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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FX32

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FX32
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sucrose synthase, sucrose synthetase, mtsucs1, susy1, rsus3, sucrose synthase 1, psnsusy2, susy2, sucrose synthase 2, sucrose synthase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sucrose synthase
-
glucosyltransferase, uridine diphosphoglucose-fructose
-
-
-
-
sucrose synthase 1
-
sucrose synthase 2
-
sucrose synthetase
-
-
-
-
sucrose-UDP glucosyltransferase
-
-
-
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sucrose-uridine diphosphate glucosyltransferase
-
-
-
-
SUS6
-
isoform
SuSy3
-
isoform
SuSy4
-
isoform
SuSy5
-
isoform
SuSy6
-
isoform
UDP-glucose-fructose glucosyltransferase
-
-
-
-
UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
-
-
-
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uridine diphosphoglucose-fructose glucosyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
ADP + sucrose
ADP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
-
-
-
r
ADP-alpha-D-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
CDP + sucrose
CDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
CDP-alpha-D-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
dTDP + sucrose
dTDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
dTDP-alpha-D-glucose + D-fructose
dTDP + sucrose
show the reaction diagram
-
-
-
-
r
dUDP + sucrose
dUDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
dUDP-alpha-D-glucose + D-fructose
dUDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP + sucrose
GDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
GDP-alpha-D-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
the reaction is catalyzed at low pH values
-
-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
show the reaction diagram
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
-
-
r
UDP + sucrose
UDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
r
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
the reaction is catalyzed at low pH values
-
-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
show the reaction diagram
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
typical Michaelis-Menten behavior with respect to both UDP-glucose and D-fructose, no substrate inhibition at any UDP-glucose and D-fructose concentration tested
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017 - 0.17
ADP
4.37 - 42
D-fructose
31.58 - 185
sucrose
0.085 - 0.39
UDP
0.044 - 0.2
UDP-alpha-D-glucose
0.05 - 25
UDP-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
450
ADP
-
isoform SUS1, at pH 7.0 and 37°C
5.8 - 18.6
D-fructose
7.3 - 907
sucrose
7.3 - 907
UDP
5.8 - 18.6
UDP-alpha-D-glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
recombinant isoforms SUS1 and SUS3
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
about 10-15% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus. Within the silique wall, SUS localizes specifically to the companion cells, indicating that SUS activity may be required to provide energy for phloem transport activities in the silique wall
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
systemically affected sugar levels and enzyme activities in the shoots of the enzyme mutants, suggesting changes in the source-sink relationship. In sink tissues, sucrose is cleaved to make glucose and fructose available for energy-gaining reactions, macromolecule and amino acid biosynthesis. Heterodera schachtii root infection affects systemic sucrose processing
metabolism
the enzyme is involved in the metabolic regulation of the sucrose metabolism, overview
physiological function
malfunction
metabolism
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SUS6_ARATH
942
0
106876
Swiss-Prot
other Location (Reliability: 4)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
as a complex with UDP-glucose and as a complex with UDP and fructose, at 2.8- and 2.85 A resolution, respectively
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Agrobacterium tumefaciens
gene sus6, quantitative expression analysism, genotyping and phenotype-genotype relationship at different environmental conditions
recombinant overexpression in leaves of Nicotiana tabacum cv. SR1 plants via transfection with Agrobacterium tumefaciens strain GV3013
DNA and amino acid sequence determination and analysis, phylogenetic analysis
expressed in Escherichia coli BL21(DE3) cells
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expression in Agrobacterium tumefaciens
gene sus1, quantitative expression analysism, genotyping and phenotype-genotype relationship at different environmental conditions
gene sus2, quantitative expression analysism, genotyping and phenotype-genotype relationship at different environmental conditions
gene sus4, quantitative expression analysism, genotyping and phenotype-genotype relationship at different environmental conditions
gene sus5, quantitative expression analysism, genotyping and phenotype-genotype relationship at different environmental conditions
recombinant overexpression in leaves of Nicotiana tabacum cv. SR1 plants via transfection with Agrobacterium tumefaciens strain GV3013
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
effects of hypoxia, anoxia, and submergence on expression of genes sus6 in wild-type and in sus1, sus4, and sus1/sus4 mutants, overview
effects of hypoxia, anoxia, and submergence on expression of gene sus2 in wild-type and in sus1, sus4, and sus1/sus4 mutants, overview
effects of hypoxia, anoxia, and submergence on expression of gene sus3 in wild-type and in sus1, sus4, and sus1/sus4 mutants, overview
effects of hypoxia, anoxia, and submergence on expression of genes sus1 in wild-type and in sus1, sus4, and sus1/sus4 mutants, overview
effects of hypoxia, anoxia, and submergence on expression of genes sus4 in wild-type and in sus1, sus4, and sus1/sus4 mutants, overview
effects of hypoxia, anoxia, and submergence on expression of genes sus5 in wild-type and in sus1, sus4, and sus1/sus4 mutants, overview
transcript profiling shows that gene expression of AtSUS1 is upregulated in 3-day-old galls and laser-microdissected giant cells of Meloidogyne javanica
transcript profiling shows that gene expression of AtSUS4 is upregulated in 3-day-old galls and laser-microdissected giant cells of Meloidogyne javanica
under anoxia, the induction of SUS1 is stronger than that of SUS4
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fallahi, H.; Scofield, G.N.; Badger, M.R.; Chow, W.S.; Furbank, R.T.; Ruan, Y.L.
Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development
J. Exp. Bot.
59
3283-3295
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Arabidopsis thaliana (Q9FX32), Arabidopsis thaliana (Q9LXL5), Arabidopsis thaliana (Q9M111)
Manually annotated by BRENDA team
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Citrus unshiu (Q9SLY2), Citrus x paradisi, Coffea arabica (Q0E7D4), Daucus carota (O49845), Eucalyptus grandis (Q00P15), Eucalyptus grandis (Q00P16), Glycine max (P13708), Glycine max, Gossypium hirsutum (Q9XGB7), Gossypium hirsutum, Oryza sativa (P31924), Pisum sativum (O24301), Pisum sativum (O81610), Pisum sativum (Q9AVR8), Pisum sativum (Q9T0M9), Solanum lycopersicum (P49037), Solanum tuberosum (Q84T18), Vicia faba (P31926), Zea mays
Manually annotated by BRENDA team
Zheng, Y.; Anderson, S.; Zhang, Y.; Garavito, R.M.
The structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications
J. Biol. Chem.
286
36108-36118
2011
Arabidopsis thaliana (P49040), Arabidopsis thaliana
Manually annotated by BRENDA team
Almagro, G.; Baroja-Fernandez, E.; Munoz, F.J.; Bahaji, A.; Etxeberria, E.; Li, J.; Montero, M.; Hidalgo, M.; Sesma, M.T.; Pozueta-Romero, J.
No evidence for the occurrence of substrate inhibition of Arabidopsis thaliana sucrose synthase-1 (AtSUS1) by fructose and UDP-glucose
Plant Signal. Behav.
7
799-802
2012
Arabidopsis thaliana
Manually annotated by BRENDA team
Angeles-Nunez, J.G.; Tiessen, A.
Arabidopsis sucrose synthase 2 and 3 modulate metabolic homeostasis and direct carbon towards starch synthesis in developing seeds
Planta
232
701-718
2010
Arabidopsis thaliana (Q00917), Arabidopsis thaliana (Q9M111)
Manually annotated by BRENDA team
Baroja-Fernandez, E.; Munoz, F.J.; Li, J.; Bahaji, A.; Almagro, G.; Montero, M.; Etxeberria, E.; Hidalgo, M.; Sesma, M.T.; Pozueta-Romero, J.
Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production
Proc. Natl. Acad. Sci. USA
109
321-326
2012
Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q9M111)
Manually annotated by BRENDA team
Nguyen, Q.; Luan, S.; Wi, S.; Bae, H.; Lee, D.; Bae, H.
Pronounced phenotypic changes in transgenic tobacco plants overexpressing sucrose synthase may reveal a novel sugar signaling pathway
Front. Plant Sci.
6
1216
2016
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Arabidopsis thaliana (Q9FX32), Arabidopsis thaliana (Q9LXL5), Arabidopsis thaliana (Q9M111)
Manually annotated by BRENDA team
Cabello, S.; Lorenz, C.; Crespo, S.; Cabrera, J.; Ludwig, R.; Escobar, C.; Hofmann, J.
Altered sucrose synthase and invertase expression affects the local and systemic sugar metabolism of nematode-infected Arabidopsis thaliana plants
J. Exp. Bot.
65
201-212
2014
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Arabidopsis thaliana (Q9FX32), Arabidopsis thaliana (Q9LXL5), Arabidopsis thaliana (Q9M111), Arabidopsis thaliana
Manually annotated by BRENDA team
Santaniello, A.; Loreti, E.; Gonzali, S.; Novi, G.; Perata, P.
A reassessment of the role of sucrose synthase in the hypoxic sucrose-ethanol transition in Arabidopsis
Plant Cell Environ.
37
2294-2302
2014
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Arabidopsis thaliana (Q9FX32), Arabidopsis thaliana (Q9LXL5), Arabidopsis thaliana (Q9M111), Arabidopsis thaliana Col-0 (F4K5W8), Arabidopsis thaliana Col-0 (P49040), Arabidopsis thaliana Col-0 (Q00917), Arabidopsis thaliana Col-0 (Q9FX32), Arabidopsis thaliana Col-0 (Q9LXL5), Arabidopsis thaliana Col-0 (Q9M111)
Manually annotated by BRENDA team
Schmoelzer, K.; Gutmann, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
Sucrose synthase A unique glycosyltransferase for biocatalytic glycosylation process development
Biotechnol. Adv.
34
88-111
2016
Anabaena sp., Arabidopsis thaliana, Beta vulgaris, Vicia faba, Helianthus tuberosus, Hordeum vulgare, Ipomoea batatas, Manihot esculenta, Nitrosomonas europaea, Oryza sativa, Vigna radiata, Pisum sativum, Prunus persica, Pyrus pyrifolia, Saccharum sp., Solanum tuberosum, Zea mays, Thermosynechococcus vestitus, Acidithiobacillus caldus, Solanum chmielewskii, Glycine max (P13708)
Manually annotated by BRENDA team
Yao, D.; Gonzales-Vigil, E.; Mansfield, S.D.
Arabidopsis sucrose synthase localization indicates a primary role in sucrose translocation in phloem
J. Exp. Bot.
71
1858-1869
2020
Arabidopsis thaliana, Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917)
Manually annotated by BRENDA team