HOME
login
history
all enzymes
BRENDA home
History
Information on EC 2.3.2.10 - UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase
for references in articles please use BRENDA:EC2.3.2.10Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferaseIUBMB Comments
Also acts on L-seryl-tRNASer.
Specify your search results
Word Map
- 2.3.2.10
- peptidoglycan
- aminoacyl-trnas
- medicine
-
1,2,3-triazole
- microhelix
-
aminoacylated
- ala-trnaala
-
phosphoramidite
- triazole
- deoxycytidine
-
five-membered
- nucleoside
-
cycloadditions
-
alkynes
-
regioisomers
The enzyme appears in viruses and cellular organisms
Synonyms
alanyl-transfer ribonucleate-uridine diphosphoacetylmuramoylpentapeptide transferase, alanyltransferase, alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-, FemX, FemXWv, FemXWv transferase, UDP-N-acetylmuramoylpentapeptide lysine N6-alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-alanyltransferase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alanyl-transfer ribonucleate-uridine diphosphoacetylmuramoylpentapeptide transferase
-
-
-
-
alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-
-
-
-
-
FemX
UDP-N-acetylmuramoylpentapeptide lysine N6-alanyltransferase
-
-
-
-
uridine diphosphoacetylmuramoylpentapeptide lysine N6-alanyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
also acts on L-seryl-tRNA
-
-
-
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
transfer of L-Ala to epsilon-amino group of L-lysine of UDP-UDP-acetylmuramoylpentapeptide in an ordered sequential mechanism
-
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
catalysis via a ternary complex
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
aminoacyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
L-alanyl-tRNAAla:UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine N6-alanyltransferase
Also acts on L-seryl-tRNASer.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37257-26-4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
glycyl-tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(glycyl)-L-lysyl-D-alanyl-D-alanine
-
poor substrate
-
-
ir
L-alanyl-tRNA(Ala) + UDP-N-acetylmuramoyl-pentapeptide containing L-lysine
?
-
-
-
-
r
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-cysteinyl-tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-cysteinyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
ir
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNA + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
-
about half as effective as L-alanyl-tRNA
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
poor or no acceptors are acetylmuramoyl- and phosphoacetylmuramoyl-L-Ala-L-Glu-L-Lys-D-Ala-D-Ala or UDP-acetylmuramoyl-L-Ala-L-Glu-L-Lys
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
no strict specificity for tRNA-carrier: tRNACys can replace tRNAAla, tRNA from other species can substitute to some extent for the homologous tRNA from Lactobacillus viridescens, higher specificity for transferred amino acid: amino acids with larger substituents are no substrates, UDP-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala can replace UDP-acetylmuramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala at 15% the transfer rate
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
involved in peptidoglucan metabolism, responsible for interpeptide bridges
-
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
production of precursors for branched chain peptidoglycans
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
production of precursors for branched chain peptidoglycans, involved in the synthesis of bacterial cell walls
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Weissella viridescens CIP 102810 / ATCC 12706
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Weissella viridescens CIP 102810 / ATCC 12706
-
production of precursors for branched chain peptidoglycans
-
-
?
additional information
?
-
-
also utilizes UDP-N-acetylmuramoylpentapeptide containing L-ornithine as substrate
-
-
?
additional information
?
-
-
enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide
-
-
?
additional information
?
-
-
catalyses the transfer of L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide in order to introduce the first residue of an L-Ala-L-Ser-L-Ala side chain
-
-
?
additional information
?
-
-
FemXWv transferase adds the first residue of the L-Ala-L-Ser-L-Ala side chain onto the nucleotide precursor UDP-MurNAc-pentadepsipeptide
-
-
?
additional information
?
-
Weissella viridescens CIP 102810 / ATCC 12706
-
enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
involved in peptidoglucan metabolism, responsible for interpeptide bridges
-
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
production of precursors for branched chain peptidoglycans
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
production of precursors for branched chain peptidoglycans, involved in the synthesis of bacterial cell walls
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Weissella viridescens CIP 102810 / ATCC 12706
-
production of precursors for branched chain peptidoglycans
-
-
?
additional information
?
-
-
catalyses the transfer of L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide in order to introduce the first residue of an L-Ala-L-Ser-L-Ala side chain
-
-
?
additional information
?
