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Information on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase and Organism(s) Klebsiella oxytoca and UniProt Accession P08704

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.19 cyclomaltodextrin glucanotransferase
IUBMB Comments
Cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed reversibly from starch and similar substrates. Will also disproportionate linear maltodextrins without cyclizing (cf. EC 2.4.1.25, 4-alpha-glucanotransferase).
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This record set is specific for:
Klebsiella oxytoca
UNIPROT: P08704
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The taxonomic range for the selected organisms is: Klebsiella oxytoca
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
cyclodextrin glycosyltransferase, cyclodextrin glucanotransferase, cyclomaltodextrin glucanotransferase, alpha-cgtase, beta-cgtase, toruzyme, cyclodextrin glucosyltransferase, alpha-cyclodextrin glycosyltransferase, cyclodextrin-glycosyltransferase, cyclomaltodextrin glucanyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-1,4-glucan 4-glycosyltransferase, cyclizing
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alpha-cyclodextrin glucanotransferase
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alpha-cyclodextrin glycosyltransferase
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Bacillus macerans amylase
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beta-cyclodextrin glucanotransferase
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beta-cyclodextrin glycosyltransferase
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BMA
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CGTase
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cyclodextrin glucanotransferase
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cyclodextrin glycosyltransferase
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cyclomaltodextrin glucotransferase
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cyclomaltodextrin glycosyltransferase
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gamma-cyclodextrin glycosyltransferase
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konchizaimu
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neutral-cyclodextrin glycosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
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hydrolysis
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transglycosylation
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hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-glucan:(1->4)-alpha-D-glucan 4-alpha-D-[(1->4)-alpha-D-glucano]-transferase (cyclizing)
Cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed reversibly from starch and similar substrates. Will also disproportionate linear maltodextrins without cyclizing (cf. EC 2.4.1.25, 4-alpha-glucanotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9030-09-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
amylopectin + glycosyl acceptor
cycloheptaamylose + cyclohexaamylose + exo-branched cyclohexaamylose
show the reaction diagram
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r
cycloheptaamylose + glycosyl acceptor
?
show the reaction diagram
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r
cyclohexaamylose + glycosyl acceptor
?
show the reaction diagram
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r
soluble starch + glycosyl acceptor
cyclodextrins
show the reaction diagram
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r
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
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strain M5 a1
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDGT_KLEOX
655
1
73025
Swiss-Prot
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 55
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stable up to 45°C, 13.4% activity at 50°C, no activity above 55°C
80
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15 min at 80°C, after incubation for 12 h reactivation to a little restactivity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
M5a1 cloned and expressed
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
the enzyme can be applied in biotechnology for the production of cyclodextrins and oligosaccharides with novel properties
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bender, H.
Cyclodextrin glucanotransferase from Klebsiella pneumoniae. 1. Formation, purification and properties of the enzyme from Klebsiella pneumoniae M 5 al
Arch. Microbiol.
111
271-282
1977
Priestia megaterium, Paenibacillus macerans, Klebsiella oxytoca, Klebsiella pneumoniae, Priestia megaterium No5, Klebsiella pneumoniae M5
Manually annotated by BRENDA team
Aoki, H.; Yao, D.; Misawa, M.
Production and characterization of a thermostable bacterial cyclodextrin glycosyltransferase
Prog. Biotechnol.
3
81-93
1987
Geobacillus stearothermophilus, Paenibacillus macerans, Bacillus sp. (in: Bacteria), Klebsiella oxytoca, Bacillus sp. (in: Bacteria) IT25
-
Manually annotated by BRENDA team
Akimaru, K.; Yagi, T.; Yamamoto, S.
Purification and properties of Bacillus coagulans cyclomaltodextrin glucanotransferase
J. Ferment. Bioeng.
71
322-328
1991
Geobacillus stearothermophilus, Niallia circulans, Weizmannia coagulans, Priestia megaterium, Bacillus subtilis, Paenibacillus macerans, Bacillus ohbensis, Bacillus sp. (in: Bacteria), Klebsiella oxytoca, Klebsiella pneumoniae, Geobacillus stearothermophilus TC-60, Bacillus subtilis 313
-
Manually annotated by BRENDA team
Fujiwara, S.; Kakihara, H.; Sakaguchi, K.; Imanaka, T.
