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Information on EC 2.3.2.10 - UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase
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EC Tree
2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferaseIUBMB Comments
Also acts on L-seryl-tRNASer.
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Word Map
- 2.3.2.10
- peptidoglycan
- aminoacyl-trnas
- medicine
-
cycloadditions
- microhelix
-
five-membered
- triazole
- deoxycytidine
-
1,2,3-triazole
-
aminoacylated
- nucleoside
- regioisomers
-
alkynes
-
phosphoramidite
- ala-trnaala
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
femxwv, alanyltransferase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alanyl-transfer ribonucleate-uridine diphosphoacetylmuramoylpentapeptide transferase
-
-
-
-
alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-
-
-
-
-
FemX
UDP-N-acetylmuramoylpentapeptide lysine N6-alanyltransferase
-
-
-
-
uridine diphosphoacetylmuramoylpentapeptide lysine N6-alanyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
also acts on L-seryl-tRNA
-
-
-
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
transfer of L-Ala to epsilon-amino group of L-lysine of UDP-UDP-acetylmuramoylpentapeptide in an ordered sequential mechanism
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L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
catalysis via a ternary complex
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
aminoacyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
L-alanyl-tRNAAla:UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine N6-alanyltransferase
Also acts on L-seryl-tRNASer.
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CAS REGISTRY NUMBER
COMMENTARY
37257-26-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
glycyl-tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(glycyl)-L-lysyl-D-alanyl-D-alanine
-
poor substrate
-
-
ir
L-alanyl-tRNA(Ala) + UDP-N-acetylmuramoyl-pentapeptide containing L-lysine
?
-
-
-
-
r
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-cysteinyl-tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-cysteinyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
ir
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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poor or no acceptors are acetylmuramoyl- and phosphoacetylmuramoyl-L-Ala-L-Glu-L-Lys-D-Ala-D-Ala or UDP-acetylmuramoyl-L-Ala-L-Glu-L-Lys
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ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
no strict specificity for tRNA-carrier: tRNACys can replace tRNAAla, tRNA from other species can substitute to some extent for the homologous tRNA from Lactobacillus viridescens, higher specificity for transferred amino acid: amino acids with larger substituents are no substrates, UDP-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala can replace UDP-acetylmuramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala at 15% the transfer rate
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
involved in peptidoglucan metabolism, responsible for interpeptide bridges
-
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
production of precursors for branched chain peptidoglycans
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
production of precursors for branched chain peptidoglycans, involved in the synthesis of bacterial cell walls
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Weissella viridescens CIP 102810 / ATCC 12706
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Weissella viridescens CIP 102810 / ATCC 12706
-
production of precursors for branched chain peptidoglycans
-
-
?
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
-
-
-
?
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
-
about half as effective as L-alanyl-tRNA
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ir
additional information
?
-
-
also utilizes UDP-N-acetylmuramoylpentapeptide containing L-ornithine as substrate
-
-
?
additional information
?
-
-
enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide
-
-
?
additional information
?
-
-
catalyses the transfer of L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide in order to introduce the first residue of an L-Ala-L-Ser-L-Ala side chain
-
-
?
additional information
?
-
-
FemXWv transferase adds the first residue of the L-Ala-L-Ser-L-Ala side chain onto the nucleotide precursor UDP-MurNAc-pentadepsipeptide
-
-
?
additional information
?
-
Weissella viridescens CIP 102810 / ATCC 12706
-
enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
-
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
involved in peptidoglucan metabolism, responsible for interpeptide bridges
-
-
ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
production of precursors for branched chain peptidoglycans
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
production of precursors for branched chain peptidoglycans, involved in the synthesis of bacterial cell walls
-
-
?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Weissella viridescens CIP 102810 / ATCC 12706
-
production of precursors for branched chain peptidoglycans
-
-
?
additional information
?
-
-
catalyses the transfer of L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide in order to introduce the first residue of an L-Ala-L-Ser-L-Ala side chain
-
-
?
additional information
?
