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Information on EC 2.4.1.201 - alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
for references in articles please use BRENDA:EC2.4.1.201
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.201 alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
IUBMB Comments
Requires a high concentration of Mn2+ for maximal activity. The enzyme, characterized from hen oviduct membranes, participates in the processing of N-glycans in the Golgi apparatus. It transfers GlcNAc in β1-4 linkage to a D-mannose residue that already has GlcNAc residues attached at positions 2 and 6 by β linkages. No homologous enzyme appears to exist in mammals.
The enzyme appears in viruses and cellular organisms
- 2.4.1.201
- 5-phosphate
- 1-deoxy-d-xylulose
- fosmidomycin
-
isoprenoids
- valine
-
falciparum
- methylerythritol
-
reductoisomerase
- isoleucine
-
n-glycans
-
1-deoxyxylulose
-
mtdna
Reaction Schemes
show+
=
+
+
=
+
Synonyms
gnt vi, mgat6, n-acetylglucosaminyltransferase vi, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta-1,4-, VI
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acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta-1-4-, VI
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GnT VI
mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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N-acetylglucosaminyltransferase VI
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase VI
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UDP-GlcNAc: GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man] beta1-4N-acetylglucosaminyltransferase VI
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UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI
UDP-N-acetyl-D-glucosamine (GlcNAc):GlcNAcbeta1-6(GlcNAcbeta1-2)-Manalpha1-R[GlcNAc to Man]beta1,4N-acetylglucosaminyltransferase VI
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uridine diphosphoacetylglucosamine-glycopeptide beta-1-4-acetylglucosaminyltransferase VI
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N-acetylglucosaminyltransferase VI
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UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
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UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
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UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
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UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI
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UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->2)]-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Requires a high concentration of Mn2+ for maximal activity. The enzyme, characterized from hen oviduct membranes, participates in the processing of N-glycans in the Golgi apparatus. It transfers GlcNAc in beta1-4 linkage to a D-mannose residue that already has GlcNAc residues attached at positions 2 and 6 by beta linkages. No homologous enzyme appears to exist in mammals.
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CAS REGISTRY NUMBER
COMMENTARY
119699-68-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-octyl
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-glucosyl-octyl
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Substrates: synthetic acceptor substrate
Products: identification and large scale preparation of the product
Products: identification and large scale preparation of the product
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-(CH2)8-CH3
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-(CH2)8-CH3
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Substrates: synthetic acceptor substrate
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-(CH2)8-COOH
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-(CH2)8-COOH
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Substrates: synthetic acceptor substrate
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-pyridylamine
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-pyridylamine
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Substrates: fluorescent synthetic substrate
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: acceptor substrate specificity
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: R represents the remainder of the N-oligosaccharide core of asparagine-linked glycopeptides, (+/-)-fucose, minimum structure requirement: N-acetyl-D-glucosaminyl-beta-1,6-(N-acetylglucosaminyl-beta-1,2)-alpha-mannosyl-R
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
Substrates: R represents the remainder of the N-oligosaccharide core of asparagine-linked glycopeptides, (+/-)-fucose, minimum structure requirement: N-acetyl-D-glucosaminyl-beta-1,6-(N-acetylglucosaminyl-beta-1,2)-alpha-mannosyl-R
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: biantennary oligosaccharides, i.e. Gn T I and II reaction products, are no substrates
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: acts on bisected and non-bisected substrates
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: compounds lacking beta-2-linked N-acetylglucosamine residues are no acceptor substrates
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: reaction in biosynthesis of penta-antennary oligosaccharides of hen ovomucoid
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: involved in N-glycan biosynthesis
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: biosynthesis of pentaantennary asparagine-linked N-glycans
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
Substrates: biosynthesis of pentaantennary asparagine-linked N-glycans
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
additional information
?
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Substrates: only asialo-agalacto glycoproteins with GlcNAcbeta1-6Manalpha1 branches are able to serve as substrate
Products: -
Products: -
?
additional information
?
