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Information on EC 2.3.2.34 - E2 NEDD8-conjugating enzyme

for references in articles please use BRENDA:EC2.3.2.34

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IUBMB Comments

Some RING-type E3 ubiquitin transferases (EC 2.3.2.27) are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. EC 6.2.1.64, E1 NEDD8-activating enzyme, activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of an E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC 2.3.2.32, cullin-RING-type E3 NEDD8 transferase).

The enzyme appears in viruses and cellular organisms
  • 2.3.2.34
  • cullins
  • ligases
  • ubiquitin-like
  • cullin-ring
  • nedd8-activating
  • rbx1
  • cullin-based
  • pevonedistat
Reaction Schemes
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
+
[E2 NEDD8-conjugating enzyme]-L-cysteine
=
[E1 NEDD8-activating enzyme]-L-cysteine
+
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine

Synonyms
ubc12, ube2m, ube2f, nedd8-conjugating enzyme, nedd8 e2, e2 nedd8-conjugating enzyme, nedd8-conjugating enzyme e2, neddylation e2-conjugating enzyme, e2 neddylation conjugating enzyme, more

REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
show the reaction diagram
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-
-
-
PATHWAY SOURCE
PATHWAYS
MetaCyc
protein NEDDylation
Highest Expressing Human Cell Lines
Cell Line Links Gene Links