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Information on EC 2.4.1.149 - N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
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EC Tree
2.4.1.149 N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferaseIUBMB Comments
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
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Word Map
- 2.4.1.149
- glycans
-
muscular
- dystrophy
- glycosyltransferases
- dystroglycanopathies
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walker-warburg
-
bulb
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olfactory
- alpha-dystroglycan
- gmppb
- keratan
-
laminin-binding
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pathfinding
-
glomeruli
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pomgnt1
- medicine
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
b3gnt3, b3gnt1, beta3gnt7, beta3gnt1, beta-1,3-n-acetylglucosaminyltransferase-3, beta3gn-t7, beta1-3 n-acetylglucosaminyltransferase-1, poly-n-acetyllactosamine extension enzyme, udp-glcnac:betagal beta-1,3-n-acetylglucosaminyltransferase 1, beta1,3-n-acetylglucosaminyltransferase-7, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-
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B3gnt1
beta3GnT7
EC 2.4.1.163
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formerly
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Galbeta1-->4GlcNAc-R beta1-->3 N-acetylglucosaminyltransferase
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GnT-I
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i-GlcNAc transferase
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i-GlcNAcT
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IGnT
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N-acetyllactosamine beta(1-3)N-acetylglucosaminyltransferase
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poly-N-acetyllactosamine extension enzyme
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UDP-GlcNAc:Galbeta1-->4GlcNAcbeta-Rbeta1-->3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:Galbeta1-4Glc(NAc) beta-1,3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:GalR, beta-D-3-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-acetylglucosaminyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R 3-beta N-acetylglucosaminyltransferase (configuration-inverting)
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
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CAS REGISTRY NUMBER
COMMENTARY
85638-39-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
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115% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
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?
UDP-N-acetyl-alpha-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
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?
UDP-N-acetyl-D-glucosamine + asialo alpha1-acid glycoprotein
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sialic acid depleted alpha1-acid glycoprotein, 30% of the activity with neolactotetraosylceramide
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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i.e. neolactotetraosylceramide, GlcNAcT-2, synthesis in vitro of Ii core glycosphingolipids
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP-N-acetyl-D-glucosamine + Fucalpha(1->2)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)[Fucalpha(1->2)]Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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32% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-6)(Galbeta(1-4)GlcNAcbeta(1-2))Manalpha(1-6)Manbeta-octyl
?
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regioselectivity of enzyme, favored site of GlcNAc addition is the lower beta1,2-branch over the beta1,6-branch by a 3:1 ratio resulting in a mixture of heptasaccharides
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta-p-nitrophenol
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-p-nitrophenol
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
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48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
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76% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-pNP
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-pNP
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poor acceptor, 23% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
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48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
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101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
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28% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)Glcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)Glcbeta-O(CH2)3N3
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65% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAc
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
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73% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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107% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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90% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
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57% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
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101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
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104% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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100% activity
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
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26% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
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i.e. L2L4, 72% activity compared to L2L2
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?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
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i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate
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?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3Galbeta1,4(SO3-,6)-GlcNAc
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i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate
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?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
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mixture of the pentasaccharide product, 27 mol%, and the unreacted tetrasaccharide, 73 mol%
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
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?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OMe
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?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OR
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OR
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i.e. N,N-diacetyllactosediamine-OR
only 12.5% of the substrate is converted into the product, which is unusually resistant towards jackbean beta-N-acetylhexosaminidase
?
UDP-N-acetyl-D-glucosamine + glycolipid
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physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
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?
UDP-N-acetyl-D-glucosamine + keratan sulfate
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physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
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?
UDP-N-acetyl-D-glucosamine + lactosylceramide
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15% of the activity with neolactotetraosylceramide
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?
UDP-N-acetyl-D-glucosamine + O-glycan
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physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
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?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
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poly-N-acetyllactosamine is biosynthesized by the alternating addition of N-acetyl-D-glucosamine by UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase and Gal by UDP-Gal:betaGlcNAc beta-1,4-galactosyltransferase
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?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
UDP-N-acetylglucosamine + asialo-fetuin
UDP + N-acetylglucosaminylated asialo-fetuin
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much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
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?
UDP-N-acetylglucosamine + asialo-transferrin
UDP + N-acetylglucosaminylated asialo-transferrin
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much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
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?
UDP-N-acetylglucosamine + lactoneotetraosylceramide
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lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length
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?
UDP-N-acetylglucosamine + lactonorhexaosylceramide
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lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length
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?
UDP-N-acetylglucosamine + lactosylceramide
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lactosylceramide from human erythrocytes
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?
