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EC Tree
IUBMB Comments The animal enzyme is a multi-functional protein catalysing the reactions of EC 2.3.1.38 [acyl-carrier-protein] S-acetyltransferase, EC 2.3.1.39 [acyl-carrier-protein] S-malonyltransferase, EC 2.3.1.41 beta-ketoacyl-[acyl-carrier-protein] synthase I, EC 1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase, EC 4.2.1.59 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, EC 1.3.1.39 enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific) and EC 3.1.2.14 oleoyl-[acyl-carrier-protein] hydrolase. cf. EC 2.3.1.86, fatty-acyl-CoA synthase system.
The taxonomic range for the selected organisms is: Bos taurus The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
fasii, fatty-acid synthase, fas-ii, type ii fatty acid synthase, yeast fatty acid synthase, fatty acid synthase ii, fatty acid synthase i, type 2 fatty acid synthase, fatty acid synthase type 2, f09e10.3 protein,
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fatty-acid synthase
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yeast fatty acid synthase
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acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
reaction mechanism
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acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
structure and regulation
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Acyl group transfer
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thioester hydrolysis
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acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing and thioester-hydrolysing)
The animal enzyme is a multi-functional protein catalysing the reactions of EC 2.3.1.38 [acyl-carrier-protein] S-acetyltransferase, EC 2.3.1.39 [acyl-carrier-protein] S-malonyltransferase, EC 2.3.1.41 beta-ketoacyl-[acyl-carrier-protein] synthase I, EC 1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase, EC 4.2.1.59 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, EC 1.3.1.39 enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific) and EC 3.1.2.14 oleoyl-[acyl-carrier-protein] hydrolase. cf. EC 2.3.1.86, fatty-acyl-CoA synthase system.
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acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
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acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
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acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
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C20 and C22 fatty acids in the absence of thioesterase activity
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acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
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multifunctional enzyme, involved in animal fat synthesis
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acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
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multifunctional enzyme, involved in animal fat synthesis
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4'-phosphopantetheine
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requirement, 1 mol associated with 1 mol subunit
additional information
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no requirement for FMN
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NADPH
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NADPH
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requirement, high specificity
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1,3-Dibromo-2-propanone
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diisopropylfluorophosphate
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iodoacetamide
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beta-ketoacyl synthetase activity, acetyl-CoA but not malonyl-CoA protects
pyridoxal 5'-phosphate
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enoyl reductase activity, NADPH protects
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UniProt
brenda
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brenda
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brenda
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physiological function
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fatty acid synthase is a key enzyme regulating de novo fatty acid synthesis
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FAS_BOVIN
2513
0
274554
Swiss-Prot
other Location (Reliability: 2 )
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additional information
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dissociation of native enzyme leads to loss of activity
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0°C, inactivation after 12 h, reactivation after 2 h at 25°C in the presence of NADPH, not acetyl-CoA or NADH
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relative FASN mRNA expression is upregulated by 9 and 5fold, respectively, for high-concentrate diet (85% concentrate: 15% roughage) and diet on endophyte-free tall fescue pastures with corn grain supplement compared with pasture diet, grain feeding upregulates FASN mRNA expression
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analysis
simple mass spectrometry-based assay that affords monitoring of FASN activity and its product specificity. Purified FASN is incubated with 13C-labeled malonyl-CoA, acetyl-CoA, and NADPH, at defined time points the reaction mixture is spiked with an internal non-esterified palmitic acid standard and extracted, and the extract is analyzed directly, without vacuum evaporation and chemical derivatization, by direct-infusion high-resolution mass spectrometry in negative ion mode. The assay supports essentially noise-free detection and absolute quantification of de novo synthetized 13C-labeled non-esterified fatty acids
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Wakil, S.J.; Stoops, J.K.; Joshi, V.C.
Fatty acid synthesis and its regulation
Annu. Rev. Biochem.
52
537-579
1983
Anser sp., Bos taurus, Canis lupus familiaris, Gallus gallus, Columba sp., Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus
brenda
Duckett, S.K.; Pratt, S.L.; Pavan, E.
Corn oil or corn grain supplementation to steers grazing endophyte-free tall fescue. II. Effects on subcutaneous fatty acid content and lipogenic gene expression
J. Anim. Sci.
87
1120-1128
2009
Bos taurus
brenda
Topolska, M.; Martinez-Montanes, F.; Ejsing, C.S.
Topolska, M.; Martixadnez-Montanes, F.; Ejsing, C. A simple and direct assay for monitoring fatty acid synthase activity and product-specificity by high-resolution mass spectrometry
Biomolecules
10
118
2020
Bos taurus (Q71SP7)
brenda