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Information on EC 2.3.2.B8 - E2:E3 ubiquitin transferase
for references in articles please use BRENDA:EC2.3.2.B8
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preliminary BRENDA-supplied EC number
EC Tree 2 Transferases
2.3 Acyltransferases
2.3.2 Aminoacyltransferases
2.3.2.B8 E2:E3 ubiquitin transferase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine
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[HECT-E3-ubiquitin-carrier protein]-L-cysteine
=
[ubiquitin-carrier protein E2]-L-cysteine
+
S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
Synonyms
e3 ubiquitin-protein ligase, ubch5, e3 hect, ubcm3, e3 ubiquitin-protein ligase itchy homolog, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
E3 ubiquitin-protein ligase Itchy homolog
HECT E3 ligase
Itch
UBE2E1
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine = [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine = [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
the 40 kDa C-terminal HET domain binds a reactive thioester-linked E2-ubiquitin covalent-linked complex. The ubiqiutin is transferred from the E2 catalytic cysteine to the HECT domain catalytic cysteine via a transthiolation reaction
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine = [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
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SYSTEMATIC NAME
IUBMB Comments
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine: [E3 acceptor protein] ubiquitin transferase
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein cIAP2]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein cIAP2]-L-cysteine
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S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein UbcH5]-S-ubiquitinyl-L-cysteine + [HECT-E3-ubiquitin-carrier protein E6-AP]-L-cysteine
[ubiquitin-carrier protein UbcH5]-L-cysteine + [HECT-E3-ubiquitin-carrier protein E6-AP]-S-ubiquitinyl-L-cysteine
Substrates: a region of isoform UbcH5 encompassing the catalytic site cysteine residue is critical for its ability to interact with E3 enzymes E6-AP and RSP5. Of particular importance is a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2s
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[ubiquitin-carrier protein UbcH5]-S-ubiquitinyl-L-cysteine + [HECT-E3-ubiquitin-carrier protein Rsp5]-L-cysteine
[ubiquitin-carrier protein UbcH5]-L-cysteine + [HECT-E3-ubiquitin-carrier protein Rsp5]-S-ubiquitinyl-L-cysteine
Substrates: a region of isoform UbcH5 encompassing the catalytic site cysteine residue is critical for its ability to interact with E3 enzymes E6-AP and RSP5. Of particular importance is a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2s
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S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
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S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
Substrates: -
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S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
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additional information
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Substrates: the full-length UBE2E proteins primarily promote protein monoubiquitylation, whereas the core UBC domain of each can efficiently build polyubiquitin chains
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Substrates: the N-terminal extension of Ube2E1 undergoes intramolecular auto-ubiquitination. This self-ubiquitination activity is enhanced in the presence of interacting RING E3 ligases like RNF4 and results in a progressive attenuation of the E2 activity toward substrate/E3 modification
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Substrates: corresponding to Cys942 of HECT domain of NEDD4L, Cys890, Cys838, Cys1574, and Cys1540 residues of the HECT domains of WWP1, WWP2, HECW1, and HECW2, respectively, are involved in E2-to-E3 ubiquitin transfer
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Substrates: the HECT-type E3 ligase TRIP12 promotes pro-interleukin-1beta ubiquitinylation (on lysine residues mainly in the pro-domain with K27-, K29- and K33- polyubiquitin chains) and proteasomal turnover and reduce mature interleukin-1beta production
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Substrates: the enzyme is autoinhibited by an intramolecular interaction between its HECT (homologous to the E6-AP carboxy terminus domain) and two central WW domains. Ndfip1 binds these WW domains to release the HECT, allowing trans-thiolation and enzyme catalytic activity
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Substrates: the HECT-type E3 ligase AREL1 promotes pro-interleukin-1beta ubiquitinylation (on lysine residues mainly in the pro-domain with K27-, K29- and K33- polyubiquitin chains) and proteasomal turnover and reduce mature interleukin-1beta production
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
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Substrates: -
Products: -
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?
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
Substrates: -
Products: -
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?
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
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Substrates: -
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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two E2UbcH5B-derived peptides (His55-Phe69 and Asn81-Ala96) show binding abilities against the HECT domains of NEDD4L, WWP1, WWP2, HECW1, and HECW2. Substitution of Phe62, Pro65, Ile84, and Cys85 residues into Ala reveal an impaired binding, suggesting that the proposed peptide ligands may selectively target E2-HECT binding and ubiquitin transfer
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma
UBCH5 Family Members Differentially Impact Stabilization of Mutant p53 via RNF128 Iso1 During Barrett's Progression to Esophageal Adenocarcinoma.
Amyotrophic Lateral Sclerosis
Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
Angelman Syndrome
Tissue-specific variation of Ube3a protein expression in rodents and in a mouse model of Angelman syndrome.
Autoimmune Diseases
A sesquiterpene lactone from a medicinal herb inhibits proinflammatory activity of TNF-? by inhibiting ubiquitin-conjugating enzyme UbcH5.
Autoimmune Diseases
E3 ubiquitin-protein ligase TRIM21-mediated lysine capture by UBE2E1 reveals substrate-targeting mode of a ubiquitin-conjugating E2.
Brain Injuries
A Combined Proteomics and Bioinformatics Approach Reveals Novel Signaling Pathways and Molecular Targets After Intracerebral Hemorrhage.
Brain Ischemia
Circular RNA circ_HECTD1 regulates cell injury after cerebral infarction by miR-27a-3p/FSTL1 axis.
Breast Neoplasms
Aberrant recombination and repair during immunoglobulin class switching in BRCA1-deficient human B cells.
Breast Neoplasms
Drug mechanism-of-action discovery through the integration of pharmacological and CRISPR screens.
Breast Neoplasms
KSP inhibitor SB743921 inhibits growth and induces apoptosis of breast cancer cells by regulating p53, Bcl-2, and DTL.
Carcinogenesis
Constitutional Translocation Breakpoint Mapping by Genome-wide Paired-end Sequencing Identifies HACE1 as a Putative Wilms Tumor Susceptibility Gene.
Carcinogenesis
HACE1 reduces oxidative stress and mutant Huntingtin toxicity by promoting the NRF2 response.
Carcinogenesis
NEDD4 promotes cell growth and migration via PTEN/PI3K/AKT signaling in hepatocellular carcinoma.
Carcinoma, Lobular
Aberrations of Chromosomes 1 and 16 in Breast Cancer: A Framework for Cooperation of Transcriptionally Dysregulated Genes.
Cholangiocarcinoma
XIAP Antagonist Embelin Inhibited Proliferation of Cholangiocarcinoma Cells.
Colitis, Ulcerative
RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis.
Dyskinesias
L-DOPA impairs proteasome activity in parkinsonism through D1 dopamine receptor.
Esophageal Achalasia
Autophagy-related 16-like 1 is influenced by human herpes virus 1-encoded microRNAs in biopsy samples from the lower esophageal sphincter muscle during per-oral endoscopic myotomy for esophageal achalasia.
Fanconi Anemia
Aberrant recombination and repair during immunoglobulin class switching in BRCA1-deficient human B cells.
Glioma
Ectopic expression of E3 ubiquitin-protein ligase 2 in glioma and enhances resistance to apoptosis through activating nuclear factor ?-light-chain-enhancer of B cells.
Herpes Simplex
Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription.
Infections
Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription.
Infections
HACE1, an E3 ubiquitin-protein ligase, Mitigates Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Infection Induced Oxidative Stress by Promoting Nrf2 Activity.
Insulin Resistance
Cbl Proto-Oncogene B (CBLB) c.197A>T Mutation Induces Mild Metabolic Dysfunction in Partial Type I Multiple Symmetric Lipomatosis (MSL).
Intellectual Disability
Erratum to: Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.
Intellectual Disability
Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.
Language Development Disorders
Erratum to: Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.
Language Development Disorders
Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.
Liver Cirrhosis
[Effects of bone morphogenetic protein-7 therapy on E3 ubiquitin ligase expression in mouse liver with experimentally induced fibrosis].
Liver Diseases
Overexpression of ring finger protein 20 inhibits the progression of liver fibrosis via mediation of histone H2B lysine 120 ubiquitination.
Lung Neoplasms
Effects of RING-SH2Grb², a Chimeric Protein Containing the E3 Ligase Domain of Cbl, on the EGFR Pathway.
Lymphoma
Effects of RING-SH2Grb², a Chimeric Protein Containing the E3 Ligase Domain of Cbl, on the EGFR Pathway.
Lymphoma
The anergy induction of M3 muscarinic acetylcholine receptor-reactive CD4+ T cells suppresses experimental sialadenitis-like Sjögren's syndrome.
Motor Neuron Disease
Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
Mouth Neoplasms
Molecular docking data of E3 ubiquitin-protein ligase WWP1 with compounds from a medicinal plant Justicia adhatoda L.
Multiple Myeloma
Targeting of the E3 ubiquitin-protein ligase HUWE1 impairs DNA repair capacity and tumor growth in preclinical multiple myeloma models.
Muscular Atrophy
Small Molecular Weight Soybean Protein-Derived Peptides Nutriment Attenuates Rat Burn Injury-Induced Muscle Atrophy by Modulation of Ubiquitin-Proteasome System and Autophagy Signaling Pathway.
Neoplasm Metastasis
Integrative proteomics reveals the role of E3 ubiquitin ligase SYVN1 in hepatocellular carcinoma metastasis.
Neoplasm Metastasis
Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis.
Neoplasms
A HECT E3 ubiquitin-protein ligase with sequence similarity to E6AP does not target p53 for degradation in the softshell clam (Mya arenaria).
Neoplasms
Degradation of the proto-oncogene product c-Fos by the ubiquitin proteolytic system in vivo and in vitro: identification and characterization of the conjugating enzymes.
Neoplasms
HACE1 reduces oxidative stress and mutant Huntingtin toxicity by promoting the NRF2 response.
Neoplasms
HIF-1alpha and EPAS ubiquitination mediated by the VHL tumour suppressor involves flexibility in the ubiquitination mechanism, similar to other RING E3 ligases.
Neoplasms
Identification of CDC20 as an immune infiltration-correlated prognostic biomarker in hepatocellular carcinoma.
Neoplasms
Integrative proteomics reveals the role of E3 ubiquitin ligase SYVN1 in hepatocellular carcinoma metastasis.
Neoplasms
Interruption of MDM2 signaling augments MDM2-targeted T cell-based antitumor immunotherapy through antigen-presenting machinery.
Neoplasms
Loss of H2B monoubiquitination is associated with poor-differentiation and enhanced malignancy of lung adenocarcinoma.
Neoplasms
MiR-30 Family Potentially Targeting PI3K-SIAH2 Predicted Interaction Network Represents a Novel Putative Theranostic Panel in Non-small Cell Lung Cancer.
Neoplasms
Monoubiquitination is critical for ovarian tumor domain-containing ubiquitin aldehyde binding protein 1 (Otub1) to suppress UbcH5 enzyme and stabilize p53 protein.
Neoplasms
NEDD4 promotes cell growth and migration via PTEN/PI3K/AKT signaling in hepatocellular carcinoma.
Neoplasms
Pathogen-associated molecular patterns activate expression of genes involved in cell proliferation, immunity and detoxification in the amebocyte-producing organ of the snail Biomphalaria glabrata.
Neoplasms
Stability of the HTLV-1 Antisense-Derived Protein, HBZ, Is Regulated by the E3 Ubiquitin-Protein Ligase, UBR5.
Neoplasms
Targeting of the E3 ubiquitin-protein ligase HUWE1 impairs DNA repair capacity and tumor growth in preclinical multiple myeloma models.
Neoplasms
The E2 ubiquitin-conjugating enzyme UbcH5c: an emerging target in cancer and immune disorders.
Neoplasms
The effects of curcumin on proliferation, apoptosis, invasion, and NEDD4 expression in pancreatic cancer.
Neoplasms
The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation.
Neoplasms
The von Hippel-Lindau tumor suppressor protein is a component of an E3 ubiquitin-protein ligase activity.
Neuroinflammatory Diseases
MiR-142a-3p and miR-155-5p reduce methamphetamine-induced inflammation: Role of the target protein Peli1.
Neuroinflammatory Diseases
RNF216 mediates neuronal injury following experimental subarachnoid hemorrhage through the Arc/Arg3.1-AMPAR pathway.
Obesity
Cbl Proto-Oncogene B (CBLB) c.197A>T Mutation Induces Mild Metabolic Dysfunction in Partial Type I Multiple Symmetric Lipomatosis (MSL).
Parkinson Disease
Biochemical analysis of Parkinson's disease-causing variants of Parkin, an E3 ubiquitin-protein ligase with monoubiquitylation capacity.
Parkinson Disease
Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
Parkinson Disease
Parkin depletion delays motor decline dose-dependently without overtly affecting neuropathology in ?-synuclein transgenic mice.
Parkinson Disease
Parkin maintains mitochondrial levels of the protective Parkinson's disease-related enzyme 17-? hydroxysteroid dehydrogenase type 10.
Parkinson Disease
Parkin Protects Against Misfolded SOD1 Toxicity by Promoting Its Aggresome Formation and Autophagic Clearance.
Parkinsonian Disorders
Biochemical analysis of Parkinson's disease-causing variants of Parkin, an E3 ubiquitin-protein ligase with monoubiquitylation capacity.
Parkinsonian Disorders
Parkin-deficient mice are not more sensitive to 6-hydroxydopamine or methamphetamine neurotoxicity.
Parkinsonian Disorders
The p38 subunit of the aminoacyl-tRNA synthetase complex is a Parkin substrate: linking protein biosynthesis and neurodegeneration.
Parkinsonian Disorders
Ultrastructural localization of parkin in the rat brainstem, thalamus and basal ganglia.
Peste-des-Petits-Ruminants
Quantitative Proteomics Reveals a Novel Role of the E3 Ubiquitin-Protein Ligase FANCL in the Activation of the Innate Immune Response through Regulation of TBK1 Phosphorylation during Peste des Petits Ruminants Virus Infection.
Retinoblastoma
Function of cell-cycle regulators in predicting silent pituitary adenoma progression following surgical resection.
Spinocerebellar Ataxias
Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
Tauopathies
S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.
Virus Diseases
Quantitative Proteomics Reveals a Novel Role of the E3 Ubiquitin-Protein Ligase FANCL in the Activation of the Innate Immune Response through Regulation of TBK1 Phosphorylation during Peste des Petits Ruminants Virus Infection.
Wilms Tumor
Differential expression of a novel ankyrin containing E3 ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus normal kidney.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
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depletion of enzyme-dependent neddylation of the DNA-dependent protein kinase catalytic subunit reduces the efficiency of non-homologous end-joining
malfunction
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peptide-driven blocking of E2-to-HECT ubiquitin loading may limit SARS-CoV-2 egression and spread in the host cells
physiological function
part of the E1-E2-E3 ubiquitin conjugation cascade. The E2-ubiquitin covalent complex interacts with an E3 (HECT) for ubiquitin transfer. Ubiquitin is transferred to the acceptor cysteine of the C-lobe of the HECT domain
physiological function
itch ligase activities are controlled via autoinhibition. Aberrant activation of Itch leads to migration defects of cortical neurons during development
physiological function
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the enzyme is responsible for the neddylation of the DNA-dependent protein kinase catalytic subunit, the core component of DNA-PK complex in the non-homologous end-joining repair of DNA double-strand breaks
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). UB2E1_MOUSE
193
0
21333
Swiss-Prot
Secretory Pathway (Reliability: 2)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
determination of the crystal of a complex between the HECT domain NEDD4L and the E2 UbcH5B bearing a covantly-linked ubiquitin at its active site to gain insights of the ubiquitin transfer from an associated E2 to the acceptor cysteine in the HECT domain C-lobe. HECT contains is bilobed, consists of N-terminal N-lobe and C-terminal C-lobe. The C-lobe is essential for the E2-to-E3 ubiquitin transfer and the N-lobe is essential for the the further protein substrate processivity, the transfer of the ubiquitin from E3 to the substrate
the WW domains proceeding the catalytic HECT domain play an inhibitory role by binding directly to HECT. The WW2 domain and a following linker allosterically lock HECT in an inactive state inhibiting E2-E3 transthiolation. Binding of the Ndfip1 adaptor or JNK1-mediated phosphorylation relieves the auto-inhibition of Itch in a WW2-dependent manner
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
generation of chimeric E2 enzymes consisting of different parts of human UbcH5 and murine UbcM3. Chimera consist of the N-terminal 62 amino acids of UbcH5 fused to the C-terminal 85 amino acids of UbcM3 and of the N-terminal 108 amino acids of UbcM3 fused to the C-terminal 85 amino acids of UbcH5, respectively. The ability to interact with E3 enzyme E6-AP maps to the C-terminal 85 amino acids of UbcH5 and particularly to a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2 enzymes
additional information
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generation of chimeric E2 enzymes consisting of different parts of human UbcH5 and murine UbcM3. Chimera consist of the N-terminal 62 amino acids of UbcH5 fused to the C-terminal 85 amino acids of UbcM3 and of the N-terminal 108 amino acids of UbcM3 fused to the C-terminal 85 amino acids of UbcH5, respectively. The ability to interact with E3 enzyme E6-AP maps to the C-terminal 85 amino acids of UbcH5 and particularly to a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2 enzymes
additional information
generation of chimeric E2 enzymes consisting of different parts of human UbcH5 and murine UbcM3. Chimera consist of the N-terminal 62 amino acids of UbcH5 fused to the C-terminal 85 amino acids of UbcM3 and of the N-terminal 108 amino acids of UbcM3 fused to the C-terminal 85 amino acids of UbcH5, respectively. The ability to interact with E3 enzyme E6-AP maps to the C-terminal 85 amino acids of UbcH5
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography or glutathione Sepharose column chromatography and gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nuber, U.; Scheffner, M.
Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction
J. Biol. Chem.
274
7576-7582
1999
Homo sapiens (P51668), Homo sapiens, Mus musculus (P52482)
brenda
Kamadurai, H.B.; Souphron, J.; Scott, D.C.; Duda, D.M.; Miller, D.J.; Stringer, D.; Piper, R.C.; Schulman, B.A.
Insights into ubiquitin transfer cascades from a structure of a UbcH5B approximately ubiquitin-HECT(NEDD4L) complex
Mol. Cell.
36
1095-1102
2009
Homo sapiens (Q96PU5), Homo sapiens (P62837)
brenda
Riling, C.; Kamadurai, H.; Kumar, S.; OLeary, C.E.; Wu, K.P.; Manion, E.E.; Ying, M.; Schulman, B.A.; Oliver, P.M.
Itch WW domains inhibit its E3 ubiquitin ligase activity by blocking E2-E3 ligase trans-thiolation
J. Biol. Chem.
290
23875-23887
2015
Mus musculus (Q8C863)
brenda
Schumacher, F.R.; Wilson, G.; Day, C.L.
The N-terminal extension of UBE2E ubiquitin-conjugating enzymes limits chain assembly
J. Mol. Biol.
425
4099-4111
2013
Homo sapiens
brenda
Banka, P.A.; Behera, A.P.; Sarkar, S.; Datta, A.B.
RING E3-catalyzed E2 self-ubiquitination attenuates the activity of Ube2E ubiquitin-conjugating enzymes
J. Mol. Biol.
427
2290-2304
2015
Homo sapiens
brenda
Guo, Z.; Wang, S.; Xie, Y.; Han, Y.; Hu, S.; Guan, H.; Xie, D.; Bai, C.; Liu, X.; Gu, Y.; Zhou, P.K.; Ma, T.
HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response
Cell Death Dis.
11
400
2020
Homo sapiens
brenda
Zhu, K.; Shan, Z.; Chen, X.; Cai, Y.; Cui, L.; Yao, W.; Wang, Z.; Shi, P.; Tian, C.; Lou, J.; Xie, Y.; Wen, W.
Allosteric auto-inhibition and activation of the Nedd4 family E3 ligase Itch
EMBO Rep.
18
1618-1630
2017
Homo sapiens (Q96J02), Mus musculus (Q8C863)
brenda
Zahid, S.; Gul, M.; Shafique, S.; Rashid, S.
E2UbcH5B-derived peptide ligands target HECT E3-E2 binding site and block the Ub-dependent SARS-CoV-2 egression A computational study
Comput. Biol. Med.
146
105660
2022
Homo sapiens
brenda
Magnati, S.; Alladio, E.; Bracco, E.
A Survey on the expression of the ubiquitin proteasome system components HECT- and RBR-E3 ubiquitin ligases and E2 ubiquitin-conjugating and E1 ubiquitin-activating enzymes during human brain development
Int. J. Mol. Sci.
25
2361
2024
Homo sapiens
brenda
Mishra, V.; Crespo-Puig, A.; McCarthy, C.; Masonou, T.; Glegola-Madejska, I.; Dejoux, A.; Dow, G.; Eldridge, M.J.G.; Marinelli, L.H.; Meng, M.; Wang, S.; Bennison, D.J.; Morrison, R.; Shenoy, A.R.
IL-1beta turnover by the UBE2L3 ubiquitin conjugating enzyme and HECT E3 ligases limits inflammation
Nat. Commun.
14
4385
2023
Mus musculus, Homo sapiens
brenda
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