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Substrates: evaluation of a highly sensitive method for determination of the activity of GnT VI using a fl uorescently labeled tetraantennary oligosaccharide as an acceptor substrate. GnT VI stringently requires beta1,6-N-acetylglucosaminylation on the Manalpha1-6 arm (GnT V product, EC 2.4.1.155) as an acceptor substrate. Agalacto-triantennary oligosaccharide (GnT V product) can also be used as an alternate substrate
Products: -
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: reaction in biosynthesis of penta-antennary oligosaccharides of hen ovomucoid
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: involved in N-glycan biosynthesis
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: biosynthesis of pentaantennary asparagine-linked N-glycans
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
Substrates: biosynthesis of pentaantennary asparagine-linked N-glycans
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
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Substrates: -
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Mg2+
Mn2+
Ni2+
additional information
additional information
-
requirement for divalent cations, no effect of Zn2+, Fe2+, Cu2+
additional information
-
divalent cation-dependent enzyme activity, maximal activity with Mn2+. Co2+ and Mg 2+ can partially substitute for Mn2+
additional information
-
absolute requirement for divalent cations, Cu2+ cannot substitute Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.09
N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8.4
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about half-maximal activity at pH 5.0 and about 80% of maximal activity at pH 8.4
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
-
the enzyme contributes to the surface expression of CD133 through the processing of multiantennary N -linked glycans on CD133
metabolism
-
six different N-acetylglucosaminyltransferases (GnTs) I through VI are involved in the GlcNAc branching of the core
metabolism
-
the enzyme is involved in the N-glycan-branching pathway, regulation of cellular metabolite levels by Mgat6, overview
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60000
additional information
additional information
type II transmembrane protein, primary structure, amino acid sequence
additional information
-
type II transmembrane protein, primary structure, amino acid sequence
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
GlcNAc and tet-induced Mgat6 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions. The Mgat5, EC 2.4.1.155, product is the preferred acceptor for Mgat6, which adds a GlcNAcbeta1,4 branch that is not present in mammals, Mgat6 enhances functionality of N-glycan branching in mammalian cells
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme becomes more stable after elution from a Ni2+-chelating column during purification
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from cDNA library, DNA sequence determination, functional expression in COS-1 cells
gene MGAT6, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat6 HEK293 cells produce increased oligosaccharide levels also observed in HeLa cells, predicted to be the 2,2,4,6 tetra-, 4,2,2,4 tetra- and 4,2,2,4,6 penta-N-glycans, while native 4,2,2,6 tetra are reduced, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schachter, H.; Brockhausen, I.; Hull, E.
High-performance liquid chromatography assays for N-acetylglucosaminyltransferases involved in N- and O-glycan synthesis
Methods Enzymol.
179
351-397
1989
Gallus gallus
brenda
Brockhausen, I.; Hull, E.; Hindsgaul, O.; Schachter, H.; Shah, R.N.; Michnick, S.W.; Carver, J.P.
Control of glycoprotein synthesis. Detection and characterization of a novel branching enzyme from hen oviduct, UDP-N-acetylglucosamine:GlcNAc beta 1-6 (GlcNAc beta 1-2)Man alpha-R (GlcNAc to Man) beta-4-N-acetylglucosaminyltransferase VI
J. Biol. Chem.
264
11211-11221
1989
Anas sp., Gallus gallus, Meleagris gallopavo, no activity in Bos taurus, no activity in Homo sapiens, no activity in Rattus norvegicus, no activity in Sus scrofa
brenda
Taguchi, T.; Kitajima, K.; Inoue, S.; Inoue, Y.; Yang, J.M.; Schachter, H.; Brockhausen, I.
Activity of UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R[GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI (GnT VI) from the ovaries of Oryzias latipes (Medaka fish)
Biochem. Biophys. Res. Commun.
230
533-536
1997
Oryzias latipes
brenda
Sakamoto, Y.; Taguchi, T.; Honke, K.; Korekane, H.; Watanabe, H.; Tano, Y.; Dohmae, N.; Takio, K.; Horii, A.; Taniguchi, N.
Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc):GlcNAcbeta1-6(GlcNAcbeta1-2)-Manalpha1-R[GlcNAc to Man]beta1,4N-acetylglucosaminyltransferase VI
J. Biol. Chem.
275
36029-36034
2000
Gallus gallus (Q9DGD1), Gallus gallus, no activity in african green monkey
brenda
Taguchi, T.; Ogawa, T.; Inoue, S.; Inoue, Y.; Sakamoto, Y.; Korkane, H.; Taniguchi, N.
Purification and characterization of UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]-beta1,4-N-acetylglucosaminyltransferase VI from hen oviduct
J. Biol. Chem.
175
32598-32602
2000
Gallus gallus
-
brenda
Taguchi, T.; Ogawa, T.; Kitajima, K.; Inoue, S.; Inoue, Y.; Ihara, Y.; Sakamoto, Y.; Nagai, K.; Taniguchi, N.
A method for determination of UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI activity using a pyridylaminated tetraantennary oligosaccharide as an acceptor substrate
Anal. Biochem.
255
155-157
1998
Gallus gallus
brenda
Watanabe, T.; Ihara, H.; Miyoshi, E.; Honke, K.; Taniguchi, N.; Taguchi, T.
A specific detection of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins based on the specificity of N-acetylglucosaminyltransferase VI
Glycobiology
16
431-439
2006
Gallus gallus
brenda
Abdel Rahman, A.; Ryczko, M.; Nakano, M.; Pawling, J.; Rodrigues, T.; Johswich, A.; Taniguchi, N.; Dennis, J.
Golgi N-glycan branching N-acetylglucosaminyltransferases I, V and VI promote nutrient uptake and metabolism
Glycobiology
25
225-240
2015
Homo sapiens
brenda
Taguchi, T.; Taniguchi, N.
A method for determination of UDP-GlcNAc: GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man] beta1-4N-acetylglucosaminyltransferase VI activity
Methods Mol. Biol.
1022
299-305
2013
Homo sapiens
brenda
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