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Information on EC 2.3.1.87 - aralkylamine N-acetyltransferase
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2.3.1.87 aralkylamine N-acetyltransferaseIUBMB Comments
Narrow specificity towards 2-arylethylamines, including serotonin (5-hydroxytryptamine), tryptamine, 5-methoxytryptamine and phenylethylamine. This is the penultimate enzyme in the production of melatonin (5-methoxy-N-acetyltryptamine) and controls its synthesis (cf. EC 2.1.1.4, acetylserotonin O-methyltransferase). Differs from EC 2.3.1.5 arylamine N-acetyltransferase.
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Word Map
- 2.3.1.87
- melatonin
-
pineal
- rhythm
-
circadian
- clock
- retina
-
night
-
nocturnal
- norepinephrine
- hiomt
- pinealocytes
-
arylalkylamine-n-acetyltransferase
- hydroxyindole-o-methyltransferase
- photoreceptors
-
oscillator
- n-acetylserotonin
-
diurnal
-
suprachiasmatic
-
photoperiodic
-
snats
-
light-dark
-
sodium-coupled
-
adrenergic
- tryptamine
-
entrain
-
photoreceptive
- synthesis
- n-acetyl-5-methoxytryptamine
-
gcn5-related
-
photic
-
zeitgeber
-
clockwork
-
day-night
- octopamine
-
night-time
-
clock-controlled
- medicine
- analysis
-
ne-stimulated
-
hydroxyindole
-
meaib
-
melatoninergic
-
rhythm-generating
-
extrapineal
-
slc38a2
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
AA-NAT, AANAT, AANAT1, AANAT2, AANAT3, AANATL7, AATNATL2, acetyl-CoA:aralkylamine N-acetyltransferase, acetyltransferase, arylalkylamine N-, arylalkylamine N-acetyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AA-NAT
AANAT
AANAT1
AANAT2
acetyl-CoA:aralkylamine N-acetyltransferase
-
-
-
-
acetyltransferase, arylalkylamine N-
-
-
-
-
arylalkylamine N-acetyltransferase
arylalkylamine N-acetyltransferase 1
arylalkylamine N-acetyltransferase 2
arylalkylamine N-acetyltransferase-2
Eis
MSMEG_3513
N-acetyltransferase
-
-
-
-
NAT
oAANAT
PA4534
Rv2416c
serotonin acetylase
serotonin acetyltransferase
-
-
-
-
serotonin N-acetyltransferase
SNAT
SNAT2
additional information
AA-NAT
-
-
-
-
AANAT
-
-
-
-
arylalkylamine N-acetyltransferase
-
-
-
-
serotonin acetylase
-
-
-
-
additional information
AANAT is a member of the GCN5-related N-acetyltransferase, GNAT, superfamily of acetyltransferases
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
mechanism
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
ordered bi-bi sequential mechanism
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
catalytic reaction mechanism, overview
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
mechanism
-
-
acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:2-arylethylamine N-acetyltransferase
Narrow specificity towards 2-arylethylamines, including serotonin (5-hydroxytryptamine), tryptamine, 5-methoxytryptamine and phenylethylamine. This is the penultimate enzyme in the production of melatonin (5-methoxy-N-acetyltryptamine) and controls its synthesis (cf. EC 2.1.1.4, acetylserotonin O-methyltransferase). Differs from EC 2.3.1.5 arylamine N-acetyltransferase.
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CAS REGISTRY NUMBER
COMMENTARY
92941-56-5
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 2,5-dimethoxyphenylethylamine
CoA + ?
24% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2,5-dimethoxyphenylethylamine
CoA + N-acetyl-2,5-dimethoxyphenylethylamine
-
68% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine
CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine
CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine
CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
acetyl-CoA + 2-fluorophenylethylamine
CoA + ?
69% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-fluorophenylethylamine
CoA + N-acetyl-2-fluorophenylethylamine
-
52% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-(trifluoromethyl)phenethylamine
CoA + N-acetyl-3-(trifluoromethyl)phenethylamine
-
-
-
-
?
acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine
CoA + ?
1% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine
CoA + N-acetyl-3-hydroxy-4-methoxyphenethylamine
-
7.2% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-hydroxyphenethylamine
CoA + ?
24% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-hydroxyphenethylamine
CoA + N-acetyl-3-hydroxyphenethylamine
-
36% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-indolebutylamine
CoA + N-acetyl-(3-indol-3-yl-butyl)-amine
-
60-fold less efficiently than serotonin
-
-
?
acetyl-CoA + 3-indolepropylamine
CoA + N-acetyl-(3-indol-3-yl-propyl)-amine
-
20-fold less efficiently than serotonin
-
-
?
acetyl-CoA + 3-methoxy-2-phenylethylamine
CoA + ?
23% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-methoxy-2-phenylethylamine
CoA + N-acetyl-3-methoxy-2-phenylethylamine
-
55% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-methoxyphenethylamine
CoA + N-acetyl-3-methoxyphenethylamine
-
-
-
-
?
acetyl-CoA + 3-methoxytyramine
CoA + ?
17% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 3-methoxytyramine
CoA + N-acetyl-3-methoxytyramine
-
73% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonamide
CoA + ?
-
1% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 5-benzyloxytryptamine
CoA + N-acetyl-5-benzyloxytryptamine
-
-
-
-
?
acetyl-CoA + 5-hydroxydopamine
CoA + N-acetyl-5-hydroxydopamine
-
4.4% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 6-hydroxydopamine
CoA + ?
51% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 6-hydroxydopamine
CoA + N-acetyl-6-hydroxydopamine
-
1.5% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + alpha-methyltryptamine
CoA + N-acetyl-alpha-methyltryptamine
-
racemic, 9:1 stereoselectivity for R-enantiomer, less efficiently than serotonin
-
-
?
acetyl-CoA + beta-methylphenethylamine
CoA + N-acetyl-beta-methylphenethylamine
-
-
-
-
?
acetyl-CoA + Nomega-methyltryptamine
CoA + ?
-
less efficiently than serotonin
-
-
?
acetyl-CoA + tryptophol
CoA + N-acetyltryptophol
-
structural analogue to tryptamine
-
-
?
acetyl-CoA + tyramine
CoA + ?
8% of the activity with 2-phenylethylamine
-
-
?
arachidonoyl-CoA + octopamine
CoA + N-arachidonoyloctopamine
-
-
-
?
palmitoyl-CoA + octopamine
CoA + N-palmitoyloctopamine
-
-
-
?
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine
CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
78% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine
CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
-
84% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(2-chlorophenyl)-ethylamine
CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine
130% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(2-chlorophenyl)-ethylamine
CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine
-
102% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine
CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
7% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine
CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
-
64% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine
CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
4.4% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine
CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
-
15% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3-chlorophenyl)-ethylamine
CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine
67% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3-chlorophenyl)-ethylamine
CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine
-
110% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3-fluorophenyl)-ethylamine
CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine
65% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(3-fluorophenyl)-ethylamine
CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine
-
93% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(4-bromophenyl)-ethylamine
CoA + N-acetyl-2-(4-bromophenyl)-ethylamine
48% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(4-bromophenyl)-ethylamine
CoA + N-acetyl-2-(4-bromophenyl)-ethylamine
-
87% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(4-chlorophenyl)ethylamine
CoA + N-acetyl-2-(4-chlorophenyl)ethylamine
68% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(4-chlorophenyl)ethylamine
CoA + N-acetyl-2-(4-chlorophenyl)ethylamine
-
91% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(4-fluorophenyl)-ethylamine
CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine
116% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(4-fluorophenyl)-ethylamine
CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine
-
60% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(p-fluorophenyl)-ethylamine
CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine
56% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(p-fluorophenyl)-ethylamine
CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine
-
as active as with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(p-nitrophenyl)-ethylamine
CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine
9% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(p-nitrophenyl)-ethylamine
CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine
-
53% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(p-tolyl)-ethylamine
CoA + N-acetyl-2-(p-tolyl)-ethylamine
60% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-(p-tolyl)-ethylamine
CoA + N-acetyl-2-(p-tolyl)-ethylamine
-
87% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-methoxyphenylethylamine
CoA + N-acetyl-2-methoxyphenylethylamine
52% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-methoxyphenylethylamine
CoA + N-acetyl-2-methoxyphenylethylamine
-
97% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 2-phenylethylamine
CoA + N-acetyl-2-phenylethylamine
-
-
-
?
acetyl-CoA + 2-phenylethylamine
CoA + N-acetyl-2-phenylethylamine
-
-
-
?
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride
CoA + ?
8% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride
CoA + ?
-
7.4% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 4-methoxyphenylethylamine
CoA + N-acetyl-4-methoxyphenylethylamine
11% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 4-methoxyphenylethylamine
CoA + N-acetyl-4-methoxyphenylethylamine
-
10% of the activity with 2-phenylethylamine
-
-
?
acetyl-CoA + 5-hydroxytryptamine
CoA + N-acetyl-5-hydroxytryptamine
-
-
-
-
?
acetyl-CoA + 5-hydroxytryptamine
CoA + N-acetyl-5-hydroxytryptamine
-
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
-
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
-
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
-
-
-
-
?
acetyl-CoA + 6-fluorotryptamine
CoA + N-acetyl-6-fluorotryptamine
-
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
-
also substrate: phenylethylamine derivatives without a beta-hydroxy group
-
-
?
acetyl-CoA + dopamine
CoA + N-acetyldopamine
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
-
-
-
?
acetyl-CoA + norepinephrine
CoA + N-acetylnorepinephrine
-
-
-
?
