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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Glycine max and UniProt Accession P13708

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     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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This record set is specific for:
Glycine max
UNIPROT: P13708
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The taxonomic range for the selected organisms is: Glycine max
Synonyms
CaSUS1, CaSUS2, CsSUS3, glucosyltransferase, uridine diphosphoglucose-fructose, LjSUS3, Msus1, MtSucS1, mtSUS, PsnSuSy2, RSuS3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
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sucrose synthetase
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sucrose-UDP glucosyltransferase
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sucrose-uridine diphosphate glucosyltransferase
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Sus
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SuSy
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UDP-glucose-fructose glucosyltransferase
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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uridine diphosphoglucose-fructose glucosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
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?
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
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r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
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-
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r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
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-
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r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P13708
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
Ser11 is primary phosphorylation site, phosphorylation does not affect kinetics but partitioning between membrane and cytosol
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activation of sucrose synthesis; slight activation of sucrose cleavage at 10 mM
Mg2+
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activation; slight activation of sucrose cleavage
Mn2+
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activation
additional information
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no activation by K+, Na+, NH4+, Cl-, Br-, F-, NO3-, phosphate, sulfate, borate, acetate, citrate
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
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not inhibitory
ATP
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sucrose synthesis
fructose
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glucose
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imidazole
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Tris
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sucrose cleavage
UDP
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strong
UDPglucose
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UTP
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synthesis
additional information
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not inhibitory: DTT, GSH, 2-mercaptoethanol, EDTA; not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate; not inhibitory: galactose, mannose, maltose, raffinose, 3-phosphoglycerate, phosphoenolpyruvate, ethanol, succinate, 2-oxoglutarate, glutamine, NAD+, diphosphate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
allantoin
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activation, sucrose synthesis
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
ADP
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0.98 - 5
fructose
16.9 - 40
sucrose
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0.005
UDP
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0.012 - 0.033
UDPglucose
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15.1
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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sucrose cleavage
9.5
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sucrose synthesis
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.2
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about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage
7.5 - 10
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about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
SUS_SOYBN
805
0
92244
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
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4 * 90000, SDS-PAGE
380000
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SDS-PAGE
412000
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gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 90000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S11A
phosphorylation still occurs, but weakly
S11C
phosphorylation still occurs, but weakly
S11D
phosphorylation still occurs, but weakly
additional information
N-terminal truncation, phosphorylations still occurs, but weakly
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM potassium phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 4 weeks stable
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morell, M.; Copeland, L.
Sucrose synthase of soybean nodules
Plant Physiol.
78
149-154
1985
Glycine max
Manually annotated by BRENDA team
Thummler, F.; Verma, D.P.S.
Nodulin-100 of soybean is the subunit of sucrose synthase regulated by the availability of free heme in nodules
J. Biol. Chem.
262
14730-14736
1987
Glycine max
Manually annotated by BRENDA team
Zhang, X.Q.; Lund, A.A.; Sarath, G.; Cerny, R.L.; Roberts, D.M.; Chollet, R.
Soybean nodule sucrose synthase (nodulin-100): further analysis of its phosphorylation using recombinant and authentic root-nodule enzymes
Arch. Biochem. Biophys.
371
70-82
1999
Glycine max, Glycine max (P13708)
Manually annotated by BRENDA team
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Citrus unshiu (Q9SLY2), Citrus x paradisi, Coffea arabica (Q0E7D4), Daucus carota (O49845), Eucalyptus grandis (Q00P15), Eucalyptus grandis (Q00P16), Glycine max, Glycine max (P13708), Gossypium hirsutum, Gossypium hirsutum (Q9XGB7), Oryza sativa (P31924), Pisum sativum (O24301), Pisum sativum (O81610), Pisum sativum (Q9AVR8), Pisum sativum (Q9T0M9), Solanum lycopersicum (P49037), Solanum tuberosum (Q84T18), Vicia faba (P31926), Zea mays
Manually annotated by BRENDA team
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