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UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine = UDP + O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
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-
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-
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UDP-N-acetyl-D-glucosamine + (O-mannopyranosyl-alpha-1,3)-mannopyranosyl-beta-1,4-N-acetyl-glucosamine
UDP + O-mannopyranosyl-alpha-1,3-[N-acetyl-glucosamine-beta-1,4]-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
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i.e. Man2GlcNAc, very low activity
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?
UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside
UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine
UDP + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-(N-acetyl-D-glucosamine-beta-1,4)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
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i.e. Man9GlcNAc oligosaccharide, enzyme catalyzes the transfer of N-acetyl-glucosamine to both alpha- and beta linked mannoses
i.e. Man9GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue attached beta-1,4 to the mannose linked alpha-1,6 to the core mannose in an intersecting position
?
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl
?
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i.e. Man2Me
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-
?
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
UDP + O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
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i.e. Man5GlcNAc oligosaccharide, 20% of activity compared to Man9GlcNAc
i.e. Man5GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue in an intersected and/or bisected position, so that either the core beta-mannose or the branching alpha-mannose residue accepts the transferred N-acetyl-D-glucosamine residue
?
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine
UDP + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6-(N-acetyl-D-glucosamine beta-1,4)]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine
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i.e. Man3GlcNAc
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?
UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside
UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine
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?
UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside
UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine
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trivial name Man3Me
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?
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1.42
methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside
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intersected product
0.65
O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine
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i.e. Man9GlcNAc, intersected product
18.52
O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl
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intersected product
19.48
O-mannopyranosyl-alpha-1,3-mannopyranosyl-beta-1,4-N-acetyl-glucosamine
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bisected product
0.3 - 0.43
O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
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i.e. Man5GlcNAc, depending whether N-acetyl-D-glucosamine residue is intersected or bisected
2.54
O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine
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bisected product
1.2
UDP-N-acetyl-D-glucosamine
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cosubstrate Man9GlcNAc
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2.4.1.197 high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase
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