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Information on EC 2.3.2.11 - alanylphosphatidylglycerol synthase
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2.3.2.11 alanylphosphatidylglycerol synthaseSpecify your search results
Word Map
- 2.3.2.11
- aminoacylation
- phospholipid
-
lysyl-phosphatidylglycerol
- aeruginosa
- synthases
-
trna-dependent
- aureus
- aminoacyl-trna
-
opportunistic
- perfringens
- camps
- flippase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
A-PG synthase, A-PGS, Ala-PG synthase, alanyl phosphatidylglycerol synthetase, alanyl-phosphatidylglycerol synthase, alanylphosphatidylglycerol synthase, Cg1103 protein, MprF1, O-alanylphosphatidylglycerol synthase, synthase, O-alanylphosphatidylglycerol, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A-PGS
Ala-PG synthase
alanyl-phosphatidylglycerol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
L-alanyl-tRNAAla:phosphatidylglycerol alanyltransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
37257-27-5
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
20% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
20% activity compared to phosphatidylglycerol
-
-
?
microhelix A + phosphatidylglycerol
?
-
90% activity compared to L-alanyl-tRNA
-
-
?
microhelix B + phosphatidylglycerol
?
-
40% activity compared to L-alanyl-tRNA
-
-
?
microhelix E + phosphatidylglycerol
?
-
90% activity compared to L-alanyl-tRNA
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
-
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
-
100% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
Clostridium welchii
-
other L-alanyl-tRNAs than L-alanyl-tRNAAla are no substrates
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
100% activity
-
-
?
additional information
?
-
Clostridium welchii
-
no substrates are N-acetylalanyl-tRNA, lactyl-tRNA, alanyl-tRNACys and phenylalanyl-tRNAAla
-
-
?
additional information
?
-
-
although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
-
-
?
additional information
?
-
-
1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
although the enzyme protein Cg1103 is unable to synthesize 3-O-L-alanyl-1-O-phosphatidylglycerol in vitro or in vivo, 3-O-L-alanyl-1-O-phosphatidylglycerol synthesis can be reconstituted by coexpression of enzyme protein Cg1103 with PesT, a conserved putative esterase
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
enzyme catalysis is not dependent on metal ions
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
a transmembrane protein which is located in the inner membrane
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
MprF1-mediated alanyl-phosphatidylglycerol production in Staphylococcus aureus does not affect susceptibility to gallidermin and nisin and leads to only a minor decrease in daptomycin susceptibility. Coexpression of the enzyme with flippase domains of lysyl-phosphatidylglycerol synthesizing MprF proteins leads to a wild type level of daptomycin susceptibility
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q9I537_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
881
14
95862
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic domain, hanging drop vapor diffusion method, using 7.5 mM CoCl2, 85 mM Mes (pH 5.7), 1.53 M (NH4)2SO4, and 15% (v/v) glycerol or 85 mM Na-acetate (pH 6.37), 1.6 M (NH4)2SO4, and 20% (v/v) glycerol
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D579A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
D579N
-
the mutant shows decreased activity (5%) compared to the wild type enzyme
E657D
-
the mutant shows decreased activity (4%) compared to the wild type enzyme
S724A
-
the mutant shows decreased activity (7%) compared to the wild type enzyme
Y732A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 weeks, 10 mM Tris/HCl, pH 8.0, 10 mM 2-mercaptoethanol, 30% glycerol
Clostridium welchii
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions
-
glutathione Sepharose column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression is found significantly up-regulated under acidic conditions
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gould, R.M.; Thornton, M.P.; Liepkalns, V.; Lennarz, W.J.
Participation of aminoacyl transfer ribonucleic acid in aminoacyl phosphatidylglycerol synthesis. II. Specificity of alanyl phosphatidylglycerol synthetase
J. Biol. Chem.
243
3096-3104
1968
Clostridium welchii
brenda
Roy, H.; Dare, K.; Ibba, M.
Adaptation of the bacterial membrane to changing environments using aminoacylated phospholipids: MicroCommentary
Mol. Microbiol.
71
547-550
2009
Pseudomonas aeruginosa
brenda
Klein, S.; Lorenzo, C.; Hoffmann, S.; Walther, J.; Storbeck, S.; Piekarski, T.; Tindall, B.; Wray, V.; Nimtz, M.; Moser, J.
Adaptation of Pseudomonas aeruginosa to various conditions includes tRNA-dependent formation of alanyl-phosphatidylglycerol
Mol. Microbiol.
71
551-565
2009
Pseudomonas aeruginosa
brenda
Slavetinsky, C.J.; Peschel, A.; Ernst, C.M.
Alanyl-phosphatidylglycerol and lysyl-phosphatidylglycerol are translocated by the same MprF flippases and have similar capacities to protect against the antibiotic daptomycin in Staphylococcus aureus
Antimicrob. Agents Chemother.
56
3492-3497
2012
Clostridium perfringens
brenda
Arendt, W.; Hebecker, S.; Jaeger, S.; Nimtz, M.; Moser, J.
Resistance phenotypes mediated by aminoacyl-phosphatidylglycerol synthases
J. Bacteriol.
194
1401-1416
2012
Pseudomonas aeruginosa
brenda
Hebecker, S.; Arendt, W.; Heinemann, I.U.; Tiefenau, J.H.; Nimtz, M.; Rohde, M.; Soell, D.; Moser, J.
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa
Mol. Microbiol.
80
935-950
2011
Pseudomonas aeruginosa
brenda
Smith, A.; Harrison, J.; Grube, C.; Sheppe, A.; Sahara, N.; Ishii, R.; Nureki, O.; Roy, H.
tRNA-dependent alanylation of diacylglycerol and phosphatidylglycerol in Corynebacterium glutamicum
Mol. Microbiol.
98
681-693
2015
Corynebacterium glutamicum
brenda
Hebecker, S.; Krausze, J.; Hasenkampf, T.; Schneider, J.; Groenewold, M.; Reichelt, J.; Jahn, D.; Heinz, D.W.; Moser, J.
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Proc. Natl. Acad. Sci. USA
112
10691-10696
2015
Pseudomonas aeruginosa (Q9I537), Pseudomonas aeruginosa
brenda
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