acetyl-CoA + octopamine
CoA + N-acetyloctopamine
-
-
-
?
acetyl-CoA + octopamine
CoA + N-acetyloctopamine
-
-
-
?
acetyl-CoA + phenylethylamine
CoA + N-acetylphenylethylamine
-
-
-
?
acetyl-CoA + phenylethylamine
CoA + N-acetylphenylethylamine
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
i.e. 5-hydroxytryptamine
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
rate-limiting in melatonin, i.e. N-acetyl-5-methoxytryptamine synthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
best substrate
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
key enzyme of circadian rhythm of melatonin synthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
rate-limiting in melatonin synthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
the enzyme catalyzes the first step in melatonin biosynthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
i.e. 5-hydroxytryptamine
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
i.e. 5-hydroxytryptamine
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
initial reaction in melatonin synthesis from serotonin
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
penultimate enzyme in melatonin pathway
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
penultimate step in melatonin biosynthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
reaction mechanism involving Pro64 that plays a critical role in structure and catalysis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
i.e. 5-hydroxytryptamine
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
i.e. 5-hydroxytryptamine
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
i.e. 5-hydroxytryptamine
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
initial reaction in melatonin synthesis from serotonin
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
first step in melatonin biosynthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
AANAT2 acetylates serotonin about 10times faster than AANAT1
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
-
-
-
?
acetyl-CoA + tyramine
CoA + N-acetyltyramine
-
-
-
?
acetyl-CoA + tyramine
CoA + N-acetyltyramine
-
10% of the activity with 2-phenylethylamine
-
-
?
propionyl-CoA + histamine
CoA + N-propionylhistamine
-
-
-
?
propionyl-CoA + histamine
CoA + N-propionylhistamine
-
-
-
?
propionyl-CoA + histamine
CoA + N-propionylhistamine
-
-
-
?
propionyl-CoA + histamine
CoA + N-propionylhistamine
-
-
-
?
propionyl-CoA + tyramine
CoA + N-propionyltyramine
-
-
-
?
propionyl-CoA + tyramine
CoA + N-propionyltyramine
-
-
-
?
additional information
?
-
aaNAT2, a protein from the typical insect aaNAT cluster, uses histamine as a substrate as well as arylalkylamines
-
-
?
additional information
?
-
-
aaNAT2, a protein from the typical insect aaNAT cluster, uses histamine as a substrate as well as arylalkylamines
-
-
?
additional information
?
-
-
substrate specificity, aaNAT1 and aaNAT2 show a broad substrate specificity and their affinity and catalytic efficiency to each of the seven arylalkylamines tested. The affinity between aaNAT1 and aaNAT2 to most of the arylalkylamines is similar except that aaNAT2 has less affinity than aaNAT1 to norepinephrine. Also aaNAT1 is more efficient in catalyzing all the tested substrates than that of aaNAT2
-
-
?
additional information
?
-
-
ordered sequential mechanism, with acetyl-CoA binding first followed by histamine to generate an AANATL7-acetyl-CoA-histamine ternary complex prior to catalysis. An ionizable group with a pKa of 7.1 is assigned to Glu-26 as a general base and a second with a pKa of 9.5 is assigned to the protonation of the thiolate of the coenzyme A product
-
-
?
additional information
?
-
-
enzyme activity in cultured cells is suppressed by light, as it is in vivo. The ability to express circadian regulation of the enzyme activity is an intrinsic property of retinal cells that can develop in vitro
-
-
?
additional information
?
-
-
further substrates: selected synthetic amines
-
-
?
additional information
?
-
-
the enzyme catalyzes the rate-limiting step in melatonin synthesis
-
-
?
additional information
?
-
no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine, 5-hydroxydopamine, 4-(2-aminoethyl)-benzenesulfonamide or 2-(p-chlorophenoxy)-2-methylpropionic acid
-
-
?
additional information
?
-
-
no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine, 5-hydroxydopamine, 4-(2-aminoethyl)-benzenesulfonamide or 2-(p-chlorophenoxy)-2-methylpropionic acid
-
-
?
additional information
?
-
-
enzyme light and diurnal regulation involves phosphorylation on key AANAT Ser and Thr residues, which results in 14-3-3 protein recruitment and changes in catalytic activity and protein stability
-
-
?
additional information
?
-
the enzyme catalyzes the limiting step for melatonin synthesis
-
-
?
additional information
?
-
-
the enzyme catalyzes the limiting step for melatonin synthesis
-
-
?
additional information
?
-
-
arylamines, such as aniline or p-phenetidine are very poor substrates
-
-
?
additional information
?
-
-
arylamines, such as aniline or p-phenetidine are very poor substrates
-
-
?
additional information
?
-
-
no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine or 2-(p-chlorophenoxy)-2-methylpropionic acid
-
-
?
additional information
?
-
-
NAT could be a clock-controlled gene functioning as an output regulator of the circadian clock
-
-
?
additional information
?
