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Information on EC 2.4.1.126 - hydroxycinnamate 4-beta-glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.126
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.126 hydroxycinnamate 4-beta-glucosyltransferase
IUBMB Comments
Acts on 4-coumarate, ferulate, caffeate and sinapate, forming a mixture of 4-glucosides and glucose esters (cf. EC 2.4.1.120 sinapate 1-glucosyltransferase).
The enzyme appears in viruses and cellular organisms
- 2.4.1.126
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calmodulin-binding
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heat-stable
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postmeiotic
- calmodulin
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footprint
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testis-specific
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calmodulin-dependent
Reaction Schemes
showSynonyms
glucosyltransferase, uridine diphosphoglucose-hydroxycinnamate, hydroxycinnamoyl glucosyltransferase, UDP-glucose-hydroxycinnamate glucosyltransferase, UDPglucose:trans-4-hydroxycinnamate 4-O-beta-D-glucosyltransferase, uridine diphosphoglucose-hydroxycinnamate glucosyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
glucosyltransferase, uridine diphosphoglucose-hydroxycinnamate
-
-
-
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hydroxycinnamoyl glucosyltransferase
-
-
-
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UDP-glucose-hydroxycinnamate glucosyltransferase
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-
-
-
UDPglucose:trans-4-hydroxycinnamate 4-O-beta-D-glucosyltransferase
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-
-
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uridine diphosphoglucose-hydroxycinnamate glucosyltransferase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + trans-4-hydroxycinnamate = UDP + 4-O-beta-D-glucosyl-4-hydroxycinnamate
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
daphnetin modification, esculetin modification
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-4-hydroxycinnamate 4-O-beta-D-glucosyltransferase
Acts on 4-coumarate, ferulate, caffeate and sinapate, forming a mixture of 4-glucosides and glucose esters (cf. EC 2.4.1.120 sinapate 1-glucosyltransferase).
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CAS REGISTRY NUMBER
COMMENTARY
77848-85-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 4-hydroxycinnamate
UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + sinapate
UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + sinapic acid
UDP + 4-O-beta-D-glucosyl-sinapic acid + 1-O-sinapoyl-beta-D-glucose
-
Substrates: -
Products: a mixture of the glucoside and the glucose ester is formed
Products: a mixture of the glucoside and the glucose ester is formed
?
UDPglucose + aesculin
UDP + 4-O-beta-D-glucosyl-aesculin
-
Substrates: -
Products: -
Products: -
?
UDPglucose + caffeic acid
UDP + 4-O-glucosyl-caffeic acid
-
Substrates: -
Products: no glucose ester is formed
Products: no glucose ester is formed
?
UDPglucose + daphnetin
UDP + 4-O-beta-D-glucosyl-daphnetin
-
Substrates: -
Products: -
Products: -
?
UDPglucose + ferulic acid
UDP + feruloyl-beta-D-glucose + 4-O-glucosyl-ferulic acid
-
Substrates: -
Products: a mixture of the glucoside and the glucose ester is formed
Products: a mixture of the glucoside and the glucose ester is formed
?
UDPglucose + kaempferol
UDP + 4-O-beta-D-glucosyl-kaempferol
-
Substrates: -
Products: -
Products: -
?
UDPglucose + p-coumaric acid
UDP + p-coumaroyl-beta-D-glucose + 4-O-glucosyl-p-coumaric acid
-
Substrates: -
Products: a mixture of the glucoside and the glucose ester is formed
Products: a mixture of the glucoside and the glucose ester is formed
?
UDPglucose + quercetin
UDP + 4-O-beta-D-glucosyl-quercetin
-
Substrates: -
Products: -
Products: -
?
UDPglucose + scopoletin
UDP + 4-O-beta-D-glucosyl-scopoletin
-
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 4-hydroxycinnamate
UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
Substrates: -
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
specific role for UGT84A3 in the accumulation of cell wall-bound 4-coumarate
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). U72E2_ARATH
481
0
52992
Swiss-Prot
other Location (Reliability: 3)
U72E3_ARATH
481
0
52904
Swiss-Prot
other Location (Reliability: 3)
A0A2P6S3Y3_ROSCH
124
0
14296
TrEMBL
Secretory Pathway (Reliability: 5)
A0A2P6S3Z5_ROSCH
85
0
9665
TrEMBL
other Location (Reliability: 3)
A0A396K2N6_MEDTR
395
0
44121
TrEMBL
Secretory Pathway (Reliability: 4)
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
beta-mercaptoethanol, 10 mM, stabilizes, 80% restorage of activity by its addition after treatment with p-chloromercuribenzoate
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, purified enzyme, 40% loss of activity after 24 h, 70% loss of activity after 48 h
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, compared to the wild type, crude protein extracts from leaves of UGT84A1 overexpression plants displayed increased activity toward 4-coumarate and ferulate, the preferred in vitro substrates, the overexpressing line UGT84A1 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A1, overexpression of UGT84A1 results in a significant increase of cell wall-bound sinapate
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, leaves of UGT84A3 overexpression displayed a significantly higher capacity to synthesize feruloylglucose, the overexpressing line UGT84A3 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A3, analysis of liberated from cell walls reveals for UGT84A3 a significant increase in cell wall-associated 4-coumarate, ferulate and sinapate
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, overexpression plants did not display changes in UGT specificity of leave extracts, the overexpressing line UGT84A4 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, UGT84A2 overexpression caused increasing activity toward sinapate, the overexpressing line UGT84A2 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A2, mature leaves of UGT84A2 show a slight increase in the sinapoylmalate content, overexpression of UGT84A2 results in a significant increase of cell wall-bound sinapate
given the impact of UGT84A3 overexpression on the amount of cell wall-associated hydroxycinnamates, analysis of the cell wall fraction of the insertion knock-out mutant UGT84A3 shows a significant decrease of the 4-coumarate content in cell walls of null mutant whereas the levels of ferulate and sinapate are not changed compared to the wild type
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fleuriet, A.; Macheix, J.J.
Partial purification and some properties of a hydroxycinnamoyl glucosyltransferase from tomato fruits
Z. Naturforsch. C
35
967-972
1980
Solanum lycopersicum
-
brenda
Meissner, D.; Albert, A.; Boettcher, C.; Strack, D.; Milkowski, C.
The role of UDP-glucose:hydroxycinnamate glucosyltransferases in phenylpropanoid metabolism and the response to UV-B radiation in Arabidopsis thaliana
Planta
228
663-674
2008
Arabidopsis thaliana (O23402)
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.126)
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Release 2023.1 (January 2023)
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