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Information on EC 2.4.1.2 - dextrin dextranase
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EC Tree
2.4.1.2 dextrin dextranaseSpecify your search results
Word Map
- 2.4.1.2
- gluconobacter
- oxydans
-
transglucosylation
- capsulatus
- acetobacter
- maltodextrins
- oligosaccharides
- starch
- hydrolysate
-
optimised
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
ddase, dextran dextrinase, dextrin dextranase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DDase
dextran dextrinase
dextrin 6-glucosyltransferase
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glucosyltransferase, dextrin 6-
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dextran dextrinase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
mechanism
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[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
reaction mode and mechanism
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[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-glucan:(1->6)-alpha-D-glucan 6-alpha-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
9032-13-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
1,4-alpha-D-amyloheptaose + (1,6-alpha-D-glucopyranosyl)n
dextran
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?
cellobiose + starch
?
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starch is the donor substrate, cellobiose the acceptor substrate
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?
D-glucosyl-alpha-(1,4)-D-xylose + starch
?
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starch is the donor substrate, D-glucosyl-alpha(1,4)-D-xylose the acceptor substrate
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?
dextran + salicin
?
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dextran is the donor substrate, salicin the acceptor substrate
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?
dextran + salicin
glucosyl-alpha-1,6-salicin + isomaltosyl-alpha-1,6-salicin
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?
gentiobiose + starch
?
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starch is the donor substrate, gentiobiose the acceptor substrate
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?
isomaltooligosaccharide
glucose + isomaltooligosaccharides
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?
isomaltose + salicin
?
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isomaltose is the donor substrate, salicin the acceptor substrate
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?
isomaltose + salicin
glucosyl-alpha-1,6-salicin + isomaltosyl-alpha-1,6-salicin
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?
isomaltose + starch
?
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starch is the donor substrate, isomaltose the acceptor substrate
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?
isoprimeverose + starch
?
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starch is the donor substrate, isoprimeverose the acceptor substrate
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?
kojibiose + starch
?
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starch is the donor substrate, kojibiose the acceptor substrate
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?
laminaribiose + starch
?
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starch is the donor substrate, laminaribiose the acceptor substrate
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?
maltose
dextran
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no activity with kojibiose, sophorose, nigerose, laminaribiose, cellobiose, gentiobiose or trehalose
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?
maltose + salicin
?
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maltose is the donor substrate, salicin the acceptor substrate
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?
maltose + salicin
glucosyl-alpha-1,6-salicin + isomaltosyl-alpha-1,6-salicin
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?
maltose + starch
?
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starch is the donor substrate, maltose the acceptor substrate
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?
maltose-dextran mixture
4-O-alpha-isomaltosyl-D-glucose + 4-O-alpha-isomaltotriosyl-D-glucose + 4-O-alpha-isomaltotetraosyl-D-glucose + 4-O-alpha-isomaltopentaosyl-D-glucose + 4-O-alpha-isomaltohexaosyl-D-glucose
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?
methyl-alpha-D-glucoside + starch
methyl-alpha-isomaltoside + ?
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starch is the donor substrate, methyl-alpha-D-glucoside the acceptor substrate
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?
methyl-beta-D-glucoside + starch
methyl-beta-isomaltoside + ?
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starch is the donor substrate, methyl-beta-D-glucoside the acceptor substrate
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?
nigerose + starch
?
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starch is the donor substrate, nigerose the acceptor substrate
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?
salicin + starch
?
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starch is the donor substrate, salicin, i.e. 2-(hydroxymethyl)phenyl-beta-D-glucopyranoside, the acceptor substrate
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?
sophorose + starch
?
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starch is the donor substrate, sophorose the acceptor substrate
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?
starch + D-glucose
maltose + isomaltose
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no transglucosylation from starch to: D-mannose, D-allose, D-galactose, 3-acetamido-3-deoxy-D-glucose, 6-acetamido-6-deoxy-D-glucose, 1,6-anhydro-D-glucose, D-fructose, D-arabinose or D-ribose
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?
starch + salicin
?