-
-
FemXWv transferase adds the first residue of the L-Ala-L-Ser-L-Ala side chain onto the nucleotide precursor UDP-MurNAc-pentadepsipeptide
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
product inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.079
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 30°C
0.015
L-alanyl-tRNA(Ala)
-
Ala-tRNAAla from E. coli, recombinant wild-type
0.054
L-alanyl-tRNA(Ala)
-
Ala-tRNAAla from E. coli, recombinant mutant E320Q
0.061
L-alanyl-tRNA(Ala)
-
Ala-tRNAAla from E. coli, recombinant mutant D109N
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.048
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
product inhibition
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
relative activity of FemXWv for the transfer of L-Ala from helixAla to UDP-MurNAc-pentapeptide 100%
additional information
-
relative activity of FemXWv for the transfer of L-Ala from helixGly to UDP-MurNAc-pentapeptide 2.9%
additional information
-
relative activity of FemXWv for the transfer of L-Ala from helixSer to UDP-MurNAc-pentapeptide 31%
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.2 - 8.6
-
L-alanyl-tRNA, about 80% of maximal activity at pH 5.2 and pH 8.6
6.1 - 8.6
-
L-seryl-tRNA, about half-maximal activity at pH 6.1 and about 90% of maximal activity at pH 8.6
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A344UQD6_9ACTN
388
0
43684
TrEMBL
-
C5WGD1_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
407
0
46634
TrEMBL
-
M4YWQ5_STREQ
407
0
46634
TrEMBL
-
C5WFH4_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
410
0
46785
TrEMBL
-
F5WVQ4_STRG1
Streptococcus gallolyticus (strain ATCC 43143 / F-1867)
404
1
46424
TrEMBL
-
A9QSG8_LACLK
Lactococcus lactis subsp. lactis (strain KF147)
399
0
46477
TrEMBL
-
Q1J6G4_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
407
0
47279
TrEMBL
-
Q1JLL0_STRPC
Streptococcus pyogenes serotype M12 (strain MGAS9429)
407
0
47292
TrEMBL
-
Q1JMR0_STRPC
Streptococcus pyogenes serotype M12 (strain MGAS9429)
413
0
46559
TrEMBL
-
Q48TF5_STRPM
Streptococcus pyogenes serotype M28 (strain MGAS6180)
407
0
47341
TrEMBL
-
Q1JHV3_STRPD
Streptococcus pyogenes serotype M2 (strain MGAS10270)
413
0
46521
TrEMBL
-
Q02W53_LACLS
Lactococcus lactis subsp. cremoris (strain SK11)
396
0
46224
TrEMBL
-
Q1JGP5_STRPD
Streptococcus pyogenes serotype M2 (strain MGAS10270)
407
0
47336
TrEMBL
-
A0A8A0RK29_9FIRM
362
0
42200
TrEMBL
-
A0A644ZCE1_9ZZZZ
383
0
42911
TrEMBL
other Location (Reliability: 5)
A0A7W8FW43_9FIRM
410
0
48243
TrEMBL
-
Q48UK2_STRPM
Streptococcus pyogenes serotype M28 (strain MGAS6180)
413
0
46692
TrEMBL
-
F5X2D3_STRPX
Streptococcus pasteurianus (strain ATCC 43144 / JCM 5346 / CDC 1723-81)
404
0
46287
TrEMBL
-
G6ENS4_STRTR
404
0
46210
TrEMBL
-
A0A101RUC5_9ACTN
373
0
42981
TrEMBL
-
Q1J7N1_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
413
0
46565
TrEMBL
-
Q5XC19_STRP6
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
407
0
47307
TrEMBL
-
A0A645JHE0_9ZZZZ
163
0
19199
TrEMBL
other Location (Reliability: 1)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallized in the presence of UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine by vapor diffusion method
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D109N
-
site-directed mutagenesis, loss of 99% activity compared to the wild-type
E205Q
-
site-directed mutagenesis, unaltered activity compared to the wild-type
E215Q
-
site-directed mutagenesis, loss of 15% activity compared to the wild-type
E316Q
-
site-directed mutagenesis, loss of 60% activity compared to the wild-type
E320Q
-
site-directed mutagenesis, loss of 96% activity compared to the wild-type
K36R
-
160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
R211K
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
Y215F
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
R211K
Weissella viridescens CIP 102810 / ATCC 12706
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
-
Y215F
Weissella viridescens CIP 102810 / ATCC 12706
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-