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Synonyms
sterol glucosyltransferase, atg26, ugt80b1, ugt80a2, sgtl1, ugt51, udpg-sgtase, sterol glycosyltransferase, sgtl2, udp-glucose:sterol glucosyltransferase,
more
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UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
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-
-
?
3beta-hydroxy-16,17alpha-epoxypregnenolone + UDP-alpha-D-glucose
3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside + UDP
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-
-
-
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
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-
-
-
?
5alpha-cholestan-3beta-ol + UDP-glucose
UDP + 3beta,5alpha-cholestan 3-beta-D-glucoside
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-
-
-
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
-
-
-
-
?
brassicasterol + UDP-glucose
UDP + brassicasterol 3-beta-D-glucoside
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-
-
-
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
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-
-
-
?
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
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-
-
-
?
ergosterol + UDP-glucose
UDP + ergosterol 3-beta-D-glucoside
-
-
-
-
?
pregnenolene + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
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-
-
-
?
solasodine + UDP-glucose
UDP + solasodine 3-beta-D-glucoside
-
-
-
-
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
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-
-
-
?
UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
UDP + 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside
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-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
-
-
-
-
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
UDP-glucose + transandrosterone
UDP + transandrosterone 3beta-D-glucoside
-
-
-
-
?
additional information
?
-
-
the enzyme only shows activity with sterols having hydroxyl group at C-3 position. Utilises only UDP-glucose, UDP-galactose cannot serve as the sugar donor.
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme exhibits equal activity to the purified protein
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
-
-
?
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evolution
UGT80 proteins belong to a third family that is hypothesized to be involved in sterol glucoside synthesis, phylogenetic tree of UGT80- and UGT713-related proteins, overview
malfunction
inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis thaliana resistance to Botrytis cinerea infection, which correlates with increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Analysis of the response to necrotrophic fungus Botrytis cinerea in an Arabidopsis thaliana mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases, UGT80A2 and UGT80B1. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also upregulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants
metabolism
crosstalk between hormone signaling pathways, particularly those mediated by salicylate and jasmonate, has been found to contribute to plant resistance to different types of pathogens. The results suggest that the salicylate-mediated defense pathway is not involved in the response of the ugt80A2;B1 mutant to Bortrytis cinerea infection. But camalexin and, probably, also indole glucosinolates are actively involved in the enhanced resistance of the ugt80A2;B1 mutant to Bortrytis cinerea infection. The synthesis of alkylglucosinolates in the ugt80A2;B1 mutant is not affected by Bortrytis cinerea infection
physiological function
UGT80A2 accounts for most of the sitosteryl and stigmasteryl glucoside production in seeds
metabolism
crosstalk between hormone signaling pathways, particularly those mediated by salicylate and jasmonate, has been found to contribute to plant resistance to different types of pathogens. The results suggest that the salicylate-mediated defense pathway is not involved in the response of the ugt80A2;B1 mutant to Bortrytis cinerea infection. But camalexin and, probably, also indole glucosinolates are actively involved in the enhanced resistance of the ugt80A2;B1 mutant to Bortrytis cinerea infection. The synthesis of alkylglucosinolates in the ugt80A2;B1 mutant is not affected by Bortrytis cinerea infection
additional information
sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only
evolution
phylogenetic tree of UGT80- and UGT713-related proteins, overview
evolution
UGT80 proteins belong to a third family that is hypothesized to be involved in sterol glucoside synthesis, phylogenetic tree of UGT80- and UGT713-related proteins, overview
evolution
the genome of Arabidopsis thaliana contains two genes coding for UDP-Glc:sterol-glucosyltransferases, UGT80A2 and UGT80B1, and studies of mutant lines indicate that they are only partially redundant
malfunction
ugt80B1 mutants displaya a significant reduction only in campesteryl, brassicasteryl, and cholesteryl glucosides, but not sitosteryl or stigmasteryl glucosides
malfunction
inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis thaliana resistance to Botrytis cinerea infection, which correlates with increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Analysis of the response to necrotrophic fungus Botrytis cinerea in an Arabidopsis thaliana mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases, UGT80A2 and UGT80B1. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also upregulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants
malfunction