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starch is the donor substrate, salicin the acceptor substrate
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?
sucrose + starch
?
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starch is the donor substrate, sucrose the acceptor substrate
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?
xylosucrose + starch
?
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starch is the donor substrate, xylosucrose the acceptor substrate
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?
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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maltopentaose, maltohexaose, short-chain amylose, soluble starch
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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O-6-deoxy-6-[(2-pyridyl)amino]alpha-D-glucopyranosyl-1,4-maltotriose
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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i.e. dextrin x + dextran y, converts polymerized 1,4-linked glucose units into polymerized 1,6-linked units
successive elongation, i.e. dextrin x-1 + dextran y+1
?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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maltitol
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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substrates are maltooligosaccharides, e.g. maltotriose (G3), maltotetraose (G4)
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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substrates are maltooligosaccharides, e.g. maltotriose (G3), maltotetraose (G4)
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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substrates are maltooligosaccharides, e.g. maltotriose (G3), maltotetraose (G4)
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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poor substrate: maltose (G2)
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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poor substrate: maltose (G2)
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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maltotetraitol
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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maltotetraitol
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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no substrates are unhydrolyzed amylose, amylopectin fractions of corn or potato starch, native oyster or rabbit liver glycogens, Schardinger's beta-dextrin, cycloheptoamylose, maltose, sucrose, glucose 1-phosphate or glucose 6-phosphate, trehalose, alpha-methylglucoside, raffinose, melezitose
successive elongation, i.e. dextrin x-1 + dextran y+1
?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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three transglucosylation action modes: main reaction, disproportional action on maltooligosaccharides and disproportional action on isomaltooligosaccharides
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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enzyme acts on non-reducing terminal glucosyl residues
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?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
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maltose can act as glucosyl group acceptor
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?
4-nitrophenyl-alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
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artificial substrate
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?
4-nitrophenyl-alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
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artificial substrate
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?
4-nitrophenyl-alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
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artificial substrate
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?
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
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dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points
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?
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
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dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points
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?
additional information
?
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substrate specificity, no activity with glucose or maltose, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage, consecutive glucosyl transfer results in formation of high molecular weight dextran, dextran synthesis pathway overview
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?
additional information
?
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donor and acceptor substrate specificity, no activity with trehalose, lactose, or melibiose as acceptor substrates, when starch is the donor substrate, overview, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage or an alpha1,4-linkage
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?
additional information
?
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DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation
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?
additional information
?
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substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose
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?
additional information
?
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substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose
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?
additional information
?
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substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose
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?
additional information
?
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substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose
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?
additional information
?
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DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
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dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points
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?
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
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dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points
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?
additional information
?
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substrate specificity, no activity with glucose or maltose, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage, consecutive glucosyl transfer results in formation of high molecular weight dextran, dextran synthesis pathway overview
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?
additional information
?
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DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation
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?
additional information
?
-
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DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation
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?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
no activation by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Ni2+, Zn2+ and EDTA
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
not inhibited by Ca2+, Co2+, Fe2+, Mg2+, Mn2+, Ni2+, Zn2+, p-chloromercuribenzoate and EDTA
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview
brenda
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optimization of the fermentation medium for DDase production by Gluconaobacter oxydans M5, detailed overview. The optimum medium consisted of glucose 17.670 g/l, maltobiose 30 g/l, tryptone 12.198 g/l, yeast extract 13.528 g/l, ammonium nitrate 15 g/l, copper sulfate 0.01 g/l, zinc sulfate 0.01 g/l, and sodium chloride 0.009 g/l at pH 6.0 and 30°C
brenda
-
optimization of the fermentation medium for DDase production by Gluconaobacter oxydans M5, detailed overview. The optimum medium consisted of glucose 17.670 g/l, maltobiose 30 g/l, tryptone 12.198 g/l, yeast extract 13.528 g/l, ammonium nitrate 15 g/l, copper sulfate 0.01 g/l, zinc sulfate 0.01 g/l, and sodium chloride 0.009 g/l at pH 6.0 and 30°C
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brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE