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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
A-PG synthase, A-PGS, Ala-PG synthase, alanyl phosphatidylglycerol synthetase, alanyl-phosphatidylglycerol synthase, alanylphosphatidylglycerol synthase, Cg1103 protein, MprF1, O-alanylphosphatidylglycerol synthase, synthase, O-alanylphosphatidylglycerol,
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alanyl phosphatidylglycerol synthetase
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alanyl-phosphatidylglycerol synthase
alanylphosphatidylglycerol synthase
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O-alanylphosphatidylglycerol synthase
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synthase, O-alanylphosphatidylglycerol
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A-PGS
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Ala-PG synthase
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alanyl-phosphatidylglycerol synthase
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alanyl-phosphatidylglycerol synthase
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L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
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aminoacyl group transfer
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aminoacyl group transfer
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L-alanyl-tRNAAla:phosphatidylglycerol alanyltransferase
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L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)
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20% activity compared to phosphatidylglycerol
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L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
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L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)
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20% activity compared to phosphatidylglycerol
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
microhelix A + phosphatidylglycerol
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90% activity compared to L-alanyl-tRNA
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microhelix B + phosphatidylglycerol
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40% activity compared to L-alanyl-tRNA
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microhelix C + phosphatidylglycerol
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microhelix D + phosphatidylglycerol
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microhelix E + phosphatidylglycerol
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90% activity compared to L-alanyl-tRNA
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additional information
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L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
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L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
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100% activity compared to phosphatidylglycerol
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
Clostridium welchii
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other L-alanyl-tRNAs than L-alanyl-tRNAAla are no substrates
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
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100% activity
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additional information
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Clostridium welchii
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no substrates are N-acetylalanyl-tRNA, lactyl-tRNA, alanyl-tRNACys and phenylalanyl-tRNAAla
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additional information
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although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
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additional information
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1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
additional information
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although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
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L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
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additional information
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enzyme catalysis is not dependent on metal ions
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1,10-phenanthroline
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20% residual activity at 20 mM
additional information
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in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed
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5.7
Clostridium welchii
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7.8
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in vivo activity assay
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30
Clostridium welchii
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assay at
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in vitro activity assay
35
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in vivo activity assay
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brenda
Clostridium welchii
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brenda
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brenda
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brenda
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UniProt
brenda
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brenda
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brenda
Clostridium welchii
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brenda
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a transmembrane protein which is located in the inner membrane
brenda
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physiological function
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MprF1-mediated alanyl-phosphatidylglycerol production in Staphylococcus aureus does not affect susceptibility to gallidermin and nisin and leads to only a minor decrease in daptomycin susceptibility. Coexpression of the enzyme with flippase domains of lysyl-phosphatidylglycerol synthesizing MprF proteins leads to a wild type level of daptomycin susceptibility
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Q9I537_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
881
14
95862
TrEMBL
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38000
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1 * 38000, SDS-PAGE
38720
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1 * 38720, calculated from amino acid sequence
84000
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determined by SDS-PAGE
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monomer
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1 * 38000, SDS-PAGE
monomer
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1 * 38720, calculated from amino acid sequence
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catalytic domain, hanging drop vapor diffusion method, using 7.5 mM CoCl2, 85 mM Mes (pH 5.7), 1.53 M (NH4)2SO4, and 15% (v/v) glycerol or 85 mM Na-acetate (pH 6.37), 1.6 M (NH4)2SO4, and 20% (v/v) glycerol
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D579A
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the mutant shows decreased activity (3%) compared to the wild type enzyme
D579N
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the mutant shows decreased activity (5%) compared to the wild type enzyme
D710A
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the mutant has 26% of wild type activity
D710N
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the mutant has 30% of wild type activity
E657D
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the mutant shows decreased activity (4%) compared to the wild type enzyme
E657Q
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the mutant has 21% of wild type activity
E658D
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the mutant has 28% of wild type activity
E658Q
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the mutant has 19% of wild type activity
E720Q
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the mutant has 10% of wild type activity
K654Q
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the mutant has 65% of wild type activity
K654S
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the mutant has 46% of wild type activity
K842Q
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the mutant has wild type activity
K842S
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the mutant has 50% of wild type activity
M778A
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the mutant has 50% of wild type activity
R837Q
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the mutant has wild type activity
R837S
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the mutant has 65% of wild type activity
S709A
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the mutant has 5% of wild type activity
S709N
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the mutant has 17% of wild type activity
S724A
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the mutant shows decreased activity (7%) compared to the wild type enzyme
S763A
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the mutant has 23% of wild type activity
S763N
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the mutant has 11% of wild type activity
Y732A
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the mutant shows decreased activity (3%) compared to the wild type enzyme
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glycerol stabilizes during storage
Clostridium welchii
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-20°C, 3 weeks, 10 mM Tris/HCl, pH 8.0, 10 mM 2-mercaptoethanol, 30% glycerol
Clostridium welchii
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-60°C, several months
Clostridium welchii
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from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions
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glutathione Sepharose column chromatography
glutathione Sepharose column chromatography
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glutathione Sepharose column chromatography
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enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells
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expressed in a mprF deletion mutant of Staphylococcus aureus
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expressed in Escherichia coli BL21(DE3) cells
into the vectors pET22b+, pUCP20T, pBAD-His-A, pBADmyc-His-A
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gene expression is found significantly up-regulated under acidic conditions
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Gould, R.M.; Thornton, M.P.; Liepkalns, V.; Lennarz, W.J.
Participation of aminoacyl transfer ribonucleic acid in aminoacyl phosphatidylglycerol synthesis. II. Specificity of alanyl phosphatidylglycerol synthetase
J. Biol. Chem.
243
3096-3104
1968
Clostridium welchii
brenda
Roy, H.; Dare, K.; Ibba, M.
Adaptation of the bacterial membrane to changing environments using aminoacylated phospholipids: MicroCommentary
Mol. Microbiol.
71
547-550
2009
Pseudomonas aeruginosa
brenda
Klein, S.; Lorenzo, C.; Hoffmann, S.; Walther, J.; Storbeck, S.; Piekarski, T.; Tindall, B.; Wray, V.; Nimtz, M.; Moser, J.
Adaptation of Pseudomonas aeruginosa to various conditions includes tRNA-dependent formation of alanyl-phosphatidylglycerol
Mol. Microbiol.
71
551-565
2009
Pseudomonas aeruginosa
brenda
Slavetinsky, C.J.; Peschel, A.; Ernst, C.M.
Alanyl-phosphatidylglycerol and lysyl-phosphatidylglycerol are translocated by the same MprF flippases and have similar capacities to protect against the antibiotic daptomycin in Staphylococcus aureus
Antimicrob. Agents Chemother.
56
3492-3497
2012
Clostridium perfringens
brenda
Arendt, W.; Hebecker, S.; Jaeger, S.; Nimtz, M.; Moser, J.
Resistance phenotypes mediated by aminoacyl-phosphatidylglycerol synthases
J. Bacteriol.
194
1401-1416
2012
Pseudomonas aeruginosa
brenda
Hebecker, S.; Arendt, W.; Heinemann, I.U.; Tiefenau, J.H.; Nimtz, M.; Rohde, M.; Soell, D.; Moser, J.
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa
Mol. Microbiol.
80
935-950
2011
Pseudomonas aeruginosa
brenda
Smith, A.; Harrison, J.; Grube, C.; Sheppe, A.; Sahara, N.; Ishii, R.; Nureki, O.; Roy, H.
tRNA-dependent alanylation of diacylglycerol and phosphatidylglycerol in Corynebacterium glutamicum
Mol. Microbiol.
98
681-693
2015
Corynebacterium glutamicum
brenda
Hebecker, S.; Krausze, J.; Hasenkampf, T.; Schneider, J.; Groenewold, M.; Reichelt, J.; Jahn, D.; Heinz, D.W.; Moser, J.
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Proc. Natl. Acad. Sci. USA
112
10691-10696
2015
Pseudomonas aeruginosa (Q9I537), Pseudomonas aeruginosa
brenda
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