Analysis of mutations in cyclodextrin glucanotransferase from Bacillus stearothermophilus which affect cyclization characteristics and thermostability
J. Bacteriol.
174
7478-7481
1992
Geobacillus stearothermophilus, Niallia circulans, Paenibacillus macerans, Klebsiella oxytoca, Geobacillus stearothermophilus NO2, Geobacillus stearothermophilus TC-91, Niallia circulans 8
Manually annotated by BRENDA team
Fujiwara, S.; Kakihara, H.; Woo, K.B.; Lejeune, A.; Kanemoto, M.; Sakaguchi, K.; Imanaka, T.
Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme
Appl. Environ. Microbiol.
58
4016-4025
1992
Bacillus licheniformis, Bacillus sp. (in: Bacteria), Bacillus subtilis, Bacillus subtilis NA-1, Geobacillus stearothermophilus, Geobacillus stearothermophilus (P31797), Geobacillus stearothermophilus NO2, Klebsiella oxytoca, Klebsiella pneumoniae, Niallia circulans, Niallia circulans 8, Paenibacillus macerans, Paenibacillus macerans (P04830), Paenibacillus macerans IAM1243, Priestia megaterium
Manually annotated by BRENDA team
Tomita, K.; Kaneda, M.; Kawamura, K.; Nakanishi, K.
Purification and properties of a cyclodextrin glucanotransferase from Bacillus autolyticus 11149 and selective formation of beta-cyclodextrin
J. Ferment. Bioeng.
75
89-92
1993
Geobacillus stearothermophilus, Bacillus autolyticus, Niallia circulans, Paenibacillus macerans, Bacillus sp. (in: Bacteria), Klebsiella oxytoca, Bacillus sp. (in: Bacteria) AL-6, Bacillus autolyticus 11149
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Manually annotated by BRENDA team
Ferrarotti, S.A.; Rosso, A.M.; Marechal, M.A.; Krymkiewicz, N.; Marechal, L.R.
Isolation of two strains (S-R type) of Bacillus circulans and purification of a cyclomaltodextrin-glucanotransferase
Cell. Mol. Biol.
42
653-657
1996
Geobacillus stearothermophilus, Alkalihalobacillus alcalophilus, Niallia circulans, Weizmannia coagulans, Priestia megaterium, Bacillus subtilis, Lederbergia lentus, Paenibacillus macerans, Bacillus sp. (in: Bacteria), Klebsiella oxytoca, Niallia circulans DF9
Manually annotated by BRENDA team
Tonkova, A.
Bacterial cyclodextrin glucanotransferase
Enzyme Microb. Technol.
22
678-686
1998
Bacillus cereus, Bacillus cereus NCIMB 13123, Bacillus licheniformis, Bacillus ohbensis, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 1011, Bacillus sp. (in: Bacteria) AL-6, Bacillus sp. (in: Bacteria) INMIA 1919, Bacillus sp. (in: Bacteria) INMIA A7/1, Bacillus sp. (in: Bacteria) INMIA T4, Bacillus sp. (in: Bacteria) INMIA t6, Geobacillus stearothermophilus, Geobacillus stearothermophilus N2, Klebsiella oxytoca, Klebsiella pneumoniae, Lederbergia lentus, Lysinibacillus sphaericus, Lysinibacillus sphaericus ATCC 7055, Micrococcus luteus, Niallia circulans, Niallia circulans 8, Paenibacillus macerans, Paenibacillus macerans IAM1243, Priestia megaterium, Priestia megaterium No5, Salimicrobium halophilum, Salimicrobium halophilum INMIA-3849, Thermoanaerobacterium thermosulfurigenes, Thermoanaerobacterium thermosulfurigenes EM1, Weizmannia coagulans
-
Manually annotated by BRENDA team
Tachibana, Y.; Kuramura, A.; Shirasaka, N.; Suzuki, Y.; Yamamoto, T.; Fujiwara, S.; Takagi, M.; Imanaka, T.