-
-
no substrates are phenylethanolamine derivatives with a beta-hydroxy group
-
-
?
additional information
?
-
-
arylamines, such as aniline or p-phenetidine are very poor substrates
-
-
?
additional information
?
-
-
arylamines, such as aniline or p-phenetidine are very poor substrates
-
-
?
additional information
?
-
overexpression of inducible cAMP early repressor can suppress the norepinephrine induction of aa-nat
-
-
?
additional information
?
-
AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin
-
-
?
additional information
?
-
AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin
-
-
?
additional information
?
-
-
AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin
-
-
?
additional information
?
-
AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role
-
-
?
additional information
?
-
AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role
-
-
?
additional information
?
-
-
AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role
-
-
?
additional information
?
-
insignificant activity in presence of phenylethylamine or tyramine
-
-
?
additional information
?
-
insignificant activity in presence of phenylethylamine or tyramine
-
-
?
additional information
?
-
-
insignificant activity in presence of phenylethylamine or tyramine
-
-
?
additional information
?
-
-
AANAT is a key regulatory enzyme in the melatonin biosynthetic pathway
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
-
?
acetyl-CoA + a 2-arylethylamine
CoA + an N-acetyl-2-arylethylamine
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
rate-limiting in melatonin, i.e. N-acetyl-5-methoxytryptamine synthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
key enzyme of circadian rhythm of melatonin synthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
rate-limiting in melatonin synthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
the enzyme catalyzes the first step in melatonin biosynthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
initial reaction in melatonin synthesis from serotonin
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
penultimate enzyme in melatonin pathway
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
penultimate step in melatonin biosynthesis
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
initial reaction in melatonin synthesis from serotonin
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
-
first step in melatonin biosynthesis
-
-
?
additional information
?
-
aaNAT2, a protein from the typical insect aaNAT cluster, uses histamine as a substrate as well as arylalkylamines
-
-
?
additional information
?
-
-
aaNAT2, a protein from the typical insect aaNAT cluster, uses histamine as a substrate as well as arylalkylamines
-
-
?
additional information
?
-
-
enzyme activity in cultured cells is suppressed by light, as it is in vivo. The ability to express circadian regulation of the enzyme activity is an intrinsic property of retinal cells that can develop in vitro
-
-
?
additional information
?
-
-
the enzyme catalyzes the rate-limiting step in melatonin synthesis
-
-
?
additional information
?
-
-
enzyme light and diurnal regulation involves phosphorylation on key AANAT Ser and Thr residues, which results in 14-3-3 protein recruitment and changes in catalytic activity and protein stability
-
-
?
additional information
?
-
the enzyme catalyzes the limiting step for melatonin synthesis
-
-
?
additional information
?
-
-
the enzyme catalyzes the limiting step for melatonin synthesis
-
-
?
additional information
?
-
-
NAT could be a clock-controlled gene functioning as an output regulator of the circadian clock
-
-
?
additional information
?
-
overexpression of inducible cAMP early repressor can suppress the norepinephrine induction of aa-nat
-
-
?
additional information
?
-
AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin
-
-
?
additional information
?
-
AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin
-
-
?
additional information
?
-
-
AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin
-
-
?
additional information
?
-
AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role
-
-
?
additional information
?
-
AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role
-
-
?
additional information
?
-
-
AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role
-
-
?
additional information
?
-
-
AANAT is a key regulatory enzyme in the melatonin biosynthetic pathway
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
activation by salts as a function of ionic strength
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9-carboxy-11-nor-delta-9-tetrahydrocannabinol
significantly reduces norepinephrine-induced arylalkylamine N-acetyltransferase activity
cannabidiol
significantly reduces norepinephrine-induced arylalkylamine N-acetyltransferase activity
cannabinoid
cannabinoids inhibit AANAT activity and attenuate melatonin biosynthesis through intracellular actions without involvement of classical cannabinoid receptor-dependent signaling cascades
cannabinol
significantly reduces norepinephrine-induced arylalkylamine N-acetyltransferase activity
catechin
-
poorest inhibitor tested, 0.1 mM, 1.6fold inhibition of isoform SNAT1 at 0.1 mM
CoA-S-N-acetyl-7-hydroxynaphthylethylamine