-
-
FemXWv transferase adds the first residue of the L-Ala-L-Ser-L-Ala side chain onto the nucleotide precursor UDP-MurNAc-pentadepsipeptide
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
product inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.079
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 30°C
0.015
L-alanyl-tRNA(Ala)
-
Ala-tRNAAla from E. coli, recombinant wild-type
0.054
L-alanyl-tRNA(Ala)
-
Ala-tRNAAla from E. coli, recombinant mutant E320Q
0.061
L-alanyl-tRNA(Ala)
-
Ala-tRNAAla from E. coli, recombinant mutant D109N
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.048
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
product inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
relative activity of FemXWv for the transfer of L-Ala from helixAla to UDP-MurNAc-pentapeptide 100%
additional information
-
relative activity of FemXWv for the transfer of L-Ala from helixGly to UDP-MurNAc-pentapeptide 2.9%
additional information
-
relative activity of FemXWv for the transfer of L-Ala from helixSer to UDP-MurNAc-pentapeptide 31%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.2 - 8.6
-
L-alanyl-tRNA, about 80% of maximal activity at pH 5.2 and pH 8.6
6.1 - 8.6
-
L-seryl-tRNA, about half-maximal activity at pH 6.1 and about 90% of maximal activity at pH 8.6
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q48UK2_STRPM
Streptococcus pyogenes serotype M28 (strain MGAS6180)
413
0
46692
TrEMBL
-
F5WVQ4_STRG1
Streptococcus gallolyticus (strain ATCC 43143 / F-1867)
404
1
46424
TrEMBL
-
K4Q969_STREQ
180
0
20662
TrEMBL
-
A0A644ZCE1_9ZZZZ
383
0
42911
TrEMBL
other Location (Reliability: 5)
M4YWQ5_STREQ
407
0
46634
TrEMBL
-
K4Q8H5_STREQ
405
0
46272
TrEMBL
-
A9QSG8_LACLK
Lactococcus lactis subsp. lactis (strain KF147)
399
0
46477
TrEMBL
-
Q1J6G4_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
407
0
47279
TrEMBL
-
Q5XC19_STRP6
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
407
0
47307
TrEMBL
-
Q1JMR0_STRPC
Streptococcus pyogenes serotype M12 (strain MGAS9429)
413
0
46559
TrEMBL
-
A0A645JHE0_9ZZZZ
163
0
19199
TrEMBL
other Location (Reliability: 1)
A0A344UQD6_9ACTN
388
0
43684
TrEMBL
-
Q1JHV3_STRPD
Streptococcus pyogenes serotype M2 (strain MGAS10270)
413
0
46521
TrEMBL
-
C5WGD1_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
407
0
46634
TrEMBL
-
K4Q876_STREQ
226
0
25795
TrEMBL
-
G6ENS4_STRTR
404
0
46210
TrEMBL
-
F5X2D3_STRPX
Streptococcus pasteurianus (strain ATCC 43144 / JCM 5346 / CDC 1723-81)
404
0
46287
TrEMBL
-
Q1JGP5_STRPD
Streptococcus pyogenes serotype M2 (strain MGAS10270)
407
0
47336
TrEMBL
-
Q48TF5_STRPM
Streptococcus pyogenes serotype M28 (strain MGAS6180)
407
0
47341
TrEMBL
-
Q1JLL0_STRPC
Streptococcus pyogenes serotype M12 (strain MGAS9429)
407
0
47292
TrEMBL
-
C5WFH4_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
410
0
46785
TrEMBL
-
Q1J7N1_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
413
0
46565
TrEMBL
-
Q02W53_LACLS
Lactococcus lactis subsp. cremoris (strain SK11)
396
0
46224
TrEMBL
-
A0A101RUC5_9ACTN
373
0
42981
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallized in the presence of UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine by vapor diffusion method
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D109N
-
site-directed mutagenesis, loss of 99% activity compared to the wild-type
E205Q
-
site-directed mutagenesis, unaltered activity compared to the wild-type
E215Q
-
site-directed mutagenesis, loss of 15% activity compared to the wild-type
E316Q
-
site-directed mutagenesis, loss of 60% activity compared to the wild-type
E320Q
-
site-directed mutagenesis, loss of 96% activity compared to the wild-type
K36R
-
160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
R211K
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
Y215F
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
R211K
Weissella viridescens CIP 102810 / ATCC 12706
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
-
Y215F
Weissella viridescens CIP 102810 / ATCC 12706
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of wild-type and mutants as His-tagged proteins in Escherichia coli, in vitro transcription of tRNAs
-
wild type and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli TOP10
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
medicine
-
Fem transferases are considered as attractive targets for the development of novel antibiotics active against multi-resistant bacteria
medicine
-
Fem transferases are considered attractive targets for the development of novel antibiotics active against multiply resistant bacteria
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Plapp, R.; Strominger, J.L.
Biosynthesis of the peptidoglycan of bacterial cell walls
J. Biol. Chem.
245
3673-3682
1970
Weissella viridescens
-
brenda
Hegde, S.S.; Blanchard, J.S.
Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)
J. Biol. Chem.
278
22861-22867
2003
Weissella viridescens
brenda
Maillard, A.P.; Biarrotte-Sorin, S.; Villet, R.; Mesnage, S.; Bouhss, A.; Sougakoff, W.; Mayer, C.; Arthur, M.
Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescens
J. Bacteriol.
187
3833-3838
2005
Weissella viridescens, Weissella viridescens CIP 102810 / ATCC 12706
brenda
Biarrotte-Sorin, S.; Maillard, A.P.; Delettre, J.; Sougakoff, W.; Arthur, M.; Mayer, C.
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: Insights into FemABX family substrates recognition
Structure
12
257-267
2004
Weissella viridescens (Q9EY50)
brenda
Chemama, M.; Fonvielle, M.; Arthur, M.; Valery, J.M.; Etheve-Quelquejeu, M.
Synthesis of stable aminoacyl-tRNA analogues containing triazole as a bioisoster of esters
Chemistry
15
1929-1938
2009
Weissella viridescens
brenda
Fonvielle, M.; Chemama, M.; Villet, R.; Lecerf, M.; Bouhss, A.; Valery, J.M.; Etheve-Quelquejeu, M.; Arthur, M.
Aminoacyl-tRNA recognition by the FemXWv transferase for bacterial cell wall synthesis
Nucleic Acids Res.
37
1589-1601
2009
Weissella viridescens
brenda
Mellal, D.; Fonvielle, M.; Santarem, M.; Chemama, M.; Schneider, Y.; Iannazzo, L.; Braud, E.; Arthur, M.; Etheve-Quelquejeu, M.
Synthesis and biological evaluation of non-isomerizable analogues of Ala-tRNAAla
Org. Biomol. Chem.
11
6161-6169
2013
Weissella viridescens
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 2.3.2.10)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Information
