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Information on EC 2.3.3.10 - hydroxymethylglutaryl-CoA synthase and Organism(s) Mus musculus and UniProt Accession P54869

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Mus musculus
UNIPROT: P54869 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hmg-coa, hmg-coa synthase, hmgcs2, hmgs, hmgcs1, 3-hydroxy-3-methylglutaryl-coa synthase, hydroxymethylglutaryl-coa synthase, 3-hydroxy-3-methylglutaryl-coenzyme a synthase, 3-hydroxy-3-methylglutaryl coenzyme a synthase, hmgcs, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxy-3-methylglutaryl-CoA synthase 2
-
HMG-CoA synthase
-
(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating)
-
-
-
-
3-hydroxy-3-methylglutaryl CoA synthetase
-
-
-
-
3-hydroxy-3-methylglutaryl coenzyme A synthase
-
-
-
-
3-hydroxy-3-methylglutaryl coenzyme A synthetase
-
-
-
-
3-hydroxy-3-methylglutaryl-CoA synthase
-
-
-
-
3-hydroxy-3-methylglutaryl-coenzyme A synthase
-
-
-
-
acetoacetyl coenzyme A transacetase
-
-
-
-
beta-hydroxy-beta-methylglutaryl-CoA synthase
-
-
-
-
HMG-CoA synthase
-
-
-
-
hydroxymethylglutaryl CoA synthetase
-
-
-
-
hydroxymethylglutaryl coenzyme A synthase
-
-
-
-
hydroxymethylglutaryl coenzyme A-condensing enzyme
-
-
-
-
hydroxymethylglutaryl-coenzyme A synthase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
condensation
-
-
-
-
hydrolysis
-
-
-
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Acyl group transfer
-
-
-
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transacetylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:acetoacetyl-CoA C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-44-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
ir
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
ir
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.035
activity in response to acetaminophen, APAP, treatment
0.057
control, no acetaminophen, APAP, treatment
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
activity assay
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
cholesterogenic isoform
Manually annotated by BRENDA team
cholesterogenic isoform
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HMCS2_MOUSE
508
0
56823
Swiss-Prot
Mitochondrion (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56800
determined by MALDI-TOF mass spectrometry
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
mitochondria are prepared
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
3-hydroxy-3-methylglutaryl-CoA synthase 2 has significantly decreased levels of reduced thiols and activity after acetaminophen treatment
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
acetaminophen, APAP, is a leading cause of drug-induced liver failure
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Greenspan, M.D.; Bull, H.B.; Yudkovitz, J.B.; Hanf, D.P.; Alberts, A.W.
Inhibition of 3-hydroxy-3-methylglutaryl-CoA synthase and cholesterol biosynthesis by beta-lactone inhibitors and binding of these inhibitors to the enzyme
Biochem. J.
289
889-895
1993
Mesocricetus auratus, Mus musculus, Rattus norvegicus
-
Manually annotated by BRENDA team
Misra, I.; Charlier, H.A.; Miziorko, H.M.
Avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase. Evaluation of the role of cysteines in reaction chemistry
Biochim. Biophys. Acta
1247
253-259
1995
Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus, Cricetulus griseus (P13704), Blattella germanica (P54870), Blattella germanica (P54961)
Manually annotated by BRENDA team
Misra, I.; Miziorko, H.M.
Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase H264 with acetoacetyl-CoA
Biochemistry
35
9610-9616
1996
Bos taurus, Saccharomyces cerevisiae, Gallus gallus, Homo sapiens, Mesocricetus auratus, Mus musculus, Rattus norvegicus, Blattella germanica (P54870), Blattella germanica (P54961)
Manually annotated by BRENDA team
Chun, K.Y.; Vinarov, D.A.; Miziorko, H.M.
3-Hydroxy-3-methylglutaryl-CoA synthase: Participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate
Biochemistry
39
14670-14681
2000
Saccharomyces cerevisiae, Saccharomyces cerevisiae (P54839), Gallus gallus, Gallus gallus (P23228), Homo sapiens, Homo sapiens (P54868), Homo sapiens (Q01581), Mesocricetus auratus, Methanothermobacter thermautotrophicus, Sus scrofa (O02734), Borreliella burgdorferi (O51626), Cricetulus griseus (P13704), Rattus norvegicus (P17425), Rattus norvegicus (P22791), Mus musculus (P54869), Blattella germanica (P54870), Blattella germanica (P54961), Caenorhabditis elegans (P54871), Arabidopsis thaliana (P54873), Schizosaccharomyces pombe (P54874), Pinus sylvestris (P93773)
Manually annotated by BRENDA team
Chun, K.Y.; Vinarov, D.A.; Zajicek, J.; Miziorko, H.M.
3-hydroxy-3-methylglutaryl-CoA synthase. A role for glutamate 95 in general acid/base catalysis of C-C bond formation
J. Biol. Chem.
275
17946-17953
2000
Gallus gallus, Gallus gallus (P23228), Homo sapiens, Homo sapiens (P54868), Methanothermobacter thermautotrophicus, Sus scrofa (O02734), Borreliella burgdorferi (O51626), Rattus norvegicus (P22791), Mus musculus (P54869), Blattella germanica (P54870), Blattella germanica (P54961), Arabidopsis thaliana (P54873), Schizosaccharomyces pombe (P54874), Pinus sylvestris (P93773)
Manually annotated by BRENDA team
Sutherlin, A.; Hedl, M.; Sanchez-Neri, B.; Burgner, J.W.; Stauffacher, C.V.; Rodwell, V.W.
Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis
J. Bacteriol.
184
4065-4070
2002
Blattella germanica, Borreliella burgdorferi, Bos taurus, Streptomyces sp., Gallus gallus, Streptococcus pneumoniae, Enterococcus faecalis, Homo sapiens, Mesocricetus auratus, Staphylococcus aureus, Mus musculus, Rattus norvegicus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Streptococcus pyogenes, Sus scrofa
Manually annotated by BRENDA team
Andringa, K.K.; Bajt, M.L.; Jaeschke, H.; Bailey, S.M.
Mitochondrial protein thiol modifications in acetaminophen hepatotoxicity: effect on HMG-CoA synthase
Toxicol. Lett.
177
188-197
2008
Mus musculus (P54869)
Manually annotated by BRENDA team