Purification and characterization of an extremely thermostable cyclomaltodextrin glucanotransferase from a newly isolated hyperthermophilic archaeon, a Thermococcus sp
Appl. Environ. Microbiol.
65
1991-1997
1999
Bacillus licheniformis (P14014), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) (P31746), Bacillus sp. (in: Bacteria) 1011, Brevibacterium sp., Brevibacterium sp. 9605, Geobacillus stearothermophilus, Geobacillus stearothermophilus (P31797), Klebsiella oxytoca, Klebsiella pneumoniae, Paenibacillus macerans, Paenibacillus macerans (P04830), Thermoanaerobacter sp., Thermoanaerobacterium thermosulfurigenes, Thermoanaerobacterium thermosulfurigenes (P26827), Thermococcus sp., Thermococcus sp. B1001
Manually annotated by BRENDA team
Rashid, N.; Cornista, J.; Ezaki, S.; Fukui, T.; Atomi, H.; Imanaka, T.
Characterization of an archaeal cyclodextrin glucanotransferase with a novel C-terminal domain
J. Bacteriol.
184
777-784
2002
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 1011, Geobacillus stearothermophilus, Geobacillus stearothermophilus (P31797), Klebsiella oxytoca (P08704), Niallia circulans, Niallia circulans (P43379), Niallia circulans 251, Niallia circulans 251 (P43379), Paenibacillus macerans (P31835), Thermoanaerobacterium thermosulfurigenes, Thermoanaerobacterium thermosulfurigenes EM1, Thermococcus kodakarensis (Q8X268), Thermococcus sp., Thermococcus sp. (Q9UWN2), Thermococcus sp. B1001, Thermococcus sp. B1001 (Q9UWN2)
Manually annotated by BRENDA team
Qi, Q.; Zimmermann, W.
Cyclodextrin glucanotransferase: from gene to applications
Appl. Microbiol. Biotechnol.
66
475-485
2005
Bacillus licheniformis (P14014), Bacillus ohbensis (P27036), Bacillus sp. (in: Bacteria) (O82984), Bacillus sp. (in: Bacteria) (P05618), Bacillus sp. (in: Bacteria) (P17692), Bacillus sp. (in: Bacteria) (P30921), Bacillus sp. (in: Bacteria) (P31747), Bacillus sp. (in: Bacteria) (Q59239), Bacillus sp. (in: Bacteria) 1011 (P05618), Bacillus sp. (in: Bacteria) 1018 (P17692), Bacillus sp. (in: Bacteria) 17.1 (P30921), Bacillus sp. (in: Bacteria) 38-2 (P30921), Bacillus sp. (in: Bacteria) 6.6.3 (P31747), Bacillus sp. (in: Bacteria) A2-5a (O82984), Bacillus sp. (in: Bacteria) KC201 (Q59239), Brevibacillus brevis (O30565), Brevibacillus brevis CD162 (O30565), Cytobacillus firmus, Cytobacillus firmus 290-3, Evansella clarkii (Q8L3E0), Evansella clarkii 7384 (Q8L3E0), Geobacillus stearothermophilus (P31797), Klebsiella oxytoca (P08704), Klebsiella oxytoca M5a1 (P08704), Niallia circulans (P30920), Niallia circulans (P43379), Niallia circulans (Q9F5W3), Niallia circulans 251 (P43379), Niallia circulans 8 (P30920), Niallia circulans A11 (Q9F5W3), Nostoc sp. (Q8RMG0), Nostoc sp. 9229 (Q8RMG0), Paenibacillus macerans (P31835), Salipaludibacillus agaradhaerens, Salipaludibacillus agaradhaerens (Q7X3T0), Salipaludibacillus agaradhaerens DSM 8721 (Q7X3T0), Salipaludibacillus agaradhaerens DSM 9948, Salipaludibacillus agaradhaerens LS-3C, Streptococcus pyogenes, Thermoanaerobacter sp., Thermoanaerobacterium thermosulfurigenes (P26827), Thermococcus kodakarensis (Q8X268), Thermococcus sp. (Q9UWN2), Thermococcus sp. B1001 (Q9UWN2), Xanthomonas axonopodis, Xanthomonas campestris
Manually annotated by BRENDA team