Information on EC 2.4.1.104 - o-dihydroxycoumarin 7-O-glucosyltransferase

for references in articles please use BRENDA:EC2.4.1.104
Word Map on EC 2.4.1.104
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Nicotiana tabacum

top print hide
EC NUMBER
COMMENTARY hide
2.4.1.104
-
top print hide
RECOMMENDED NAME
GeneOntology No.
o-dihydroxycoumarin 7-O-glucosyltransferase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 7,8-dihydroxycoumarin = UDP + daphnin
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cichoriin interconversion
-
-
daphnin interconversion
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:7,8-dihydroxycoumarin 7-O-beta-D-glucosyltransferase
Converts the aglycone daphetin into daphnin and, more slowly, esculetin into cichoriin, umbelliferone into skimmin, hydrangetin into hydrangin and scopoletin into scopolin.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
74114-37-7
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-glucose + 4-coumaric acid
UDP + 4-O-glucosylcoumaric acid
show the reaction diagram
-
-
-
-
?
UDP-glucose + 4-methylumbelliferone
UDP + 7-O-glucosyl-4-methylumbelliferone
show the reaction diagram
-
-
-
-
?
UDP-glucose + 7,8-dihydroxycoumarin
UDP + daphnin
show the reaction diagram
UDP-glucose + baicalein
UDP + 7-O-glucosylbaicalein
show the reaction diagram
-
-
-
-
?
UDP-glucose + caffeic acid
UDP + ?
show the reaction diagram
UDP-glucose + esculetin
UDP + cichoriin
show the reaction diagram
UDP-glucose + ferulic acid
UDP + 4-O-glucosylferulic acid
show the reaction diagram
-
low activity
-
-
?
UDP-glucose + formononetin
UDP + 7-O-glucosylformononetin
show the reaction diagram
-
-
-
-
?
UDP-glucose + hydrangetin
UDP + hydrangin
show the reaction diagram
UDP-glucose + protocatechuic acid
UDP + ?
show the reaction diagram
-
12% of the activity with 7,8-dihydroxycoumarin
-
-
?
UDP-glucose + scopoletin
UDP + scopolin
show the reaction diagram
UDP-glucose + syringic acid
UDP + 4-O-glucosyl syringic acid
show the reaction diagram
-
10% of the activity with 7,8-dihydroxycoumarin
-
-
?
UDP-glucose + umbelliferone
UDP + skimmin
show the reaction diagram
UDP-glucose + vanillic acid
UDP + 4-O-glucosylvanillic acid
show the reaction diagram
-
14% of the activity with 7,8-dihydroxycoumarin
-
-
?
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 7,8-dihydroxycoumarin
UDP + daphnin
show the reaction diagram
-
i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin
-
?
UDP-glucose + esculetin
UDP + cichoriin
show the reaction diagram
-
i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin
-
?
UDP-glucose + scopoletin
UDP + scopolin
show the reaction diagram
-
i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin
-
?
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
1 mM, activation to 114% of control
Cu2+
-
1 mM, activation to 112% of control
Mn2+
-
1 mM, activation to 133% of control
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
1 mM, 87% inhibition
Cu2+
-
1 mM, 55% inhibition
Zn2+
-
1 mM, 96% inhibition
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
10 mM, activation to 141% of control
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.095
daphnetin
-
-
0.025 - 0.11
esculetin
0.15 - 1.43
scopoletin
0.043 - 0.05
UDPglucose
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000 - 50000
-
gel filtration
49000
-
1 * 49000, SDS-PAGE
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 49000, SDS-PAGE
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.104)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)