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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Vigna radiata var. radiata and UniProt Accession Q01390

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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Vigna radiata var. radiata
UNIPROT: Q01390
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Word Map
The taxonomic range for the selected organisms is: Vigna radiata var. radiata
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sucrose synthase, sucrose synthetase, susy1, mtsucs1, rsus3, sucrose synthase 1, psnsusy2, susy2, sucrose synthase 2, sucrose synthase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
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sucrose synthetase
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sucrose-UDP glucosyltransferase
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sucrose-uridine diphosphate glucosyltransferase
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Sus
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SuSy
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UDP-glucose-fructose glucosyltransferase
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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uridine diphosphoglucose-fructose glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NDP-alpha-D-glucose + D-fructose = NDP + sucrose
show the reaction diagram
mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
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-
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r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
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-
-
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r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
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-
-
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r
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
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-
-
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r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
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r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
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r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.44 - 0.9
CDP
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0.17 - 0.2
GDP
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16.9 - 40
sucrose
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cosubstrate ADP
0.3 - 0.38
UDPglucose
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cosubstrate TDP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SUS_VIGRR
805
0
92092
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
375000
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low speed sedimentation equilibrium centrifugation
405000
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gel filtration
94000
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4 * 94000, SDS-PAGE
additional information
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amino acid composition
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 94000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT, 0.1 mM, stabilizes
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EDTA, 0.1 mM, stabilizes
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freeze-thawing inactivates
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 0.1 mM EDTA and DTT, more than 50% of activity retained after 1 month
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Delmer, D.P.
The purification and properties of sucrose synthetase from etiolated Phaseolus aureus seedlings
J. Biol. Chem.
247
3822-3828
1972
Vigna radiata var. radiata
Manually annotated by BRENDA team