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Information on EC 2.3.2.B18 - ergosteryl-3beta-O-glycine synthase
for references in articles please use BRENDA:EC2.3.2.B18
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Please check here for current data: EC unknown
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preliminary BRENDA-supplied EC number
EC Tree 2 Transferases
2.3 Acyltransferases
2.3.2 Aminoacyltransferases
2.3.2.B18 ergosteryl-3beta-O-glycine synthase
The enzyme appears in viruses and cellular organisms
showSynonyms
AFUA_8g01260, AO090011000521, DUF2156 domain-containing protein, ergosteryl-3beta-O-glycine synthase, ErgS, YALI0E00330g, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AFUA_8g01260
AO090011000521
DUF2156 domain-containing protein
ergosteryl-3beta-O-glycine synthase
ErgS
YALI0E00330g
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ergosterol + glycyl-tRNAGly = ergosteryl-3beta-O-glycine + tRNAGly
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-
-
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SYSTEMATIC NAME
IUBMB Comments
gylcyl:ergosterol glycyltransferase [tRNAGly specific]
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
additional information
?
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Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
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additional information
?
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Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
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additional information
?
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Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
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additional information
?
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Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
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additional information
?
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Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
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additional information
?
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Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + ergosterol
AMP + diphosphate + ergosteryl-3beta-O-glycine
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ATP + glycine + tRNAGly
AMP + diphosphate + L-glycyl-tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
ergosterol + L-glycyl-tRNAGly
ergosteryl-3beta-O-glycine + tRNAGly
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: glycyl-adenylate
Products: -
Products: -
?
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
evolution
ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota
evolution
ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota
evolution
ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota
evolution
-
ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota
-
evolution
-
ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota
-
evolution
-
ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q2U0A3_ASPOR
Aspergillus oryzae (strain ATCC 42149 / RIB 40)
379
0
41499
TrEMBL
-
Q4WB70_ASPFU
Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
549
0
62039
TrEMBL
-
Q6C7J3_YARLI
Yarrowia lipolytica (strain CLIB 122 / E 150)
471
0
52893
TrEMBL
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as Maltose-binding protein fusionand with HA-tag
expression in Escherichia coli as Maltose-binding protein fusions
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yakobov, N.; Mahmoudi, N.; Grob, G.; Yokokawa, D.; Saga, Y.; Kushiro, T.; Worrell, D.; Roy, H.; Schaller, H.; Senger, B.; Huck, L.; Riera Gascon, G.; Becker, H.D.; Fischer, F.
RNA-dependent synthesis of ergosteryl-3beta-O-glycine in Ascomycota expands the diversity of steryl-amino acids
J. Biol. Chem.
2022
101657
2022
Aspergillus fumigatus (Q4WB70), Aspergillus fumigatus ATCC MYA-4609 (Q4WB70), Aspergillus oryzae (Q2U0A3), Aspergillus oryzae ATCC 42149 (Q2U0A3), Yarrowia lipolytica (Q6C7J3), Yarrowia lipolytica CLIB 122 (Q6C7J3)
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EXTERNAL LINKS (specific for EC-Number 2.3.2.B18)
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Release 2026.1 (March 2026)
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