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Information on EC 2.4.1.150 - N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase and Organism(s) Rattus norvegicus and UniProt Accession Q8CH87

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IUBMB Comments
The enzyme acts on poly-N-acetyllactosamine [glycan chains of beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine units connected by beta(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by beta(1,6)-linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q8CH87
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
gcnt2, i-branching enzyme, glucosaminyl (n-acetyl) transferase 2, i-branching beta-1,6-n-acetylglucosaminyltransferase, i-branching n-acetylglucosaminyltransferase, i n-acetylglucosaminyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
distally acting I-branching beta1,6-N-acetylglucosaminyltransferase
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acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->6-
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beta1-6GnT
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Galbeta1-->4GlcNAc-R beta1-->6 N-acetylglucosaminyltransferase
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Gcnt2 gene product
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I N-acetylglucosaminyltransferase
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I-branching beta-1,6-N-acetylglucosaminyltransferase
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N-acetylglucosaminyltransferase
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N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
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UDP-GlcNAc:Gal-R, beta-D-6-N-acetylglucosaminyltransferase
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UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminide 6-beta-N-acetyl-D-glucosaminyltransferase
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uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->6-acetylglucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminide 6-beta-N-acetylglucosaminyltransferase (configuration-inverting)
The enzyme acts on poly-N-acetyllactosamine [glycan chains of beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine units connected by beta(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by beta(1,6)-linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.
CAS REGISTRY NUMBER
COMMENTARY hide
85638-40-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-3Galbeta1-4Glc-2-aminopyridine
UDP + GlcNAcbeta1-3(GlcNAcbeta1-6)Galbeta1-4Glc-2-aminopyridine
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan
?
show the reaction diagram
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might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans
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?
UDP-N-acetyl-D-glucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
show the reaction diagram
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poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan
?
show the reaction diagram
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might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans
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?
additional information
?
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the enzyme should mostly be responsible for making distal I-branch structures on poly-N-acetyllactosamine sequences in small intestine, as well as making mucin core 2 and core 4 structures, it alos has high C2/C4GnT activities
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
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CdCl2, slightly stimulates
Mg2+
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MgCl2, slightly stimulates
Mn2+
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MnCl2, 10-25 mM for optimal activity required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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cIGnT
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GCNT3_RAT
437
1
50642
Swiss-Prot
Secretory Pathway (Reliability: 1)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA inserted into a mammalian expression vector, pSVK3 and overexpressed in COS-1 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Van den Eijnden, D.H.; Winterwerp, H.; Smeeman, P.; Schiphorst, W.E.C.M.
Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1-3 and beta 1-6 N-acetylglucosaminyltransferase activity
J. Biol. Chem.
258
3435-3437
1983
Rattus norvegicus
Manually annotated by BRENDA team
Fukuda, M.
beta6-N-acetylglucosaminyltransferase (IGnT)
Handbook of Glycosyltransferases and Related Genes
2002
125-132
2002
Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
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Manually annotated by BRENDA team
Korekane, H.; Taguchi, T.; Sakamoto, Y.; Honke, K.; Dohmae, N.; Salminen, H.; Toivonen, S.; Helin, J.; Takio, K.; Renkonen, O.; Taniguchi, N.
Purification and cDNA cloning of UDP-GlcNAc:GlcNAcbeta1-3Galbeta1-4Glc(NAc)-R [GlcNAc to Gal] beta1,6N-acetylglucosaminyltransferase from rat small intestine: A major carrier of dIGnT activity in rat small intestine
Glycobiology
13
387-400
2003
Rattus norvegicus (Q8CH87)
Manually annotated by BRENDA team
Li, C.; Wu, Q.
Adaptive evolution of multiple-variable exons and structural diversity of drug-metabolizing enzymes
BMC Evol. Biol.
7
69
2007
Canis lupus familiaris, Danio rerio, Gallus gallus, Homo sapiens, Macaca mulatta, Monodelphis domestica, Mus musculus, Pan troglodytes, Rattus norvegicus, Xenopus tropicalis
Manually annotated by BRENDA team