fluorescent variant of CoA-T, a bisubstrate inhibitor, synthesis and enzyme binding structure, molecular modelling, overview
CoA-S-N-acetyltryptamine
total inhibition of N-acetyldopamine synthesis in presence of acetyl coenzyme-A and dopamine occurs in the presence of CoA-S-N-acetyltryptamine in liver
methyl 3-[2-[(bromoacetyl)amino]ethyl]-1-benzothiophene-5-carboxylate
-
IC50: 0.00225 mM
N-[2-(7-ethyl-1,2,3,4-tetrahydronapht-1-yl)ethyl]iodooacetamide
-
IC50: above 0.1 mM
N-[2-(7-methoxy-3-(3-trifluoromethylphenyl)-napht-1-yl)ethyl]iodoacetamide
-
IC50: 0.045 mM
tryptamine-coenzyme A
a bisubstrate inhibitor, enzyme binding structure, molecular modelling, overview
UV light
-
NAT activity and melatonin content are suppressed by blue light of 450 nm wavelength, no effects of red light at 650 nm.UV radiation has intensity-dependent dual effects on the NAT activity and melatonin content: it is supressing at low intensity and activating at high intensity irradiation, overview
-
additional information
both white and blue light pulses significantly inhibit AA-NAT activity at zeitgeber time ZT18, detailed overview
-
additional information
-
both white and blue light pulses significantly inhibit AA-NAT activity at zeitgeber time ZT18, detailed overview
-
additional information
no inhibition by serotonin or tryptamin above 1 mM. No inhibition by melatonin
-
additional information
no inhibition by serotonin or tryptamin above 1 mM. No inhibition by melatonin
-
additional information
-
no inhibition by serotonin or tryptamin above 1 mM. No inhibition by melatonin
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
14-3-3 protein
-
14-3-3zeta, interaction with the phosphorylated enzyme activates activity, binding analysis, overview
-
4beta-phorbol 12-myristate 13-acetate
-
stimulates activity on a posttranscriptional level in a cAMP-independent manner
leptin
-
increases enzyme activity only in fed fish not in fasted fish, independently of the seasons
-
UV light
-
NAT activity and melatonin content are suppressed by blue light of 450 nm wavelength, no effects of red light at 650 nm.UV radiation has intensity-dependent dual effects on the NAT activity and melatonin content: it is supressing at low intensity and activating at high intensity irradiation, overview
-
additional information
-
the enzyme is activated by Ser and Thr phosphorylations, e.g. AANAT Thr31 phosphorylation on its own can enhance catalytic efficiency up to 7fold
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Acidosis
Acidosis-sensing glutamine pump SNAT2 determines amino acid levels and mammalian target of rapamycin signalling to protein synthesis in L6 muscle cells.
Acidosis
Inhibition of SNAT2 by metabolic acidosis enhances proteolysis in skeletal muscle.
aralkylamine n-acetyltransferase deficiency
Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system.
aralkylamine n-acetyltransferase deficiency
Prolonged swim-test immobility of serotonin N-acetyltransferase (AANAT)-mutant mice.
Astrocytoma
Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas.
Brain Injuries
Up-regulation of miR-325-3p suppresses pineal aralkylamine N-acetyltransferase (Aanat) after neonatal hypoxia-ischemia brain injury in rats.
Brain Neoplasms
Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas.
Breast Neoplasms
Activation of SNAT1/SLC38A1 in human breast cancer: correlation with p-Akt overexpression.
Breast Neoplasms
Altered Umbilical Cord Blood Nutrient Levels, Placental Cell Turnover and Transporter Expression in Human Term Pregnancies Conceived by Intracytoplasmic Sperm Injection (ICSI).
Breast Neoplasms
Hypoxia-induced switch in SNAT2/SLC38A2 regulation generates endocrine resistance in breast cancer.
Breast Neoplasms
Polymorphisms in circadian genes, night work and breast cancer: results from the GENICA study.
Breast Neoplasms
SNAT2 transceptor signalling via mTOR: a role in cell growth and proliferation?
Carcinogenesis
Activation of SNAT1/SLC38A1 in human breast cancer: correlation with p-Akt overexpression.
Carcinogenesis
O-GlcNAcylation of YY1 stimulates tumorigenesis in colorectal cancer cells by targeting SLC22A15 and AANAT.
Carcinoma, Hepatocellular
Characterization of the amino acid response element within the human sodium-coupled neutral amino acid transporter 2 (SNAT2) System A transporter gene.
Carcinoma, Hepatocellular
Despite Increased ATF4 Binding at the C/EBP-ATF Composite Site following Activation of the Unfolded Protein Response, System A Transporter 2 (SNAT2) Transcription Activity Is Repressed in HepG2 Cells.
Choriocarcinoma
Human placental trophoblasts synthesize melatonin and express its receptors.
Chronobiology Disorders
The flavonoid myricetin reduces nocturnal melatonin levels in the blood through the inhibition of serotonin N-acetyltransferase.
Colorectal Neoplasms
AA-NAT, MT1 and MT2 Correlates with Cancer Stem-Like Cell Markers in Colorectal Cancer: Study of the Influence of Stage and p53 Status of Tumors.
Colorectal Neoplasms
Melatonin reduces endothelin-1 expression and secretion in colon cancer cells through the inactivation of FoxO-1 and NF-??
Colorectal Neoplasms
O-GlcNAcylation of YY1 stimulates tumorigenesis in colorectal cancer cells by targeting SLC22A15 and AANAT.