UDP-N-acetylglucosamine + lactotetraosylceramide
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lactotetraosylceramide from human meconium, poor substrate
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?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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-
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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involved in the biosynthesis of blood group precursors, enzyme might control the biosynthesis of the linear carbohydrate chain by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R structure present on oligosaccharides, glycosylceramides and glycoproteins
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
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-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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B3GNT1 displays an in vitro preference for the GlcNAcbeta1->2Man branch
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-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoproteins and other glycoproteins and oligosaccharides, GlcNAc residues are introduced to position C-3 of the terminal galactose of the glycoprotein
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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highly specific for acceptor oligosaccharides and glycoproteins carrying a terminal Galbeta(1-4)GlcNAcbeta1-R unit, catalyzes the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-R sequence, Galbeta(1-4)GlcNAcbeta(1-2)(Galbeta(1-4)GlcNAcbeta(1-6))Man pentasaccharide in the acceptor structure is a requirement for optimal activity, branch specificity, branches of this pentasaccharide structure, when contained in tri- and tetraantennary oligosaccharides, are highly preferred over other branches for attachment of the 1st and 2nd mol of GlcNAc into the acceptor molecule, enzyme also shows activity towards oligosaccharides related to blood group I- and i-active polylactosaminoglycans
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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might function in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group i antigenic structures, enzyme controls the synthesis of linear chain types by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R primer structure present on glycoprotein or glycolipid yielding a GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R sequence
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
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enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
i.e. lacto-N-neotetraose, 117% of activity with N-acetyllactosamine
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
?
UDP-N-acetyl-D-glucosamine + N-glycan
?
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
?
UDP-N-acetyl-D-glucosamine + N-glycan
?
-
polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
synthesis of N-acetyllactosamine repeats in Asn-linked oligosaccharides is enhanced by an increase of enzyme
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
enzyme is capable of elongation of polylactosamine i-chains
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
best acceptor among the glycoproteins that contain Galbeta(1-4)GlcNAc
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
GlcNAc residues are introduced to position C-3 of the terminal galactose of the glycoprotein, relative high activity towards asialo-alpha1-acid glycoprotein in Novikoff ascites tumor cells
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
much more effective than asialo-transferrin and asialo-fetuin, asialo-alpha1-acid glycoprotein from human plasma Cohn fraction V, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
-
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage, 51% of activity with N-acetyllactosamine
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
less physiological acceptor than N-acetyllactosamine
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
less effective acceptor than N-acetyllactosamine
-
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
70% of activity with N-acetyllactosamine
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
more physiological acceptor than lactose
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
more effective acceptor than lactose
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
more effective acceptor than lactose
-
?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
very poor substrate
-
-
?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure
-
-
?
additional information
?
-
-
terminal Galbeta(1-4)Glc(NAc) sequences, i.e. type II chains, are preferred substrates, no substrates: terminal Galbeta(1-3)GlcNAc sequences, i.e. type I chains, Lewis X trisaccharides, i.e. Galbeta(1-4)(Fucalpha(1-3))GlcNAc, monosaccharides, e.g. galactose, asialo-bovine submaxillary mucin that contains GalNAcalpha1-Ser/Thr and Galbeta(1-3)GalNAcalpha1-Ser/Thr
-
-
?
additional information
?
-
-
the enzyme shows no activity on a terminal Gal with Neu5Acalpha(2->6) substitution. Terminal galactose with alpha-linkage, Galalpha(1->3)Gal or Galalpha(1->4)Gal is not an acceptor substrate for the enzyme
-
-
?
additional information
?
-
-
no acceptors: melibiose, gentiobiose, galactose, glucose, N-acetylgalactosamine, N-acetylglucosamine
-
-
?
additional information
?
-
-
transfer of GlcNAc occurs mainly to type 2 chain nonfucosylated structures, elongation of type 1 chain structure Lc4 is also detectable, no transfer to any fucosylated derivative of either type 1 or 2 chains, transfer of GlcNAc to a terminal Gal residue on a lacto-series core chain
-
-
?
additional information
?
-
no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
?
additional information
?
-
-
biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia
-
-
?
additional information
?
-
-
the enzyme together with N-acetyllactosamine synthase, EC 2.4.1.90, catalyzes formation of linear glycans containing alternating beta-3-O-substituted residues of D-galactose and beta-4-O-substituted residues of N-acetyl-D-glucosamine, structures are present among others in glycosphingolipids or H-II type, polyglycosylceramides and polyglycosylpeptides, e.g. erythroglycan
-
-
?
additional information
?
-
-
enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line
-
-
?
additional information
?
-
-
substrate specificity, enzyme acts efficiently on keratan sulfate-related glycans, while lacto-N-tetraose and lacto-N-neo-tetraose are poor substrates, overview, product analysis
-
-
?
additional information
?
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
B3gnt1 null females are fertile, the B3gnt1 null males are not able to sire litters at the expected rate when mated to either wild type or B3gnt1-null females
-
-
?
additional information
?
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
asialo-serum glycoproteins are much more effective than O-glycoproteins as acceptors, overview over oligosaccharide substrates
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP-N-acetyl-D-glucosamine + glycolipid
?
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
?
