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Information on EC 2.4.1.203 - trans-zeatin O-beta-D-glucosyltransferase and Organism(s) Phaseolus lunatus and UniProt Accession Q9ZSK5

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.203 trans-zeatin O-beta-D-glucosyltransferase
IUBMB Comments
Unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-beta-D-xylosyltransferase).
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This record set is specific for:
Phaseolus lunatus
UNIPROT: Q9ZSK5
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The taxonomic range for the selected organisms is: Phaseolus lunatus
The enzyme appears in selected viruses and cellular organisms
Synonyms
ugt76c2, ugt73c5, ugt76c1, ugt85a1, trans-zeatin o-glucosyltransferase, ugt73c1, glucosyltransferase ugt85a1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trans-zeatin O-beta-D-glucosyltransferase
-
zeatin O-glucosyltransferase
-
glucosyltransferase, uridine diphosphoglucose-zeatin O-
-
-
-
-
trans-zeatin O-glucosyltransferase
-
-
uridine diphosphoglucose-zeatin O-glucosyltransferase
-
-
-
-
zeatin glycosyltransferase
GT
zeatin O-beta-D-glucosyltransferase
-
-
-
-
zeatin O-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + trans-zeatin = UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
localisation of site determining the substrate specificity for the UDP-sugar donors
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-zeatin O-beta-D-glucosyltransferase
Unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-beta-D-xylosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
123644-76-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose + cis-zeatin
UDP + O-beta-D-glucosyl-cis-zeatin
show the reaction diagram
-
-
-
?
UDP-galactose + trans-zeatin
UDP + O-galactosylzeatin
show the reaction diagram
-
-
?
UDP-glucose + m-topolin
UDP + O-beta-D-glucosyl-m-topolin
show the reaction diagram
23% of the activity with trans-zeatin
-
-
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
-
-
?
UDP-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
show the reaction diagram
-
-
?
UDP-xylose + trans-zeatin
UDP + O-xylosylzeatin
show the reaction diagram
-
-
?
UDP-galactose + trans-zeatin
UDP + O-galactosylzeatin
show the reaction diagram
-
-
-
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
-
important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
-
-
?
UDP-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
show the reaction diagram
-
converts dihydrozeatin exclusively with UDP-D-xylose as donor substrates
-
?
UDP-xylose + trans-zeatin
UDP + O-xylosylzeatin
show the reaction diagram
-
-
-
?
additional information
?
-
no activity with o-hydroxythidiazuron
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
-
-
?
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
-
important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
cis-zeatin
-
0.14
m-topolin
-
0.05
trans-zeatin
-
0.028
trans-zeatin
-
-
0.216
UDP-glucose
-
-
2.7
UDP-xylose
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
cis-zeatin
-
0.08
m-topolin
-
0.45
trans-zeatin
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00001166
F149L mutant
0.00001666
F149Y mutant
0.0000216
R59K mutant
0.0000233
L127M mutant
0.0000316
D87N mutant
0.0000533
D87A mutant
0.0000916
D87E mutant
0.000105
L58A mutant
0.00015
R54A mutant
0.000175
D86A mutant
0.000186
E85A mutant
0.0002
wild type
0.000205
Q55A mutant
0.57
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immature
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ZOG_PHALU
459
0
51412
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
gel filtration
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D86A
site-directed mutagenesis
D87A
site-directed mutagenesis
D87E
site-directed mutagenesis
D87N
site-directed mutagenesis
E85A
site-directed mutagenesis
F149A
site-directed mutagenesis
F149L
site-directed mutagenesis
F149Y
site-directed mutagenesis
L127A
site-directed mutagenesis, no activity
L127F
site-directed mutagenesis, no activity
L127M
site-directed mutagenesis
L58A
site-directed mutagenesis
Q55A
site-directed mutagenesis
R54A
site-directed mutagenesis
R59A
site-directed mutagenesis, no activity
R59K
site-directed mutagenesis
R59Q
site-directed mutagenesis, no activity
additional information
-
construction of chimeric recombinant enzyme mutants, exchange of the C-terminus with zeatin O-beta-D-xylosyltransferase, EC 2.4.2.40, gene ZOX1, determination of the site determining the substrate specificity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, expression in Escherichia coli BL21(DE3) cells
expressed in Nicotiana tabacum cultivar Wisconsin 38 leaves
-
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dixon, S.C.; Martin, R.C.; Mok, M.C.; Shaw, G.; Mok, D.W.S.
Zeatin glycosylation enzymes in Phaseolus - isolation of O-glucosyltransferase from Phaseolus lunatus and comparison to O-xylosyltransferase from P. vulgaris
Plant Physiol.
90
1316-1321
1989
no activity in Phaseolus vulgaris, Phaseolus lunatus
Manually annotated by BRENDA team
Martin, R.C.; Mok, M.C.; Mok, D.W.S.
Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus
Proc. Natl. Acad. Sci. USA
96
284-289
1999
Phaseolus lunatus (Q9ZSK5), Phaseolus lunatus
Manually annotated by BRENDA team
Martin, R.C.; Cloud, K.A.; Mok, M.C.; Mok, D.W.S.
Substrate specificity and domain analyses of zeatin O-glycosyltransferases
Plant Growth Regul.
32
289-293
2000
Phaseolus lunatus, Zea mays
-
Manually annotated by BRENDA team
Mok, M.C.; Martin, R.C.; Dobrev, P.I.; Vankova, R.; Ho, P.S.; Yonekura-Sakakibara, K.; Sakakibara, H.; Mok, D.W.
Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition
Plant Physiol.
137
1057-1066
2005
Phaseolus lunatus (Q9ZSK5)
Manually annotated by BRENDA team
Polanska, L.; Vicankova, A.; Novakova, M.; Malbeck, J.; Dobrev, P.I.; Brzobohaty, B.; Vankova, R.; Machackova, I.
Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco
J. Exp. Bot.
58
637-649
2007
Phaseolus lunatus
Manually annotated by BRENDA team
Rodo, A.P.; Brugiere, N.; Vankova, R.; Malbeck, J.; Olson, J.M.; Haines, S.C.; Martin, R.C.; Habben, J.E.; Mok, D.W.; Mok, M.C.
Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation
J. Exp. Bot.
59
2673-2686
2008
Phaseolus lunatus (Q9ZSK5), Zea mays
Manually annotated by BRENDA team
Meek, L.; Martin, R.C.; Shan, X.; Karplus, P.A.; Mok, D.W.; Mok, M.C.
Isolation of Legume Glycosyltransferases and Active Site Mapping of the Phaseolus lunatus Zeatin O-glucosyltransferase ZOG1
J. Plant Growth Regul.
27
192-201
2008
Glycine max (Q8S3B9), Phaseolus lunatus (Q8S3B6), Phaseolus vulgaris (A7L745)
-
Manually annotated by BRENDA team
Haisel, D.; Vankova, R.; Synkova, H.; Pospisilova, J.
The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange
Biol. Plant.
52
49-58
2008
Phaseolus lunatus
-
Manually annotated by BRENDA team