Endometrial Neoplasms
ASCT2 regulates glutamine uptake and cell growth in endometrial carcinoma.
Epilepsy
Modulation of epileptiform activity by glutamine and system A transport in a model of post-traumatic epilepsy.
Glioma
Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas.
Infections
Attacking the Supply Lines: HIV-1 Restricts Alanine Uptake to Prevent T Cell Activation.
Infections
Expression of Melatonin Synthesizing Enzymes in Helicobacter pylori Infected Gastric Mucosa.
Infections
The Amino Acid-mTORC1 Pathway Mediates APEC TW-XM-Induced Inflammation in bEnd.3 Cells.
Insulin Resistance
Chronic treatment with dexamethasone alters clock gene expression and melatonin synthesis in rat pineal gland at night.
Insulin Resistance
Inhibition of SNAT2 by metabolic acidosis enhances proteolysis in skeletal muscle.
Intestinal Volvulus
Characterization of the arylalkylamine N-acetyltransferase in Onchocerca volvulus.
Ischemic Attack, Transient
Arylalkylamine N-acetyltransferase (AANAT) is expressed in astrocytes and melatonin treatment maintains AANAT in the gerbil hippocampus induced by transient cerebral ischemia.
Klatskin Tumor
Overexpression of Prdx1 in hilar cholangiocarcinoma: a predictor for recurrence and prognosis.
Liver Cirrhosis
Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesis.
Liver Cirrhosis
Prolonged exposure of cholestatic rats to complete dark inhibits biliary hyperplasia and liver fibrosis.
Melanoma
Gender-specific associations between polymorphisms of the circadian gene RORA and cutaneous melanoma susceptibility.
Melanosis
A New Arylalkylamine N-Acetyltransferase in Silkworm (Bombyx mori) Affects Integument Pigmentation.
Melanosis
Mutations of an arylalkylamine-n-acetyl transferase, BM-IAANAT, are responsible for the silkworm melanism mutant.
Neoplasm Metastasis
Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target.
Neoplasms
AA-NAT, MT1 and MT2 Correlates with Cancer Stem-Like Cell Markers in Colorectal Cancer: Study of the Influence of Stage and p53 Status of Tumors.
Neoplasms
Activation of SNAT1/SLC38A1 in human breast cancer: correlation with p-Akt overexpression.
Neoplasms
Adiponectin Inhibits Nutrient Transporters and Promotes Apoptosis in Human Villous Cytotrophoblasts: Involvement in the Control of Fetal Growth.
Neoplasms
Deletion of Amino Acid Transporter ASCT2 (SLC1A5) Reveals an Essential Role for Transporters SNAT1 (SLC38A1) and SNAT2 (SLC38A2) to Sustain Glutaminolysis in Cancer Cells.
Neoplasms
Effect of TNF-alpha on the melatonin synthetic pathway in the rat pineal gland: basis for a 'feedback' of the immune response on circadian timing.
Neoplasms
Histological features and expression of enzymes implicated in melatonin synthesis in pineal parenchymal tumours and in cultured tumoural pineal cells.
Neoplasms
STAT1-NF?B crosstalk triggered by interferon gamma regulates noradrenaline-induced pineal hormonal production.
Nervous System Diseases
Selective tonicity-induced expression of the neutral amino-acid transporter SNAT2 in oligodendrocytes in rat brain following systemic hypertonicity.
Obesity
Design, synthesis and in vitro evaluation of novel benzo[b]thiophene derivatives as serotonin N-acetyltransferase (AANAT) inhibitors.
Osteosarcoma
Deletion of Amino Acid Transporter ASCT2 (SLC1A5) Reveals an Essential Role for Transporters SNAT1 (SLC38A1) and SNAT2 (SLC38A2) to Sustain Glutaminolysis in Cancer Cells.
Osteosarcoma
Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target.
Paraproteinemias
Dependence on glutamine uptake and glutamine addiction characterize myeloma cells: a new attractive target.
Peritonitis
Pineal arylalkylamine N-acetyl-transferase (Aanat) gene expression as a target of inflammatory mediators in the chicken.
Pulmonary Edema
Sodium coupled neutral amino acid transporter SNAT2 counteracts cardiogenic pulmonary edema by driving alveolar fluid clearance.
Retinoblastoma
Regulation of AA-NAT and HIOMT gene expression by butyrate and cyclic AMP in Y79 human retinoblastoma cells.
Retinoblastoma
The human serotonin N-acetyltransferase (EC 2.3.1.87) gene (AANAT): structure, chromosomal localization, and tissue expression.
Rett Syndrome
Dysregulation of Glutamine Transporter SNAT1 in Rett Syndrome Microglia: A Mechanism for Mitochondrial Dysfunction and Neurotoxicity.
Sarcopenia
Skeletal muscle: from birth to old age, routes to mechanical and metabolic failure.
