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Information on EC 2.4.1.10 - levansucrase and Organism(s) Rahnella aquatilis and UniProt Accession O54435

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.10 levansucrase
IUBMB Comments
Some other sugars can act as D-fructosyl acceptors.
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This record set is specific for:
Rahnella aquatilis
UNIPROT: O54435
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The taxonomic range for the selected organisms is: Rahnella aquatilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
levansucrase, 6-sft, sucrose:fructan 6-fructosyltransferase, fructansucrase, lsc-3, t2-ls, t1-ls, sucrose 6-fructosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-2,6-fructan:D-glucose 1-fructosyltransferase
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-
-
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beta-2,6-fructosyltransferase
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-
-
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fructosyltransferase, sucrose 6-
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-
-
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sucrose 6-fructosyltransferase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
show the reaction diagram
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
sucrose:[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside 6-beta-D-fructosyltransferase
Some other sugars can act as D-fructosyl acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-17-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + sucrose
D-glucose + levan
show the reaction diagram
the recombinant enzyme shows 80% conversion yield
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
show the reaction diagram
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + cellobiose
?
show the reaction diagram
-
-
-
-
?
sucrose + D-arabinose
?
show the reaction diagram
-
-
-
-
?
sucrose + D-maltose
?
show the reaction diagram
-
-
-
-
?
sucrose + D-sorbitol
?
show the reaction diagram
-
-
-
-
?
sucrose + D-xylose
?
show the reaction diagram
-
-
-
-
?
sucrose + L-arabinose
?
show the reaction diagram
-
-
-
-
?
sucrose + lactose
?
show the reaction diagram
-
-
-
-
?
sucrose + lactose
lactosucrose
show the reaction diagram
-
-
-
-
?
sucrose + maltotriose
?
show the reaction diagram
-
-
-
-
?
sucrose + melibiose
?
show the reaction diagram
-
-
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-
?
sucrose + methanol
glucose + methyl-beta-D-fructoside
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Iodine
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slight inhibition
Ni2+
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slight inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50
sucrose
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
101.2
crude enzyme, at pH 6.0 and 37°C
195.3
after 1.93fold purification, at pH 6.0 and 37°C
731
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
recombinant enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
about 85% activity at pH 5.0, 100% activity at pH 6.0, about 70% activity at pH 7.0
3 - 10
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almost no activity under pH 3.0 and above pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
levan production
40
levan formation
50
maximal reaction velocity
55 - 60
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sucrose + D-xylose
additional information
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
about 88% activity 20°C, about 100% activity at 30-40°C, about 30% activity 50°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
recombinant enzyme expressed in Escherichia coli
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
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gel filtration
49000
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x * 49000, recombinant enzyme, SDS-PAGE
64000
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2 * 64000, SDS-PAGE
additional information
amino acid sequence alignment
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 47000, SDS-PAGE
?
-
x * 49000, recombinant enzyme, SDS-PAGE
dimer
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2 * 64000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
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30°C, 2 h, stable
488313
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
20 min, stable below
60
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20 min, 50% loss of activity
70
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20 min, complete loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography
recombinant from E. coli as His-tagged protein
recombinant overexpression in E. coli, His-tagged protein
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain Y2805gs
expression of His-tagged enzyme in Escherichia coli DH5alpha
overexpression of His-tagged enzyme in Escherichia coli, gene lscR
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
enzyme is an important biocatalyst for production of fructose homopolymers, potential usage in bioindustrial fields
synthesis
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production of the artificial sweetener, lactosucrose
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohtsuka, K.; Hino, S.; Fukushima, T.; Ozawa, O.; Kanematsu, T.; Uchida, T.
Characterization of levansucrase from Rahnella aquatilis JCM-1683
Biosci. Biotechnol. Biochem.
56
1373-1377
1992
Rahnella aquatilis, Rahnella aquatilis JCM -1683
-
Manually annotated by BRENDA team
Kim, H.; Park, H.E.; Kim, M.J.; Lee, H.G.; Yang, J.Y.; Cha, J.
Enzymatic characterization of a recombinant levansucrase from Rahnella aquatilis ATCC 15552
J. Microbiol. Biotechnol.
13
230-235
2003
Rahnella aquatilis, Rahnella aquatilis ATCC 15552
-
Manually annotated by BRENDA team
Gon Kim, M.; Ho Kim, C.; Sik Lee, J.; Bang Song, K.; Ki Rhee, S.
Synthesis of methyl beta-D-fructoside catalyzed by levansucrase from Rahnella aquatilis
Enzyme Microb. Technol.
27
646-651
2000
Rahnella aquatilis
Manually annotated by BRENDA team
Song, K.B.; Seo, J.W.; Kim, M.G.; Rhee, S.K.
Levansucrase of Rahnella aquatilis ATCC33071: gene cloning, expression, and levan formation
Ann. N. Y. Acad. Sci.
864
506-511
1998
Zymomonas mobilis, Rahnella aquatilis (O54435), Rahnella aquatilis ATCC 33071 (O54435)
Manually annotated by BRENDA team
Park, N.H.; Choi, H.J.; Oh, D.K.
Lactosucrose production by various microorganisms harboring levansucrase activity
Biotechnol. Lett.
27
495-497
2005
Geobacillus stearothermophilus, Bacillus amyloliquefaciens, Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas syringae, Rahnella aquatilis, Sterigmatomyces elviae, Sterigmatomyces elviae ATCC 18894, Geobacillus stearothermophilus ATCC 12980, Pseudomonas syringae IFO14086, Bacillus amyloliquefaciens IFO15535, Rahnella aquatilis KCTC2858, Paenibacillus polymyxa KCCM35411
Manually annotated by BRENDA team
Ko, H.; Bae, J.H.; Sung, B.H.; Kim, M.J.; Kim, C.H.; Oh, B.R.; Sohn, J.H.
Efficient production of levan using a recombinant yeast Saccharomyces cerevisiae hypersecreting a bacterial levansucrase
J. Ind. Microbiol. Biotechnol.
46
1611-1620
2019
Rahnella aquatilis (O54435), Rahnella aquatilis, Rahnella aquatilis ATCC 33071 (O54435)
Manually annotated by BRENDA team