in vitro, enzyme CsUGT76F1 can function as flavonoid 3-O-, 7-O-, and 3,7-O-glucosyltransferase, and as 7-O-rhamnosyltransferase with a broad range of flavonoid substrates that can be glucosylated at their 3- and/or 7-hydrogen sites by the recombinant enzyme, including flavanones, flavonols, and flavons, e.g. hesperetin, naringenin, diosmetin, quercetin, and kaempferol as acceptor substrates, and UDP-glucose and UDP-rhamnose as the sugar donors. Mass spectrometric product analysis (UPLC-Q-TOF-MS). The recombinant UGT can only accept UDP-rhamnose to rhamnosylate the 7-hydrogen position of quercetin and kaempferol, but not to catalyze the rhamnosylation of hesperetin, naringenin, and diosmetin. No flavonoid O-rutinoside (rhamnosyl-alpha-1,6-glucose) or O-neohesperidose (rhamnosyl-alpha-1,2-glucose) products are detected
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DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression in Nicotiana tabacum using the Agrobacterium tumefaciens strain EHA105 transfection method