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Information on EC 2.3.3.5 - 2-methylcitrate synthase and Organism(s) Corynebacterium glutamicum and UniProt Accession Q8NSH7

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.5 2-methylcitrate synthase
IUBMB Comments
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC 2.3.3.1, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.
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Corynebacterium glutamicum
UNIPROT: Q8NSH7
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Word Map
The taxonomic range for the selected organisms is: Corynebacterium glutamicum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
methylcitrate synthase, 2-methylcitrate synthase, prpc2, 2-mcs, citrate synthase ii, 2-mcs1, rv1131, 2-mc synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-methylcitrate oxaloacetate-lyase
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-
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MCA condensing enzyme
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MCS
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-
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Methylcitrate synthase
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methylcitrate synthetase
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
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-
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming)
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC 2.3.3.1, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.
CAS REGISTRY NUMBER
COMMENTARY hide
57827-78-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 13032, prp gene cluster
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42570
prpC1, amino acid sequence calculated from cDNA
42600
prpC2, amino acid sequence calculated from cDNA
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
genome sequencing, prpDBC gene cluster, prpC2 gene for 2-methylcitrate synthase, cloned into vector pTrc99a and expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Claes, W.A.; Puehler, A.; Kalinowski, J.
Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle
J. Bacteriol.
184
2728-2739
2002
Aspergillus nidulans, Corynebacterium glutamicum, Corynebacterium glutamicum (Q8NSH7), Corynebacterium glutamicum (Q8NSL1), Cupriavidus metallidurans, Cupriavidus metallidurans (Q937N9), Escherichia coli, Escherichia coli (P31660), Paraburkholderia sacchari, Pseudomonas aeruginosa, Salmonella enterica (Q56063), Salmonella enterica, Yarrowia lipolytica
Manually annotated by BRENDA team