Scoliosis
Association study of tryptophan hydroxylase 1 and arylalkylamine N-acetyltransferase polymorphisms with adolescent idiopathic scoliosis in Han Chinese.
Seizures
Modulation of epileptiform activity by glutamine and system A transport in a model of post-traumatic epilepsy.
Sepsis
Sepsis-induced changes in amino acid transporters and leucine signaling via mTOR in skeletal muscle.
Sleep Disorders, Circadian Rhythm
Arylalkylamine N-acetyltransferase (AANAT) genotype as a personal trait in melatonin synthesis.
Sleep Disorders, Circadian Rhythm
Significant association of the arylalkylamine N-acetyltransferase ( AA-NAT) gene with delayed sleep phase syndrome.
Sleep Disorders, Circadian Rhythm
The G619A Aa-nat gene polymorphism does not contribute to sleep time variation in the Brazilian population.
Sleep Wake Disorders
Computational Analysis of N-acetyl transferase in Tribolium castaneum.
Sleep Wake Disorders
Crystal structure of the dopamine N-acetyltransferase-acetyl-CoA complex provides insights into the catalytic mechanism.
Squamous Cell Carcinoma of Head and Neck
ASCT2 (SLC1A5)-dependent glutamine uptake is involved in the progression of head and neck squamous cell carcinoma.
Starvation
Amino acid starvation induces the SNAT2 neutral amino acid transporter by a mechanism that involves eukaryotic initiation factor 2alpha phosphorylation and cap-independent translation.
Starvation
Characterization and Regulation of the Amino Acid Transporter SNAT2 in the Small Intestine of Piglets.
Starvation
Deletion of Amino Acid Transporter ASCT2 (SLC1A5) Reveals an Essential Role for Transporters SNAT1 (SLC38A1) and SNAT2 (SLC38A2) to Sustain Glutaminolysis in Cancer Cells.
Starvation
Effects of starvation, re-feeding and timing of food supply on daily rhythm features of gut melatonin in carp (Catla catla).
Starvation
Enhanced small neutral but not branched chain amino acid transport after epigenetic sodium coupled neutral amino acid transporter-2 (SNAT2) cDNA expression in myoblasts.
Starvation
Proteasomal modulation of cellular SNAT2 (SLC38A2) abundance and function by unsaturated fatty acid availability.
Starvation
Specificity of amino acid regulated gene expression: analysis of genes subjected to either complete or single amino acid deprivation.
Starvation
The synthesis of SNAT2 transporters is required for the hypertonic stimulation of system A transport activity.
Stroke
Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system.
Uterine Cervical Neoplasms
Deletion of Amino Acid Transporter ASCT2 (SLC1A5) Reveals an Essential Role for Transporters SNAT1 (SLC38A1) and SNAT2 (SLC38A2) to Sustain Glutaminolysis in Cancer Cells.
Vitamin A Deficiency
Regulation of the expression of serotonin N-acetyltransferase gene in Japanese quail (Coturnix japonica): II. Effect of vitamin A deficiency.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0019
Arachidonoyl-CoA
pH 8.0, temperature not specified in the publication
0.0036
oleoyl-CoA
pH 8.0, temperature not specified in the publication
0.0099
palmitoyl-CoA
pH 8.0, temperature not specified in the publication
0.006
stearoyl-CoA
pH 8.0, temperature not specified in the publication
0.0061
acetyl-CoA
pH 8.0, temperature not specified in the publication
0.0018
butanoyl-CoA
pH 8.0, temperature not specified in the publication
0.11
dopamine
pH not specified in the publication, temperature not specified in the publication
0.05
norepinephrine
pH not specified in the publication, temperature not specified in the publication
0.07
octopamine
pH not specified in the publication, temperature not specified in the publication
0.1
tryptamine
pH not specified in the publication, temperature not specified in the publication
4
tryptamine
above, pH 6.8, 37°C, recombinant mutants P64A, P64G, P64W, and recombinant truncation mutants
0.56
tyramine
pH not specified in the publication, temperature not specified in the publication
additional information
additional information
formation and cleavage of a disulfide bond produce active/inactive states of enzyme
-
additional information
additional information
-
exposing chickens to light in the middle of the night increased the apparent Km of AANAT for tryptamine by about 10fold. The Km began to increase within 5 min of exposure to light and reached a maximum at about 30 min
-
additional information
additional information
-
kinetics of recombinant partially phosphorylated wild-type and mutant enzymes, overview
-
additional information
additional information
kinetics of recombinant wild-type and mutant enzymes, substrate tryptamine, overview
