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Information on EC 2.4.1.175 - glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
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EC Tree
2.4.1.175 glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferaseIUBMB Comments
Involved in the biosynthesis of chondroitin sulfate. The human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase (EC 2.4.1.226, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase) activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli.
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Word Map
- 2.4.1.175
- glycosaminoglycans
- n-acetylgalactosamine
- udp-galnac
-
glcua
-
sulfotransferases
- medicine
-
glucuronyltransferase
- hexasaccharide
- udp-glca
- udp-sugars
-
d-glucuronic
-
chemo-enzymatically
- diagnostics
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
=
+
+
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
=
+
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
+
=
+
+
[protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
=
+
[protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n+1-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Synonyms
chondroitin polymerase, chsy-2, chondroitin synthase-1, csgalnact-2, n-acetylgalactosaminyltransferase ii, chondroitin synthase 1, cs synthase-1, cs glycosyltransferase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-chondroitin, II
-
-
-
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ChGn-2
chondroitin galactosaminyltransferase
chondroitin polymerase
chondroitin sulfate N-acetylgalactosaminyltransferase-2
chondroitin sulfate synthase 1
chondroitin sulfate synthase-1
chondroitin sulfate synthase-2/chondroitin polymerizing factor
chondroitin sulfate synthase-3
chondroitin synthase
chondroitin synthase-2
based on the experimental results chondroitin sulfate synthase-3 is renamed into chondroitin synthase-2
chondroitin synthase-3
based on the experimental results CSGlcA-T renamed into chondroitin synthase-3
CHSY1
CpCS
Cpha266_2341
CSS2
CSS3
glucuronyl-N-acetylgalactosaminylproteoglycan beta1,4-N-acetylgalactosaminyltransferase
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K4CP
N-acetylgalactosaminyltransferase II
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UDP-N-acetyl-D-galactosamine:D-glucuronyl-N-acetyl-1,3-beta-D-galactosaminylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase
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uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferasec II
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additional information
chondroitin synthase
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-
-
-
additional information
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the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity
additional information
-
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity
-
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
-
additional information
the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity
additional information
the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174
additional information
the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174
additional information
the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174
additional information
the enzyme shows no 3-beta-glucuronosyltransferase activity, EC 2.4.1.226
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity
additional information
the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
additional information
the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli.
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n+1-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
(2)
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-
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UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
enzyme contains 2 distinct catalytic site for GalNAc and GlcNAc transferase activity
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
(1)
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
Involved in the biosynthesis of chondroitin sulfate. The human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase (EC 2.4.1.226, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase) activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli.
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CAS REGISTRY NUMBER
COMMENTARY
96189-40-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-thrombomodulin + UDP-N-acetyl-D-galactosamine + UDP-glucuronic acid
?
-
-
-
?
chondroitin + UDP-glucuronic acid
?
chondroitin is the best substrate
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-
?
chondroitin sulfate A10 + UDP-GalNAc
?
no activity with UDP-glucuronic acid
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-
?
chondroitin sulfate A11 + UDP-glucuronic acid
?
no activity with UDP-GalNAc
-
-
?
chondroitin sulfate C10 + UDP-GalNAc
?
no activity with UDP-glucuronic acid
-
-
?
chondroitin sulfate C11 + UDP-glucuronic acid
?
no activity with UDP-GalNAc
-
-
?
GlcUAbeta1-3Galbeta1-O-C2H4NH-benzyloxycarbonyl + UDP-glucuronic acid
?
-
-
-
?
GlcUAbeta1-3Galbeta1-O-C2H4NH-benzyloxycarbonyl + UDP-N-acetyl-D-galactosamine
?
-
-
-
?
GlcUAbeta1-3Galbeta1-O-naphthalenemethanol + UDP-glucuronic acid
?
-
-
-
?
GlcUAbeta1-3Galbeta1-O-naphthalenemethanol + UDP-N-acetyl-D-galactosamine
?
-
-
-
?
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
UDP + [GlcA-GalNAc]n+1-pyridylamine
-
-
-
-
?
UDP-GalNAc + 4-nitrophenyl D-glucuronide
UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine 4-sulfate)4
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
i.e. chondroitin sulfate octasaccharide, glycosylated at 32% the rate of chondroitin octasaccharide
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-glucuronyl-N-acetyl-1,3-beta-D-galactosamine
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)8
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7
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i.e. chondroitin hexadecasaccharide, glycosylated at 15% the rate of decamer glycosylation
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-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-glucosamine)4
UDP + ?
