We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
a-pgs, alanyl-phosphatidylglycerol synthase, a-pg synthase, mprf1, ala-pg synthase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
alanyl phosphatidylglycerol synthetase
-
-
-
alanyl-phosphatidylglycerol synthase
alanylphosphatidylglycerol synthase
-
-
O-alanylphosphatidylglycerol synthase
-
-
-
synthase, O-alanylphosphatidylglycerol
-
-
-
A-PGS
-
-
Ala-PG synthase
-
-
alanyl-phosphatidylglycerol synthase
-
-
alanyl-phosphatidylglycerol synthase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
aminoacyl group transfer
-
-
-
aminoacyl group transfer
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNAAla:phosphatidylglycerol alanyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
20% activity compared to phosphatidylglycerol
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
20% activity compared to phosphatidylglycerol
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
microhelix A + phosphatidylglycerol
?
-
90% activity compared to L-alanyl-tRNA
-
?
microhelix B + phosphatidylglycerol
?
-
40% activity compared to L-alanyl-tRNA
-
?
microhelix C + phosphatidylglycerol
?
-
-
-
?
microhelix D + phosphatidylglycerol
?
-
-
-
?
microhelix E + phosphatidylglycerol
?
-
90% activity compared to L-alanyl-tRNA
-
?
additional information
?
-
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
100% activity compared to phosphatidylglycerol
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
Clostridium welchii
-
other L-alanyl-tRNAs than L-alanyl-tRNAAla are no substrates
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
100% activity
-
?
additional information
?
-
Clostridium welchii
-
no substrates are N-acetylalanyl-tRNA, lactyl-tRNA, alanyl-tRNACys and phenylalanyl-tRNAAla
-
?
additional information
?
-
-
although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
-
?
additional information
?
-
-
1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
additional information
?
-
-
although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
enzyme catalysis is not dependent on metal ions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1,10-phenanthroline
-
20% residual activity at 20 mM
additional information
-
in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.7
Clostridium welchii
-
-
7.8
-
in vivo activity assay
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30
Clostridium welchii
-
assay at
35
-
in vitro activity assay
35
-
in vivo activity assay
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Clostridium welchii
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
Clostridium welchii
-
-
brenda
-
a transmembrane protein which is located in the inner membrane
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
MprF1-mediated alanyl-phosphatidylglycerol production in Staphylococcus aureus does not affect susceptibility to gallidermin and nisin and leads to only a minor decrease in daptomycin susceptibility. Coexpression of the enzyme with flippase domains of lysyl-phosphatidylglycerol synthesizing MprF proteins leads to a wild type level of daptomycin susceptibility
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Q9I537_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
881
14
95862
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
38000
-
1 * 38000, SDS-PAGE
38720
-
1 * 38720, calculated from amino acid sequence
84000
-
determined by SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
monomer
-
1 * 38000, SDS-PAGE
monomer
-
1 * 38720, calculated from amino acid sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
catalytic domain, hanging drop vapor diffusion method, using 7.5 mM CoCl2, 85 mM Mes (pH 5.7), 1.53 M (NH4)2SO4, and 15% (v/v) glycerol or 85 mM Na-acetate (pH 6.37), 1.6 M (NH4)2SO4, and 20% (v/v) glycerol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D579A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
D579N
-
the mutant shows decreased activity (5%) compared to the wild type enzyme
D710A
-
the mutant has 26% of wild type activity
D710N
-
the mutant has 30% of wild type activity
E657D
-
the mutant shows decreased activity (4%) compared to the wild type enzyme
E657Q
-
the mutant has 21% of wild type activity
E658D
-
the mutant has 28% of wild type activity
E658Q
-
the mutant has 19% of wild type activity
E720Q
-
the mutant has 10% of wild type activity
K654Q
-
the mutant has 65% of wild type activity
K654S
-
the mutant has 46% of wild type activity
K842Q
-
the mutant has wild type activity
K842S
-
the mutant has 50% of wild type activity
M778A
-
the mutant has 50% of wild type activity
R837Q
-
the mutant has wild type activity
R837S
-
the mutant has 65% of wild type activity
S709A
-
the mutant has 5% of wild type activity
S709N
-
the mutant has 17% of wild type activity
S724A
-
the mutant shows decreased activity (7%) compared to the wild type enzyme
S763A
-
the mutant has 23% of wild type activity
S763N
-
the mutant has 11% of wild type activity
Y732A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
glycerol stabilizes during storage
Clostridium welchii
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 3 weeks, 10 mM Tris/HCl, pH 8.0, 10 mM 2-mercaptoethanol, 30% glycerol
Clostridium welchii
-
-60°C, several months
Clostridium welchii
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions
-
glutathione Sepharose column chromatography
glutathione Sepharose column chromatography
-
glutathione Sepharose column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells
-
expressed in a mprF deletion mutant of Staphylococcus aureus
-
expressed in Escherichia coli BL21(DE3) cells
into the vectors pET22b+, pUCP20T, pBAD-His-A, pBADmyc-His-A
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene expression is found significantly up-regulated under acidic conditions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Gould, R.M.; Thornton, M.P.; Liepkalns, V.; Lennarz, W.J.
Participation of aminoacyl transfer ribonucleic acid in aminoacyl phosphatidylglycerol synthesis. II. Specificity of alanyl phosphatidylglycerol synthetase
J. Biol. Chem.
243
3096-3104
1968
Clostridium welchii
brenda
Roy, H.; Dare, K.; Ibba, M.
Adaptation of the bacterial membrane to changing environments using aminoacylated phospholipids: MicroCommentary
Mol. Microbiol.
71
547-550
2009
Pseudomonas aeruginosa
brenda
Klein, S.; Lorenzo, C.; Hoffmann, S.; Walther, J.; Storbeck, S.; Piekarski, T.; Tindall, B.; Wray, V.; Nimtz, M.; Moser, J.
Adaptation of Pseudomonas aeruginosa to various conditions includes tRNA-dependent formation of alanyl-phosphatidylglycerol
Mol. Microbiol.
71
551-565
2009
Pseudomonas aeruginosa
brenda
Slavetinsky, C.J.; Peschel, A.; Ernst, C.M.
Alanyl-phosphatidylglycerol and lysyl-phosphatidylglycerol are translocated by the same MprF flippases and have similar capacities to protect against the antibiotic daptomycin in Staphylococcus aureus
Antimicrob. Agents Chemother.
56
3492-3497
2012
Clostridium perfringens
brenda
Arendt, W.; Hebecker, S.; Jaeger, S.; Nimtz, M.; Moser, J.
Resistance phenotypes mediated by aminoacyl-phosphatidylglycerol synthases
J. Bacteriol.
194
1401-1416
2012
Pseudomonas aeruginosa
brenda
Hebecker, S.; Arendt, W.; Heinemann, I.U.; Tiefenau, J.H.; Nimtz, M.; Rohde, M.; Soell, D.; Moser, J.
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa
Mol. Microbiol.
80
935-950
2011
Pseudomonas aeruginosa
brenda
Smith, A.; Harrison, J.; Grube, C.; Sheppe, A.; Sahara, N.; Ishii, R.; Nureki, O.; Roy, H.
tRNA-dependent alanylation of diacylglycerol and phosphatidylglycerol in Corynebacterium glutamicum
Mol. Microbiol.
98
681-693
2015
Corynebacterium glutamicum
brenda
Hebecker, S.; Krausze, J.; Hasenkampf, T.; Schneider, J.; Groenewold, M.; Reichelt, J.; Jahn, D.; Heinz, D.W.; Moser, J.
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Proc. Natl. Acad. Sci. USA
112
10691-10696
2015
Pseudomonas aeruginosa (Q9I537), Pseudomonas aeruginosa
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
2.3.2.11 alanylphosphatidylglycerol synthase
more
top
Information
