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Information on EC 2.4.1.109 - dolichyl-phosphate-mannose-protein mannosyltransferase and Organism(s) Rattus norvegicus and UniProt Accession Q99PR0

for references in articles please use BRENDA:EC2.4.1.109
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EC Tree
IUBMB Comments
The enzyme transfers mannosyl residues to the hydroxy group of serine or threonine residues, producing cell-wall mannoproteins. It acts only on long-chain alpha-dihydropolyprenyl derivatives, larger than C35.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q99PR0
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pomt1, pomt2, protein o-mannosyltransferase, pmt1p, o-mannosyltransferase, protein o-mannosyltransferase 1, pmt2p, pmt4p, pmt1p-pmt2p, aapmta, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dolichyl-phosphate-mannose-protein mannosyltransferase 1
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protein O-mannosyl-transferase 1
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dolichol phosphomannose-protein mannosyltransferase
-
-
-
-
dolichyl-phosphate-mannose-protein mannosyltransferase 2
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mannosyltransferase, dolichol phosphomannose-protein
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-
-
-
protein O-D-mannosyltransferase
-
-
-
-
protein O-mannosyl-transferase 2
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dolichyl-phosphate-D-mannose:protein O-D-mannosyltransferase
The enzyme transfers mannosyl residues to the hydroxy group of serine or threonine residues, producing cell-wall mannoproteins. It acts only on long-chain alpha-dihydropolyprenyl derivatives, larger than C35.
CAS REGISTRY NUMBER
COMMENTARY hide
74315-99-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dolichyl phosphate D-mannose + human ribonuclease 2
dolichyl phosphate + human ribonuclease 2-D-mannose
show the reaction diagram
dolichyl phosphate D-mannose + ribonuclease 2
dolichyl phosphate + ribonuclease 2-D-mannose
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dolichyl phosphate D-mannose + ribonuclease 2
dolichyl phosphate + ribonuclease 2-D-mannose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chymotrypsin
-
inactivation
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Pomt1 and Pomt2 mRNA are coexpressed in neurons (dentate gyrus and CA1-CA3 region of the hippocampus and cerebellar Purkinje cells)
Manually annotated by BRENDA team
Pomt1 is most strongly expressed in testis
Manually annotated by BRENDA team
Pomt1 and Pomt2 mRNA are coexpressed in neurons (dentate gyrus and CA1-CA3 region of the hippocampus and cerebellar Purkinje cells)
Manually annotated by BRENDA team
Pomt2 is most strongly expressed in testis
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
POMT1_RAT
747
10
85496
Swiss-Prot
Secretory Pathway (Reliability: 5)
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26
-
22 h, stable
37
-
60 min, stable
95
-
inactivation
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Doucey, M.A.; Hess, D.; Cacan, R.; Hofsteenge, J.
Protein C-mannosylation is enzyme-catalysed and uses dolichyl-phosphate-mannose as a precursor
Mol. Biol. Cell
9
291-300
1998
Rattus norvegicus
Manually annotated by BRENDA team
Manya, H.; Chiba, A.; Margolis, R.U.; Endo, T.
Molecular cloning and characterization of rat Pomt1 and Pomt2
Glycobiology
16
863-873
2006
Rattus norvegicus (Q14U74), Rattus norvegicus (Q99PR0)
Manually annotated by BRENDA team