Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.4.1.146 - beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase and Organism(s) Homo sapiens and UniProt Accession Q9Y2A9

for references in articles please use BRENDA:EC2.4.1.146
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q9Y2A9
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
=
+
3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
Synonyms
glcnact-ii, core 1 extension enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta1,3-N-acetylglucosaminyltransferase 3
-
core 1 extension beta1,3-N-acetylglucosaminyltransferase
-
core 1 extension enzyme
-
core1-beta3GlcNAcT
-
extended C1 beta3GnT3
-
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-mucin beta(1-3)- (elongating)
-
-
-
-
beta3GlcNAc-T (elongation)
-
-
elongation 3beta-GalNAc transferase
-
-
elongation 3beta-GalNAc-transferase
-
-
-
-
elongation beta3 Gn-T
-
-
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
-
-
-
-
uridine diphosphoacetylglucosamine-mucin beta(1-3)- (elongating) acetylglucosaminyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.
CAS REGISTRY NUMBER
COMMENTARY hide
87927-99-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
show the reaction diagram
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenyl
UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
show the reaction diagram
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
i.e. core class 1
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
not required
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000007
-
CaCo-2, colonic adenocarcinoma, cells differentiated to enterocytes
0.0000015
-
undifferentiated CaCo-2, colonic adenocarcinoma, cells
0.0000033
-
below, EBV immortilized B-cell line, from normal patients and such with the Wiskott-Aldrich syndrome
0.000006
-
normal granulocytes
0.000032
-
-
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
-
assay at
7
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high endothelial venule of secondary lymphoid organs
Manually annotated by BRENDA team
-
EBV immortilized B-cell line, from normal patients and such with the Wiskott-Aldrich syndrome
Manually annotated by BRENDA team
-
during progression to cancer
Manually annotated by BRENDA team
-
low level, in normal, but not in leukemic cells
Manually annotated by BRENDA team
additional information
-
not detectable in acute myeloid leukemia blast cell line and chronic myelogenous leukemia granulocyte cell line
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
O-glycan biosynthesis pathways and proposed O-glycan structures of selected glycosyltransferases, overview. Core 1 dominates the O-glycan repertoire. Core 1 can be further elongated by extended C1 beta3GnT3 enzyme to form extended core 1 structure, or be modified by C2 beta6GnT1 enzyme, EC 2.4.1.102, to form core 2 O-glycans. Regulation of O-glycan biosynthesis, overview. The extended C1 beta3GnT3 is competing withthe endogenous ST3Gal
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
B3GN3_HUMAN
372
1
42534
Swiss-Prot
Secretory Pathway (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
and monomer, PAGE and SDS-PAGE
monomer
-
and dimer, PAGE and SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
secreted protein is more heavily glycosylated and sialylated than its membrane-associated counterparts
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of a sequence fragment encoding the soluble catalytic domain, expression as His-tagged protein in CHO cells
gene B3GNT3, recombinant expression in CHO-K1 cells. Glycosylation-related genes in CHO-K1 cells are present, but strictly suppressed and regulated. CHO cells are transiently co-transfected with plasmids encoding extended C1 beta3GnT3, C2 beta6GnT1, or C3 beta3GnT6 with or without ST6Gal I or CHST4, respectively, and resulting O-glycan and core chain spectrum, overview
isolation and cloning of full length DNA via EST and expression in CHO cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
use of the recombinant enzyme for construction of the 6-sulfo sialyl Lewis x on extended core1 O-glycans
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Higgins, E.A.; Siminovitch, K.A.; Zhuang, D.; Brockhausen, I.; Dennis, J.W.
Aberrant O-linked oligosaccharide biosynthesis in lymphocytes and platelets from patients with the Wiskott-Aldrich syndrome
J. Biol. Chem.
266
6280-6290
1991
Homo sapiens
Manually annotated by BRENDA team
Brockhausen, I.; Kuhns, W.; Schachter, H.; Matta K.L.; Sutherland, D.R.; Baker, M.A.
Biosynthesis of O-glycans in leukocytes from normal donors and from patients with leukemia: increase in O-glycan core 2 UDP-GlcNAc:Galbeta3GalNAcalpha-R (GlcNAc to GalNAc) beta(1,6)-N-acetylglucosaminyltransferase in leikemic cells
Cancer Res.
51
1257-1263
1991
Homo sapiens
Manually annotated by BRENDA team
Brockhausen, I.; Romero, P.A.; Herscovics, A.
Glycosyltransferase changes upon differentiation of CaCo-2 human colonic adenocarcinoma cells
Cancer Res.
51
3136-3142
1991
Homo sapiens
Manually annotated by BRENDA team
Vavasseur, F.; Dole, K.; Yang, J.; Matta, K.L.; Myerscough, N.; Corfield, A.; Paraskeva, C.; Brockhausen, I.
O-glycan biosynthesis in human colorectal adenoma cells during progression to cancer
Eur. J. Biochem.
222
415-424
1994
Homo sapiens
Manually annotated by BRENDA team
Yeh, J.C.; Hiraoka, N.; Petryniak, B.; Nakayama, J.; Ellies, L.G.; Rabuka, D.; Hindsgaul, O.; Marth, J.D.; Lowe, J.B.; Fukuda, M.
Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta1,3-N-acetylglucosaminyltransferase
Cell
105
957-969
2001
Mus musculus, Homo sapiens (Q9Y2A9)
Manually annotated by BRENDA team
El-Battari, A.; Prorok, M.; Angata, K.; Mathieu, S.; Zerfaoui, M.; Ong, E.; Suzuki, M.; Lombardo, D.; Fukuda, M.
Different glycosyltransferases are differentially processed for secretion, dimerization, and autoglycosylation
Glycobiology
13
941-953
2003
Homo sapiens
Manually annotated by BRENDA team
Liu, J.; Jin, C.; Cherian, R.M.; Karlsson, N.G.; Holgersson, J.
O-glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O-glycan core enzyme cDNAs
J. Biotechnol.
199
77-89
2015
Homo sapiens (Q9Y2A9)
Manually annotated by BRENDA team