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EC Tree
IUBMB Comments Neither free phosphate nor maltose 1-phosphate is an intermediate in the reaction.
The expected taxonomic range for this enzyme is: Spinacia oleracea
Synonyms
synthase, maltose,
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synthase, maltose
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2 alpha-D-glucose 1-phosphate + H2O = maltose + 2 phosphate
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hexosyl group transfer
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alpha-D-glucose-1-phosphate:alpha-D-glucose-1-phosphate 4-alpha-D-glucosyltransferase (dephosphorylating)
Neither free phosphate nor maltose 1-phosphate is an intermediate in the reaction.
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alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
maltose + phosphate + phosphate
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additional information
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exchange of phosphate group of alpha-D-glucose 1-phosphate with phosphate is possible, neither maltose 1-phosphate nor free phosphate can be detected as an intermediate
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alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
maltose + phosphate + phosphate
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beta-D-glucose 1-phosphate
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D-glucono-1,5-lactone
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competitive
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1.5
alpha-D-glucose 1-phosphate
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additional information
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6.2 - 7.5
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30% of maximal activity at pH 6.2, 45% of maximal activity at pH 7.5
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spinach
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brenda
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brenda
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during purification addition of 15 mM alpha-D-glucose 1-phosphate to the isolation buffer is necessary for stabilization
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purification has to be carried out at 4°C
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Schilling, N.
Characterization of maltose biosynthesis from alpha-D-gluccose-1-phosphate in Spinacia oleracea L.
Planta
154
87-93
1982
Spinacia oleracea
brenda
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2.4.1.139 maltose synthase
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