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Information on EC 2.3.3.16 - citrate synthase (unknown stereospecificity) and Organism(s) Sus scrofa and UniProt Accession P00889

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EC Tree
IUBMB Comments
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
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This record set is specific for:
Sus scrofa
UNIPROT: P00889
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The taxonomic range for the selected organisms is: Sus scrofa
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
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acetyl-CoA
+
H2O
+
oxaloacetate
=
citrate
+
CoA
+
+
=
+
Synonyms
citrate synthetase, mitochondrial citrate synthase, peroxisomal citrate synthase, si-citrate synthase, type ii citrate synthase, bifunctional citrate synthase/2-methylcitrate synthase, citrate condensing enzyme, citrate synthase cit1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
citrate synthase
-
citrate condensing enzyme
-
-
-
-
citrate oxaloacetate-lyase (CoA-acetylating)
-
-
-
-
citrate oxaloacetate-lyase, CoA-acetylating
-
-
-
-
citrate synthase
-
-
-
-
citrate synthetase
-
-
-
-
citric synthase
-
-
-
-
citric-condensing enzyme
-
-
-
-
citrogenase
-
-
-
-
oxalacetic transacetase
-
-
-
-
oxaloacetate transacetase
-
-
-
-
synthase, citrate
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
show the reaction diagram
active site
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
of intermediate citryl-CoA
Claisen condensation
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
CAS REGISTRY NUMBER
COMMENTARY hide
9027-96-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
show the reaction diagram
-
condensation step is reversible
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
?
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
167
acetyl-CoA
-
pH 7.5
117
citrate
-
-
additional information
additional information
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CISY_PIG
464
0
51629
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
pH 7.8, PEG 3350, aspartame, benzamidine, cysteamine
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
commercial preparation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kurz, L.C.; Drysdale, G.; Riley, M.; Tomar, M.A.; Chen, J.; Russell, R.J.M.; Danson, M.J.
Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum
Biochemistry
39
2283-2296
2000
Sus scrofa, Thermoplasma acidophilum
Manually annotated by BRENDA team
Larson, S.B.; Day, J.S.; Nguyen, C.; Cudney, R.; McPherson, A.
Structure of pig heart citrate synthase at 1.78 A resolution
Acta Crystallogr. Sect. F
65
430-434
2009
Sus scrofa (P00889)
Manually annotated by BRENDA team
Rozycki, B.; Cieplak, M.
Citrate synthase proteins in extremophilic organisms: studies within a structure-based model
J. Chem. Phys.
141
235102
2014
Sus scrofa (P00889), Pyrococcus furiosus (Q53554), Pyrobaculum aerophilum (Q8ZWP2)
Manually annotated by BRENDA team