatally in less than 2 months, and adults tend to die in 5-15 months, demonstrating splenomegaly and notably enlarged lymph nodes. Without lacto-neolacto series gangliosides, both homozygous and heterozygous mice gradually display fur loss or obesity, and breeding mice demonstrate reproductive defects. B3gnt5 gene knockout disrupts the functional integrity of B cells with a decrease in B-cell numbers in the spleen, germinal center disappearance, and less efficiency to proliferate in hybridoma fusion
physiological function
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Lc3 synthase is a key enzyme in lacto-neolacto ganglioside synthesis, it exhibits strong activity in transferring GlcNAc to the lactosylceramide for lacto-neolacto-chain ganglioside biosynthesis, regulated during embryonic development, especially during brain morphogenesis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). B3GN5_MOUSE
376
1
43914
Swiss-Prot
Secretory Pathway (Reliability: 4)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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generation of Lc3 synthase gene B3gnt5-deficient knockout mice, phenotypes, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chou, D.K.H.; Jungalwala, F.B.
N-acetylglucosaminyl Transferase regulates the expression of sulfoglucuronyl glycolipids in specific cell types in cerebellum during development
J. Biol. Chem.
271
28868-28874
1996
Mus musculus, Rattus norvegicus
Henion, T.R.; Zhou, D.; Wolfer, D.P.; Jungalwala, F.B.; Hennet, T.
Cloning of a mouse beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis
J. Biol. Chem.
276
30261-30269
2001
Homo sapiens (Q9BYG0), Mus musculus (Q8BGY6), Mus musculus, no activity in Spodoptera frugiperda
Chou, D.K.H.; Henion, T.R.; Jungalwala, F.B.
Regulation of expression of sulfoglucuronyl carbohydrate (HNK-1), amphoterin and RAGE in retinoic acid-differentiated P19 embryonal carcinoma cells
J. Neurochem.
86
917-931
2003
Mus musculus
Kuan, C.T.; Chang, J.; Mansson, J.E.; Li, J.; Pegram, C.; Fredman, P.; McLendon, R.E.; Bigner, D.D.
Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase - a key enzyme in lacto-neolacto ganglioside synthesis
BMC Dev. Biol.
10
114
2010
Mus musculus, Mus musculus C57BL/6
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