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Information on EC 2.4.1.15 - alpha,alpha-trehalose-phosphate synthase (UDP-forming) and Organism(s) Candida albicans and UniProt Accession Q92410

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EC Tree
IUBMB Comments
See also EC 2.4.1.36 [alpha,alpha-trehalose-phosphate synthase (GDP-forming)].
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This record set is specific for:
Candida albicans
UNIPROT: Q92410
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The taxonomic range for the selected organisms is: Candida albicans
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
trehalose-6-phosphate synthase, transglucosylase, trehalose 6-phosphate synthase, trehalose phosphate synthase, tps-2, trehalose-6-phosphate synthase 1, t6p synthase, trehalose-6-p synthase, trehalose-6p synthase, t-6-p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trehalose-6-phosphate synthase
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alpha,alpha-trehalose phosphate synthase (UDP-forming)
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glucosyltransferase, uridine diphosphoglucose phosphate
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phosphotrehalose-uridine diphosphate transglucosylase
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TPS
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transglucosylase
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trehalose 6-phosphate synthase
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trehalose 6-phosphate synthetase
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trehalose phosphate synthase
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trehalose phosphate synthetase
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trehalose phosphate-uridine diphosphate glucosyltransferase
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trehalose-6-phosphate synthase
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trehalose-P synthetase
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trehalosephosphate-UDP glucosyl transferase
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UDPglucose-glucose-phosphate glucosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-glucose-6-phosphate 1-alpha-D-glucosyltransferase
See also EC 2.4.1.36 [alpha,alpha-trehalose-phosphate synthase (GDP-forming)].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-07-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
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?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
additional information
?
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trehalose is able to protect the integrity of the cells against a variety of environmental stresses such as desiccation, dehydration, heat, cold, and oxidation, probably via reduction of protein denaturation through protein-trehalose interactions, the enzyme might be a radical scavenger
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
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-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
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step in trehalose biosynthesis
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?
additional information
?
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trehalose is able to protect the integrity of the cells against a variety of environmental stresses such as desiccation, dehydration, heat, cold, and oxidation, probably via reduction of protein denaturation through protein-trehalose interactions, the enzyme might be a radical scavenger
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
5 mM used in assay conditions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
validoxylamine A
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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disruption of the TPS2 gene encoding the only trehalose 6-phosphate phosphatase activity in Candida albicans causes a pleiotropic defective phenotype, maintaining the cell wall integrity and the ability to form chlamydospores. A homozygous tps2Delta/tps2Delta shows reduced growth at high temperatures and a marked sensitivity to heatshock and severe oxidative exposure. Exponential tps2Delta null cells display an adaptive response to oxidative stress as well as cross-tolerance between temperature and oxidative stress. Differential measurement of trehalose and trehalose 6-phosphate, revealed a significant accumulation of trehalose 6-phosphate in tps2Delta cells, which is enhanced after oxidative exposure. The level of trehalose 6-phosphate in parental cells is virtually undetectable, and oxidative treatment only induces the synthesis of free trehalose
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, and 40% (w/v) polyethylene glycol 400
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D379A
inactive
E387A
inactive
K285A
inactive
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex S200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
a transitory increase in the expression of TPS2 and TPS1 genes is promoted in wild-type cells inresponse to acute, but not to gentleoxidative exposure
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chen, Q.; Haddad, G.G.
Role of trehalose phosphate synthase and trehalose during hypoxia: from flies to mammals
J. Exp. Biol.
207
3125-3129
2004
Candida albicans, Drosophila melanogaster, Escherichia coli, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Martinez-Esparza, M.; Martinez-Vicente, E.; Gonzalez-Parraga, P.; Ros, J.M.; Garcia-Penarrubia, P.; Argueelles, J.C.
Role of trehalose-6P phosphatase (TPS2) in stress tolerance and resistance to macrophage killing in Candida albicans
Int. J. Med. Microbiol.
299
453-464
2009
Candida albicans
Manually annotated by BRENDA team
Miao, Y.; Tenor, J.L.; Toffaletti, D.L.; Maskarinec, S.A.; Liu, J.; Lee, R.E.; Perfect, J.R.; Brennan, R.G.
Structural and in vivo studies on trehalose-6-phosphate synthase from pathogenic fungi provide insights into its catalytic mechanism, biological necessity, and potential for novel antifungal drug design
mBio
8
e00643-17
2017
Aspergillus fumigatus (Q4WLM9), Aspergillus fumigatus, Candida albicans (Q92410), Candida albicans, Aspergillus fumigatus Af293 (Q4WLM9)
Manually annotated by BRENDA team