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IUBMB Comments The enzyme participates in the N-end rule protein degradation pathway in certain bacteria, by attaching the primary destabilizing residue L -leucine to the N-termini of proteins that have an N-terminal L -aspartate or L -glutamate residue. Once modified, the proteins are recognized by EC 3.4.21.92 , the ClpAP/ClpS endopeptidase system. cf . EC 2.3.2.6 , lysine/arginine leucyltransferase, and EC 2.3.2.8 , arginyltransferase.
Reaction Schemes
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N-terminal L-glutamyl-[protein]
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N-terminal L-leucyl-L-glutamyl-[protein]
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N-terminal L-aspartyl-[protein]
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N-terminal L-leucyl-L-aspartyl-[protein]
Synonyms BPT , LD,E-transferase, Leu-conjugating aa-transferase, leucylD,E-transferase,
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bacterial protein transferase
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Leu-conjugating aa-transferase
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leucylD,E-transferase
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BPT
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L-leucyl-tRNALeu + N-terminal L-aspartyl-[protein] = tRNALeu + N-terminal L-leucyl-L-aspartyl-[protein]
(2)
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L-leucyl-tRNALeu + N-terminal L-glutamyl-[protein] = tRNALeu + N-terminal L-leucyl-L-glutamyl-[protein]
(1)
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MetaCyc
N-end rule pathway II (prokaryotic)
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L-leucyl-tRNALeu:[protein] N-terminal L-glutamate/L-aspartate leucyltransferase
The enzyme participates in the N-end rule protein degradation pathway in certain bacteria, by attaching the primary destabilizing residue L-leucine to the N-termini of proteins that have an N-terminal L-aspartate or L-glutamate residue. Once modified, the proteins are recognized by EC 3.4.21.92, the ClpAP/ClpS endopeptidase system. cf. EC 2.3.2.6, lysine/arginine leucyltransferase, and EC 2.3.2.8, arginyltransferase.
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L-leucyl-tRNALeu + N-terminal L-aspartyl-[protein]
tRNALeu + N-terminal L-leucyl-L-aspartyl-[protein]
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Substrates: -
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L-leucyl-tRNALeu + N-terminal L-glutamyl-[protein]
tRNALeu + N-terminal L-leucyl-L-glutamyl-[protein]
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Substrates: -
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L-leucyl-tRNALeu + N-terminal L-aspartyl-[protein]
tRNALeu + N-terminal L-leucyl-L-aspartyl-[protein]
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Substrates: -
?
L-leucyl-tRNALeu + N-terminal L-glutamyl-[protein]
tRNALeu + N-terminal L-leucyl-L-glutamyl-[protein]
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Substrates: -
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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Y170F
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the mutant loses 90% of wild type enzyme activity
Y205F
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the mutant loses 90% of wild type enzyme activity
Y58F
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the mutant loses 70-80% of wild type enzyme activity
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expressed in Escherichia coli KPS22 cells
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Graciet, E.; Hu, R.; Piatkov, K.; Rhee, J.; Schwarz, E.; Varshavsky, A.
Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen
Proc. Natl. Acad. Sci. USA
103
3078-3083
2006
Vibrio vulnificus
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