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i.e. hyaluronate octasaccharide, glycosylated at 25% the rate of chondroitin octasaccharide
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
UDP-N-acetyl-alpha-D-galactosamine + chondroitin hexasaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin hexasaccharide
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
UDP-N-acetyl-alpha-D-galactosamine + chondroitin pentasaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin pentasaccharide
UDP-N-acetyl-alpha-D-galactosamine + chondroitin polysaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin polysaccharide
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
UDP-N-acetyl-alpha-D-galactosamine + [GlcA-GalNAc]n-pyridylamine
UDP + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
UDP-N-acetyl-beta-D-galactosamine + chondroitin sulfate hexasaccharide
?
-
-
-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
UDP-N-acetyl-D-galactosamine + chondroitin polysaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin polysaccharide
best acceptor substrate
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate C
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide
UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide
UDP-GalNAc + 4-nitrophenyl D-glucuronide
UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
-
-
?
UDP-GalNAc + 4-nitrophenyl D-glucuronide
UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
-
-
?
UDP-GalNAc + 4-nitrophenyl D-glucuronide
UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-glucuronyl-N-acetyl-1,3-beta-D-galactosamine
-
i.e. chondroitin tetrasaccharide, glycosylated at 40% the rate of decamer glycosylation
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-glucuronyl-N-acetyl-1,3-beta-D-galactosamine
minimal required acceptor substrate chain length
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
minimal chain length required for activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
i.e. chondroitin hexasaccharide, glycosylated at 60% the rate of decamer glycosylation
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
low activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
i.e. chondroitin octasaccharide, glycosylated at 75% the rate of decamer glycosylation
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
low activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
-
i.e. chondroitin decasaccharide, best substrate
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
-
i.e. chondroitin dodecasaccharide, glycosylated at about 60% the rate of decamer glycosylation
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
-
i.e. chondroitin tetradecasaccharide, glycosylated at about 45% the rate of decamer glycosylation
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7
UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin hexasaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin hexasaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin hexasaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin hexasaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
involved in chondroitin biosynthesis
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
involved in chondroitin biosynthesis
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
involved in chondroitin biosynthesis
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin pentasaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin pentasaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin pentasaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin pentasaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin polysaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin polysaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin polysaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin polysaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + [GlcA-GalNAc]n-pyridylamine
UDP + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + [GlcA-GalNAc]n-pyridylamine
UDP + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
-
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
-
weak activity
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
-
weak activity
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
best acceptor substrate
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
no substrates are glucuronyl-N-acetyl-1,3-beta-galactosamine or glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-N-acetyl-1,3-beta-D-galactosaminyl-N-acetyl-galactosaminol
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
long polymers are not formed
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
acceptor substrates longer than a tetrasaccharide are absolutely required
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
long polymers are not formed
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
acceptor substrates longer than a tetrasaccharide are absolutely required
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
low activity
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
best acceptor substrate
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
best acceptor substrate
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage
-
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
high specificity for sugar donor substrates
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
high specificity for sugar donor substrates
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor
transfer in beta-1,4-linkage
?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate C
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C
-
no activity
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate C
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C
-
no activity
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate C
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C
11% activity compared to chondroitin
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate C
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C
no activity
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
-
no activity
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
acceptor substrates longer than a tetrasaccharide are absolutely required
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
-
no activity
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
acceptor substrates longer than a tetrasaccharide are absolutely required
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
-
-
?
UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
no activity
-
-
?
UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide
UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide
-
the native K4 oligosaccharide, containing a fructose residue branch at C-3 of each GlcNAc residue of the chondroitin backbone, is no substrate
-
?
UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide
UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide
-
in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor, but long polymers are not formed
-
?
UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide
UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide
-
the native K4 oligosaccharide, containing a fructose residue branch at C-3 of each GlcNAc residue of the chondroitin backbone, is no substrate
-
?
additional information
?
-
GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview. GlcA-pNP can serve as the acceptor for ApCS
-
-
-
additional information
?
-
GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview. GlcA-pNP can serve as the acceptor for ApCS
-
-
-