Information on EC 2.3.2.11 - alanylphosphatidylglycerol synthase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.3.2.11
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RECOMMENDED NAME
GeneOntology No.
alanylphosphatidylglycerol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminoacyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
L-alanyl-tRNAAla:phosphatidylglycerol alanyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37257-27-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Clostridium welchii
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Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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MprF1-mediated alanyl-phosphatidylglycerol production in Staphylococcus aureus does not affect susceptibility to gallidermin and nisin and leads to only a minor decrease in daptomycin susceptibility. Coexpression of the enzyme with flippase domains of lysyl-phosphatidylglycerol synthesizing MprF proteins leads to a wild type level of daptomycin susceptibility
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
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20% activity compared to phosphatidylglycerol
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?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
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20% activity compared to phosphatidylglycerol
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-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
microhelix A + phosphatidylglycerol
?
show the reaction diagram
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90% activity compared to L-alanyl-tRNA
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?
microhelix B + phosphatidylglycerol
?
show the reaction diagram
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40% activity compared to L-alanyl-tRNA
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-
?
microhelix C + phosphatidylglycerol
?
show the reaction diagram
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?
microhelix D + phosphatidylglycerol
?
show the reaction diagram
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-
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-
?
microhelix E + phosphatidylglycerol
?
show the reaction diagram
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90% activity compared to L-alanyl-tRNA
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
additional information
?
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although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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enzyme catalysis is not dependent on metal ions
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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20% residual activity at 20 mM
additional information
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in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
Clostridium welchii
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7.8
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in vivo activity assay
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Clostridium welchii
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assay at
35
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in vitro activity assay; in vivo activity assay
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
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gel filtration
38000
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1 * 38000, SDS-PAGE
38720
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1 * 38720, calculated from amino acid sequence
84000
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determined by SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 38000, SDS-PAGE; 1 * 38720, calculated from amino acid sequence
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
catalytic domain, hanging drop vapor diffusion method, using 7.5 mM CoCl2, 85 mM Mes (pH 5.7), 1.53 M (NH4)2SO4, and 15% (v/v) glycerol or 85 mM Na-acetate (pH 6.37), 1.6 M (NH4)2SO4, and 20% (v/v) glycerol
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes during storage
Clostridium welchii
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 weeks, 10 mM Tris/HCl, pH 8.0, 10 mM 2-mercaptoethanol, 30% glycerol
Clostridium welchii
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-60°C, several months
Clostridium welchii
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions
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glutathione Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells
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expressed in a mprF deletion mutant of Staphylococcus aureus
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expressed in Escherichia coli BL21(DE3) cells
into the vectors pET22b+, pUCP20T, pBAD-His-A, pBADmyc-His-A
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression is found significantly up-regulated under acidic conditions
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ENGINEERING
ORGANISM
UNIPROT
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LITERATURE
D579A
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the mutant shows decreased activity (3%) compared to the wild type enzyme
D579N
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the mutant shows decreased activity (5%) compared to the wild type enzyme
D710A
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the mutant has 26% of wild type activity
D710N
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the mutant has 30% of wild type activity
D765A
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inactive
D765N
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inactive
E657D
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the mutant shows decreased activity (4%) compared to the wild type enzyme
E657Q
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the mutant has 21% of wild type activity
E658D
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the mutant has 28% of wild type activity
E658Q
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the mutant has 19% of wild type activity
E720Q
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the mutant has 10% of wild type activity
K580Q
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inactive
K580S
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inactive
K654Q
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the mutant has 65% of wild type activity
K654S
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the mutant has 46% of wild type activity
K840Q
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inactive
K840S
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inactive
K842Q
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the mutant has wild type activity
K842S
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the mutant has 50% of wild type activity
M778A
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the mutant has 50% of wild type activity
R768Q
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inactive
R768S
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inactive
R837Q
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the mutant has wild type activity
R837S
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the mutant has 65% of wild type activity
S709A
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the mutant has 5% of wild type activity
S709N
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the mutant has 17% of wild type activity
S724A
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the mutant shows decreased activity (7%) compared to the wild type enzyme
S724N
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inactive
S763A
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the mutant has 23% of wild type activity
S763N
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the mutant has 11% of wild type activity
Y635A
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inactive
Y732A
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the mutant shows decreased activity (3%) compared to the wild type enzyme
Y831A
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inactive
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.2.11)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)