Information on EC 2.4.1.139 - maltose synthase

for references in articles please use BRENDA:EC2.4.1.139
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The expected taxonomic range for this enzyme is: Spinacia oleracea

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EC NUMBER
COMMENTARY hide
2.4.1.139
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RECOMMENDED NAME
GeneOntology No.
maltose synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 alpha-D-glucose 1-phosphate + H2O = maltose + 2 phosphate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-glucose-1-phosphate:alpha-D-glucose-1-phosphate 4-alpha-D-glucosyltransferase (dephosphorylating)
Neither free phosphate nor maltose 1-phosphate is an intermediate in the reaction.
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CAS REGISTRY NUMBER
COMMENTARY hide
81669-74-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
spinach
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
maltose + phosphate + phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
-
exchange of phosphate group of alpha-D-glucose 1-phosphate with phosphate is possible, neither maltose 1-phosphate nor free phosphate can be detected as an intermediate
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
maltose + phosphate + phosphate
show the reaction diagram
-
-
-
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADPglucose
-
strong
beta-D-glucose 1-phosphate
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-
D-glucono-1,5-lactone
-
competitive
GDPglucose
-
strong
phosphate
-
-
UDPglucose
-
strong
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
alpha-D-glucose 1-phosphate
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 7.5
-
30% of maximal activity at pH 6.2, 45% of maximal activity at pH 7.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95000
-
gel filtration
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
during purification addition of 15 mM alpha-D-glucose 1-phosphate to the isolation buffer is necessary for stabilization
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purification has to be carried out at 4°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.139)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)