BRENDA home
History
Information on EC 2.3.2.11 - alanylphosphatidylglycerol synthase
for references in articles please use BRENDA:EC2.3.2.11Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.3.2.11 alanylphosphatidylglycerol synthaseSpecify your search results
Word Map
- 2.3.2.11
- aminoacylation
- phospholipid
-
lysyl-phosphatidylglycerol
- aeruginosa
- synthases
-
trna-dependent
- aureus
- aminoacyl-trna
- flippase
- perfringens
- camps
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
A-PG synthase, A-PGS, Ala-PG synthase, alanyl phosphatidylglycerol synthetase, alanyl-phosphatidylglycerol synthase, alanylphosphatidylglycerol synthase, Cg1103 protein, MprF1, O-alanylphosphatidylglycerol synthase, synthase, O-alanylphosphatidylglycerol, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A-PGS
Ala-PG synthase
alanyl phosphatidylglycerol synthetase
-
-
-
-
alanyl-phosphatidylglycerol synthase
O-alanylphosphatidylglycerol synthase
-
-
-
-
synthase, O-alanylphosphatidylglycerol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
aminoacyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
L-alanyl-tRNAAla:phosphatidylglycerol alanyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37257-27-5
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
20% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
20% activity compared to phosphatidylglycerol
-
-
?
microhelix A + phosphatidylglycerol
?
-
90% activity compared to L-alanyl-tRNA
-
-
?
microhelix B + phosphatidylglycerol
?
-
40% activity compared to L-alanyl-tRNA
-
-
?
microhelix E + phosphatidylglycerol
?
-
90% activity compared to L-alanyl-tRNA
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
-
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
100% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
Clostridium welchii
-
other L-alanyl-tRNAs than L-alanyl-tRNAAla are no substrates
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
100% activity
-
-
?
additional information
?
-
Clostridium welchii
-
no substrates are N-acetylalanyl-tRNA, lactyl-tRNA, alanyl-tRNACys and phenylalanyl-tRNAAla
-
-
?
additional information
?
-
-
although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
-
-
?
additional information
?
-
-
1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
enzyme catalysis is not dependent on metal ions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
a transmembrane protein which is located in the inner membrane
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
MprF1-mediated alanyl-phosphatidylglycerol production in Staphylococcus aureus does not affect susceptibility to gallidermin and nisin and leads to only a minor decrease in daptomycin susceptibility. Coexpression of the enzyme with flippase domains of lysyl-phosphatidylglycerol synthesizing MprF proteins leads to a wild type level of daptomycin susceptibility
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q9I537_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
881
14
95862
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic domain, hanging drop vapor diffusion method, using 7.5 mM CoCl2, 85 mM Mes (pH 5.7), 1.53 M (NH4)2SO4, and 15% (v/v) glycerol or 85 mM Na-acetate (pH 6.37), 1.6 M (NH4)2SO4, and 20% (v/v) glycerol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D579A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
D579N
-
the mutant shows decreased activity (5%) compared to the wild type enzyme
E657D
-
the mutant shows decreased activity (4%) compared to the wild type enzyme
S724A
-
the mutant shows decreased activity (7%) compared to the wild type enzyme
Y732A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 weeks, 10 mM Tris/HCl, pH 8.0, 10 mM 2-mercaptoethanol, 30% glycerol
Clostridium welchii
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions
-
glutathione Sepharose column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression is found significantly up-regulated under acidic conditions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gould, R.M.; Thornton, M.P.; Liepkalns, V.; Lennarz, W.J.
Participation of aminoacyl transfer ribonucleic acid in aminoacyl phosphatidylglycerol synthesis. II. Specificity of alanyl phosphatidylglycerol synthetase
J. Biol. Chem.
243
3096-3104
1968
Clostridium welchii
brenda
Roy, H.; Dare, K.; Ibba, M.
Adaptation of the bacterial membrane to changing environments using aminoacylated phospholipids: MicroCommentary
Mol. Microbiol.
71
547-550
2009
Pseudomonas aeruginosa
brenda
Klein, S.; Lorenzo, C.; Hoffmann, S.; Walther, J.; Storbeck, S.; Piekarski, T.; Tindall, B.; Wray, V.; Nimtz, M.; Moser, J.
Adaptation of Pseudomonas aeruginosa to various conditions includes tRNA-dependent formation of alanyl-phosphatidylglycerol
Mol. Microbiol.
71
551-565
2009
Pseudomonas aeruginosa
brenda
Slavetinsky, C.J.; Peschel, A.; Ernst, C.M.
Alanyl-phosphatidylglycerol and lysyl-phosphatidylglycerol are translocated by the same MprF flippases and have similar capacities to protect against the antibiotic daptomycin in Staphylococcus aureus
Antimicrob. Agents Chemother.
56
3492-3497
2012
Clostridium perfringens
brenda
Arendt, W.; Hebecker, S.; Jaeger, S.; Nimtz, M.; Moser, J.
Resistance phenotypes mediated by aminoacyl-phosphatidylglycerol synthases
J. Bacteriol.
194
1401-1416
2012
Pseudomonas aeruginosa
brenda
Hebecker, S.; Arendt, W.; Heinemann, I.U.; Tiefenau, J.H.; Nimtz, M.; Rohde, M.; Soell, D.; Moser, J.
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa
Mol. Microbiol.
80
935-950
2011
Pseudomonas aeruginosa
brenda
Smith, A.; Harrison, J.; Grube, C.; Sheppe, A.; Sahara, N.; Ishii, R.; Nureki, O.; Roy, H.
tRNA-dependent alanylation of diacylglycerol and phosphatidylglycerol in Corynebacterium glutamicum
Mol. Microbiol.
98
681-693
2015
Corynebacterium glutamicum
brenda
Hebecker, S.; Krausze, J.; Hasenkampf, T.; Schneider, J.; Groenewold, M.; Reichelt, J.; Jahn, D.; Heinz, D.W.; Moser, J.
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Proc. Natl. Acad. Sci. USA
112
10691-10696
2015
Pseudomonas aeruginosa (Q9I537), Pseudomonas aeruginosa
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 2.3.2.11)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
About BRENDA
Help
Download
Contribution
member of
curated at
curated at
Server: brenda6 Ver: 69ad221-main (2022-06-24 14:26:09 +0200)