-
additional information
additional information
-
AANAT2 kinetic constants as a function of temperature, overview
-
additional information
additional information
-
AANAT2 kinetic constants as a function of temperature, overview
-
additional information
additional information
-
AANAT2 kinetic constants as a function of temperature, overview
-
additional information
additional information
-
AANAT2 kinetics in relation to temperature, overview
-
additional information
additional information
-
AANAT2 kinetics in relation to temperature, overview
-
additional information
additional information
-
Michaelis-Menten kinetic analysis
-
additional information
additional information
both isoforms Aanat1a and Aanat1b display 2- to 3fold lower KM values for indolethylamines compared to phenylethylamines. KM for phenylethylamines is around two times lower for Aanat1a than for Aanat1b
-
additional information
additional information
both isoforms Aanat1a and Aanat1b display 2- to 3fold lower KM values for indolethylamines compared to phenylethylamines. KM for phenylethylamines is around two times lower for Aanat1a than for Aanat1b
-
additional information
additional information
both isoforms Aanat1a and Aanat1b display 2- to 3fold lower KM values for indolethylamines compared to phenylethylamines. KM for phenylethylamines is around two times lower for Aanat1a than for Aanat1b
-
additional information
additional information
-
both isoforms Aanat1a and Aanat1b display 2- to 3fold lower KM values for indolethylamines compared to phenylethylamines. KM for phenylethylamines is around two times lower for Aanat1a than for Aanat1b
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.043
Arachidonoyl-CoA
pH 8.0, temperature not specified in the publication
0.075
oleoyl-CoA
pH 8.0, temperature not specified in the publication
0.16
palmitoyl-CoA
pH 8.0, temperature not specified in the publication
0.059
stearoyl-CoA
pH 8.0, temperature not specified in the publication
1.3
acetyl-CoA
pH 8.0, temperature not specified in the publication
0.53
butanoyl-CoA
pH 8.0, temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
23
Arachidonoyl-CoA
pH 8.0, temperature not specified in the publication
21
oleoyl-CoA
pH 8.0, temperature not specified in the publication
16
palmitoyl-CoA
pH 8.0, temperature not specified in the publication
9.8
stearoyl-CoA
pH 8.0, temperature not specified in the publication
220
acetyl-CoA
pH 8.0, temperature not specified in the publication
290
butanoyl-CoA
pH 8.0, temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00039
2-bromo-N-[2-(5-fluoro-1-benzothien-3-yl)ethyl]acetamide
Homo sapiens
-
IC50: 0.00039 mM
0.00225
methyl 3-[2-[(bromoacetyl)amino]ethyl]-1-benzothiophene-5-carboxylate
Homo sapiens
-
IC50: 0.00225 mM
0.0087
N-[2-(2-benzyl-5-methoxy-benzofuran-3-yl)ethyl]iodoacetamide
Homo sapiens
-
IC50: 0.0087 mM
0.001
N-[2-(2-phenyl-benzo [b]thiophen-3-yl)ethyl]bromoacetamide
Homo sapiens
-
IC50: 0.001 mM
0.00378
N-[2-(5-bromo-benzo[b]thiophen-3-yl)ethyl]bromoacetamide
Homo sapiens
-
IC50: 0.00378 mM
0.00018
N-[2-(5-chloro-benzo[b]thiophen-3-yl)ethyl]bromoacetamide
Homo sapiens
-
IC50: 0.00018 mM
0.00071
N-[2-(5-ethyl-benzo[b]thiophen-3-yl)ethyl]bromoacetamide
Homo sapiens
-
IC50: 0.00071 mM
0.00018
N-[2-(5-hydroxy-benzo[b]thiophen-3-yl)ethyl]bromoacetamide
Homo sapiens
-
IC50: 0.00018 mM
0.1
N-[2-(7-ethyl-1,2,3,4-tetrahydronapht-1-yl)ethyl]iodooacetamide
Homo sapiens
-
IC50: above 0.1 mM
0.045
N-[2-(7-methoxy-3-(3-trifluoromethylphenyl)-napht-1-yl)ethyl]iodoacetamide
Homo sapiens
-
IC50: 0.045 mM
0.1
N-[2-(7-methoxy-8-propenyl-napht-1-yl)ethyl]-iodoacetamide
Homo sapiens
-
IC50: above 0.1 mM
0.0008
acetyl-CoA-tryptamine
Rattus norvegicus
IC50: 0.0008 mM, GST-AANAT fusion protein
0.0051
bromoacetyltryptamine
Rattus norvegicus
IC50: 0.0051 mM, GST-AANAT fusion protein
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000027
-
enzyme activity on pineal in pineal gland from quiescent phase, pH 6.5, 25°C
0.000036
-
enzyme activity on pineal in pineal gland from progressive phase, pH 6.5, 25°C
0.000039
-
enzyme activity on pineal in pineal gland from breeding phase, pH 6.5, 25°C
0.000043
-
enzyme activity on pineal in pineal gland from regressive phase, pH 6.5, 25°C
additional information
additional information
enzyme activities of recombinant wild-type and mutant enzymes, overview
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
6.8
7.5
9.5
-
two pH optima, pH 6.0 and pH 9.5, which change during oocyte maturation
6
-
two pH optima, pH 6.0 and pH 9.5, which change during oocyte maturation