UDP-N-acetyl-D-glucosamine + keratan sulfate
?
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
?
UDP-N-acetyl-D-glucosamine + O-glycan
?
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
involved in the biosynthesis of blood group precursors, enzyme might control the biosynthesis of the linear carbohydrate chain by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R structure present on oligosaccharides, glycosylceramides and glycoproteins
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
might function in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group i antigenic structures, enzyme controls the synthesis of linear chain types by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R primer structure present on glycoprotein or glycolipid yielding a GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R sequence
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
-
-
?
UDP-N-acetyl-D-glucosamine + N-glycan
?
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
?
UDP-N-acetyl-D-glucosamine + N-glycan
?
-
polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
synthesis of N-acetyllactosamine repeats in Asn-linked oligosaccharides is enhanced by an increase of enzyme
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
enzyme is capable of elongation of polylactosamine i-chains
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
-
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
-
-
?
additional information
?
-
-
biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia
-
-
?
additional information
?
-
-
the enzyme together with N-acetyllactosamine synthase, EC 2.4.1.90, catalyzes formation of linear glycans containing alternating beta-3-O-substituted residues of D-galactose and beta-4-O-substituted residues of N-acetyl-D-glucosamine, structures are present among others in glycosphingolipids or H-II type, polyglycosylceramides and polyglycosylpeptides, e.g. erythroglycan
-
-
?
additional information
?
-
-
enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line
-
-
?
additional information
?
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
B3gnt1 null females are fertile, the B3gnt1 null males are not able to sire litters at the expected rate when mated to either wild type or B3gnt1-null females
-
-
?
additional information
?
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Mg2+
Mn2+
additional information
Mn2+
-
optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+
Mn2+
-
requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+
additional information
-
enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+
additional information
-
absolute requirement for divalent cations, not stimulated by Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
nerve growth factor stimulated PC-12 cells with reduced GnT-i activity compared to unstimulated cells, no effect in PC-12D cells
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
detergent
-
activates, reaction rate is optimal at detergent:protein ratio of 1.6 in Triton CF-54 or X-100, 1:3 in Nonidet P-40
-
Triton CF-54
-
activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3%
additional information
-
CDPcholine stimulates by protecting UDP-GlcNAc from hydrolysis by endogenous enzymes
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma
B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma.
Adenocarcinoma of Lung
Identifying the prognostic significance of B3GNT3 with PD-L1 expression in lung adenocarcinoma.
Carcinogenesis
B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer.
Carcinoma
B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma.
Carcinoma, Non-Small-Cell Lung
B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.
Endometrial Neoplasms
B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway-associated markers.
Infections
B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.
Lung Neoplasms
B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.
Lung Neoplasms
B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer.
Lung Neoplasms
beta1,3-N-Acetylglucosaminyltransferase-7 (beta3Gn-T7) acts efficiently on keratan sulfate-related glycans.
Lymphatic Metastasis
B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.
Lymphatic Metastasis
B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.
Muscular Dystrophies
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of ?-dystroglycan.
Muscular Dystrophies
Integrative data mining highlights candidate genes for monogenic myopathies.
Neoplasm Metastasis
B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.
Neoplasm Metastasis
B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.
Neoplasm Metastasis
B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer.
Neoplasms
B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway-associated markers.
Neoplasms
B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.
Neoplasms
B3GNT3 expression suppresses cell migration and invasion and predicts favorable outcomes in neuroblastoma.
Neoplasms
B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.
Neoplasms
B3GNT3 overexpression promotes tumor progression and inhibits infiltration of CD8+ T cells in pancreatic cancer.
Neoplasms
B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer.
Neoplasms
B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma.
Neoplasms
Gene Variants That Affect Levels of Circulating Tumor Markers Increase Identification of Patients With Pancreatic Cancer.
Neoplasms
Identifying the prognostic significance of B3GNT3 with PD-L1 expression in lung adenocarcinoma.
Neoplasms
Novel role of O-glycosyltransferases GALNT3 and B3GNT3 in the self-renewal of pancreatic cancer stem cells.
Neoplasms
Tumor suppressor function of laminin-binding alpha-dystroglycan requires a distinct beta3-N-acetylglucosaminyltransferase.
Neuroblastoma
B3GNT3 expression suppresses cell migration and invasion and predicts favorable outcomes in neuroblastoma.
Pancreatic Neoplasms
B3GNT3 overexpression promotes tumor progression and inhibits infiltration of CD8+ T cells in pancreatic cancer.
Pancreatic Neoplasms
Novel role of O-glycosyltransferases GALNT3 and B3GNT3 in the self-renewal of pancreatic cancer stem cells.
Prostatic Neoplasms
Missense mutations in ?-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) cause Walker-Warburg syndrome.
Uterine Cervical Neoplasms
B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.
Walker-Warburg Syndrome
A truncating mutation in B3GNT1 causes severe Walker-Warburg syndrome.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE