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Information on EC 2.3.2.B2 - alanyl-lipid II:alanine alanyl transferase
for references in articles please use BRENDA:EC2.3.2.B2
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preliminary BRENDA-supplied EC number
EC Tree 2 Transferases
2.3 Acyltransferases
2.3.2 Aminoacyltransferases
2.3.2.B2 alanyl-lipid II:alanine alanyl transferase
The expected taxonomic range for this enzyme is: Streptococcus pneumoniae
Reaction Schemes
showSynonyms
lipid II-Ala:alanyl-tRNA ligase, MurN, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
lipid II-Ala:alanyl-tRNA ligase
MurN
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA = UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
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-
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SYSTEMATIC NAME
IUBMB Comments
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine:alanine N6-alanyltransferase
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
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Substrates: i.e. N6-alanyl-lipid I
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: -
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
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Substrates: i.e. N6-alanyl-lipid I
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
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Substrates: -
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
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Substrates: i.e. N6-alanyl-lipid I
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: -
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
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Substrates: i.e. N6-seryl-lipid I
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: -
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: i.e. N6-seryl-lipid I
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: -
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: i.e. N6-seryl-lipid I
Products: -
Products: -
?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
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Substrates: no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I
Products: -
Products: -
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
33.1
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
40
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
125
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
146
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.02
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
0.06
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
0.07
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.579 - 2.186
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
1.579
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
2.186
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
0.112
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
0.138
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine
-
pH 6.8, 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.7
-
substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine, pH 6.8, 37°C
0.9
-
substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine, pH 6.8, 37°C
11
-
substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine, pH 6.8, 37°C
8.7
-
substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine, pH 6.8, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
enzyme is involved in formation of branched peptidoglycan peptide cross-bridges L-Ala-L-Ala or L-Ser-L-Ala in Streptococcus pneumoniae
physiological function
selective inactivation of the murN gene causes production of an unusual peptidoglycan that contains only single amino acid residues in the muropeptide branches
physiological function
-
selective inactivation of the murN gene causes production of an unusual peptidoglycan that contains only single amino acid residues in the muropeptide branches
-
physiological function
-
enzyme is involved in formation of branched peptidoglycan peptide cross-bridges L-Ala-L-Ala or L-Ser-L-Ala in Streptococcus pneumoniae
-
physiological function
-
enzyme is involved in formation of branched peptidoglycan peptide cross-bridges L-Ala-L-Ala or L-Ser-L-Ala in Streptococcus pneumoniae
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Filipe, s.R.; Pinho, M.G.; Tomasz, A.
Characterization of the murMN operon involved in the synthesis of branched peptidoglycan peptides in Streptococcus pneumoniae
J. Biol. Chem.
275
27768-27774
2000
Streptococcus pneumoniae (Q9L445), Streptococcus pneumoniae Pen6 (Q9L445)
brenda
De Pascale, G.; Lloyd, A.J.; Schouten, J.A.; Gilbey, a.M.; Roper, D.I.; Dowson, C.G.; Bugg, T.D.H.
Kinetic characterization of lipid II-Ala:alanyl-tRNA ligase (MurN) from Streptococcus pneumoniae using semisynthetic aminoacyl-lipid II substrates
J. Biol. Chem.
283
34751-34759
2008
Streptococcus pneumoniae, Streptococcus pneumoniae 159, Streptococcus pneumoniae Pn16
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brenda
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