the plasma membrane cell fate regulator, SCRAMBLED (SCM), is mislocalized in ugt80B1 mutants, underscoring the aberrant root epidermal cell patterning. GFP-tagged SCM is localized to the cytoplasm in a non cell type dependent manner instead of the hair (H) cell plasma membrane in these mutants. Abnormal root hair cell patterning in ugt80B1 mutants is likely the direct result of expression of GL2 in H cell files in this mutant. The aberrant expression of GL2 is caused by the mislocalization of SCM away from the cell periphery, reducing the capacity of the receptor to mediate positional information to the cell. Reductions in specific sterol glucosides might be responsible for the disruption of cell fate regulators in these ugt80B1 mutants. The mislocalization of SCM to the cytoplasm can point to a role for sterol glucosides in vesicular trafficking or plasma membrane protein targeting. Deficiencies in specific sterol glucosides are sufficient to disrupt normal cell function and point to a possible role for sterol glucosides in cargo transport and/or protein targeting to the plasma membrane. Aberrant subcellular localization of SCM:GFP in ugt80B1 epidermal cells from the elongation zone of the root
physiological function
no role in steryl glucoside synthesis for UGT713B1/At5g24750
physiological function
UGT80B1 plays a specialized role in steryl glucoside synthesis
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additional information
mutation of isoform UGT80B1 principally alters embryonic development and seed suberin accumulation and cutin formation in the seed coat, leading to abnormal permeability and tetrazolium salt uptake, mutations in UGT80A2 and USG80B1 genes result in reduced seed size, transparent testa, and salt uptake phenotypes
additional information
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
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construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
generation of a ugt80A2;B1 double mutant that is more resistant to infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. Mutant phenotype, overview
additional information
generation of a ugt80A2;B1 double mutant that is more resistant to infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. Mutant phenotype, overview
additional information
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
-
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
-
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
generation of a ugt80A2;B1 double mutant that is more resistant ot infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level, Mutant phenotype, overview
additional information
generation of a ugt80A2;B1 double mutant that is more resistant ot infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level, Mutant phenotype, overview
additional information
generation of ugt80B1 mutants. Sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only. Root hair patterning and GL2 expression are aberrant in ugt80B1 mutants. Expression of upstream cell fate regulators, SCM and WER, is altered in ugt80B1 mutants. Phenotype, overview. The ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype
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expression in Escherichia coli
gene At3g07020, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern. UGT80A2 expressing cells displays a greater than 100fold increase in cholesteryl glucoside production compared with the negative control
gene UGT80A2, quantitative reverse-transcription PCR expression analysis
expressed in Escherichia coli strain DH5alpha
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gene At1g43620, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern
gene At5g24750, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression patterns. UGT713B1 displays little or no activity
gene UGT80B1, quantitative reverse-transcription PCR expression analysis
gene UGT80B1, recombinant expression of native promoter and 35 S driven GFP-tagged wild-type and mutant enzymes, the ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype
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Warnecke, D.C.; Baltrusch, M.; Buck, F.; Wolter, F.P.; Heinz, E.
UDP-glucose:sterol glucosyltransferase: cloning and functional expression in Escherichia coli
Plant Mol. Biol.
35
597-603
1997
Arabidopsis thaliana (Q9M8Z7), Avena sativa (O22678), Avena sativa ugt80A1 (O22678)
brenda
Sharma, L.K.; Madina, B.R.; Chaturvedi, P.; Sangwan, R.S.; Tuli, R.
Molecular cloning and characterization of one member of 3beta-hydroxy sterol glucosyltransferase gene family in Withania somnifera
Arch. Biochem. Biophys.
460
48-55
2007
Arabidopsis thaliana, Withania somnifera (Q2I015), Withania somnifera
brenda
DeBolt, S.; Scheible, W.R.; Schrick, K.; Auer, M.; Beisson, F.; Bischoff, V.; Bouvier-Nave, P.; Carroll, A.; Hematy, K.; Li, Y.; Milne, J.; Nair, M.; Schaller, H.; Zemla, M.; Somerville, C.
Mutations in UDP-glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds
Plant Physiol.
151
78-87
2009
Arabidopsis thaliana (Q9M8Z7)
brenda
Stucky, D.F.; Arpin, J.C.; Schrick, K.
Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
J. Exp. Bot.
66
189-201
2015
Arabidopsis thaliana (F4KII1), Arabidopsis thaliana (Q9M8Z7), Arabidopsis thaliana (Q9XIG1), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (F4KII1), Arabidopsis thaliana Col-0 (Q9M8Z7), Arabidopsis thaliana Col-0 (Q9XIG1)
brenda
Castillo, N.; Pastor, V.; Chavez, A.; Arro, M.; Boronat, A.; Flors, V.; Ferrer, A.; Altabella, T.
Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
Front. Plant Sci.
10
1162
2019
Arabidopsis thaliana (Q9M8Z7), Arabidopsis thaliana (Q9XIG1), Arabidopsis thaliana Ws-0 (Q9M8Z7), Arabidopsis thaliana Ws-0 (Q9XIG1)
brenda
Pook, V.G.; Nair, M.; Ryu, K.; Arpin, J.C.; Schiefelbein, J.; Schrick, K.; DeBolt, S.
Positioning of the SCRAMBLED receptor requires UDP-Glc sterol glucosyltransferase 80B1 in Arabidopsis roots
Sci. Rep.
7
5714
2017
Arabidopsis thaliana (Q9XIG1), Arabidopsis thaliana Col-0 (Q9XIG1)
brenda