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4-bromo-thiophene-2-carbonyl-S-CoA + amikacin
?
-
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + gentamicin
?
-
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
?
-
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + paromomycin
?
-
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + tobramycin
?
-
-
-
-
?
5-bromo-thiophene-2-carbonyl-S-CoA + kanamycin A
?
-
-
-
-
?
5-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
?
-
-
-
-
?
6-fluoropicolinyl-CoA + neomycin B
?
-
-
-
-
?
acetyl-CoA + a 2-deoxystreptamine antibiotic
CoA + N3-acetyl-2-deoxystreptamine antibiotic
acetyl-CoA + amikacin
CoA + N3'-acetyl-amikacin
-
-
-
-
?
acetyl-CoA + amikacin
CoA + N3'-acetylamikacin
acetyl-CoA + aminoglycoside-aminocyclitol
CoA + ?
-
-
-
?
acetyl-CoA + apramycin
CoA + N3'-acetylapramycin
acetyl-CoA + butirosin
CoA + N3'-acetylbutirosin
-
-
-
?
acetyl-CoA + dactimicin
CoA + N4-acetyldactamicin + N4,N6'-diacetyldactimicin
-
the percentage of dactimicin affinity relative to gentamicin C1a is very high (74.6%) only with AAC(3)-I, to the most other enzymes tested, in spite of the bifunctional AAC(6')/APH(2''), dactamicin is stable
-
-
?
acetyl-CoA + dibekacin
CoA + N3''-acetyldibekacin
acetyl-CoA + dibekacin
CoA + N3-acetyldibekacin
acetyl-CoA + fortimicin
CoA + N3-acetylfortimicin
-
-
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetyl-gentamicin
-
-
-
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetylgentamicin
acetyl-CoA + gentamicin A
CoA + N3'-acetylgentamicin A
-
acetylation at 6% the rate of gentamicin C1
-
-
?
acetyl-CoA + gentamicin C
CoA + N3'-acetylgentamicin C
-
acetylation at 86% the rate of gentamicin C1
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
acetyl-CoA + gentamicin C1A
CoA + N3-acetylgentamicin C1A
-
-
-
?
acetyl-CoA + gentamicin C2
CoA + N3'-acetylgentamicin C2
-
acetylation at 84% the rate of gentamicin C1
-
-
?
acetyl-CoA + isepamycin
CoA + N3'-acetylisepamycin
wild-type and recombinant enzyme
-
?
acetyl-CoA + kanamycin
CoA + N3'-acetyl kanamycin
-
-
-
?
acetyl-CoA + kanamycin
CoA + N3'-acetylkanamycin
acetyl-CoA + kanamycin A
CoA + N3'-acetyl-kanamycin A
-
-
-
-
?
acetyl-CoA + kanamycin A
CoA + N3'-acetylkanamycin A
acetyl-CoA + kanamycin B
CoA + N3'-acetylkanamycin B
acetyl-CoA + kanamycin B
CoA + N3-acetylkanamycin B
acetyl-CoA + lividomycin A
CoA + ?
-
-
-
-
?
acetyl-CoA + neomycin
CoA + ?
-
-
-
?
acetyl-CoA + neomycin
CoA + N2'-acetylneomycin
wild-type and recombinant enzyme
-
?
acetyl-CoA + neomycin
CoA + N3-acetylneomycin
-
-
-
?
acetyl-CoA + neomycin B
CoA + ?
acetyl-CoA + neomycin B
CoA + N3-acetyl-neomycin B
acetyl-CoA + neomycin B
CoA + N3-acetylneomycin B
-
-
-
-
?
acetyl-CoA + netilmicin
CoA + N3'-acetylnetilmicin
acetyl-CoA + paromomycin
CoA + N3'-acetyl-paromomycin
acetyl-CoA + paromomycin
CoA + N3'-acetylparomomycin
-
-
-
?
acetyl-CoA + paromomycin
CoA + N3'-acetylparomomycin B
-
not E. coli
-
-
?
acetyl-CoA + paromomycin
CoA + N3-acetylparamomycin
-
-
-
?
acetyl-CoA + ribostamycin
CoA + ?
acetyl-CoA + ribostamycin
CoA + N3-acetylribostamycin
-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetyl-sisomicin
-
-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
acetyl-CoA + sisomicin
CoA + N3-acetylsisomicin
-
-
-
?
acetyl-CoA + sisomycin
CoA + N3'-acetylsisomycin
acetyl-CoA + spectinomycin
CoA + N3'-acetylspectinomycin
wild-type and recombinant enzyme
-
?
acetyl-CoA + streptomycin
CoA + ?
wild-type and recombinant enzyme
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyl-tobramycin
-
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
acetyl-CoA + tobramycin
CoA + N3-acetyltobramycin
-
-
-
?
butyryl-CoA + kanamycin A
CoA + N3'-butyryl-kanamycin A
-
-
-
-
?
butyryl-CoA + ribostamycin
CoA + ?
-
-
-
?
desulfo-CoA + ribostamycin
?
-
-
-
?
glutaryl-CoA + kanamycin A
?
-
-
-
-
?
glycyl-CoA + amikacin
CoA + N3'-glycyl-amikacin
-
-
-
-
?
glycyl-CoA + gentamicin
CoA + N3'-glycyl-gentamicin
-
-
-
-
?
glycyl-CoA + kanamycin A
CoA + N3'-glycyl-kanamycin A
-
-
-
-
?
glycyl-CoA + neomycin B
CoA + N3-glycylneomycin B
-
-
-
-
?
glycyl-CoA + sisomicin
CoA + N3'-glycyl-sisomicin
-
-
-
-
?
malonyl-CoA + amikacin
CoA + N3'-malonyl-amikacin
-
-
-
-
?
malonyl-CoA + gentamicin
CoA + N3'-malonyl-gentamicin
-
-
-
-
?
malonyl-CoA + kanamycin A
CoA + N3'-malonylkanamycin A
-
-
-
-
?
malonyl-CoA + neomycin B
CoA + N3-malonylneomycin B
-
-
-
-
?
malonyl-CoA + ribostamycin
CoA + ?
-
-
-
?
methylmalonyl-CoA + neomycin B
CoA + N3-methylmalonylneomycin B
-
-
-
-
?
N-propionyl-CoA + amikacin
CoA + N3'-propionyl-amikacin
-
-
-
-
?
N-propionyl-CoA + gentamicin
CoA + N3'-propionyl-gentamicin
-
-
-
-
?
n-propionyl-CoA + kanamycin A
CoA + N3'-n-propionylkanamycin A
-
-
-
-
?
N-propionyl-CoA + kanamycin A
CoA + N3'-propionyl-kanamycin A
-
-
-
-
?
N-propionyl-CoA + neomycin B
CoA + N3-propionylneomycin B
-
-
-
-
?
N-propionyl-CoA + paromomycin
CoA + N3'-propionyl-paromomycin
-
-
-
-
?
N-propionyl-CoA + sisomicin
CoA + N3'-propionyl-sisomicin
-
-
-
-
?
N-propionyl-CoA + tobramycin
CoA + N3'-propionyl-tobramycin
-
-
-
-
?
propionyl-CoA + ribostamycin
CoA + ?
-
-
-
?
additional information
?
-
acetyl-CoA + a 2-deoxystreptamine antibiotic

CoA + N3-acetyl-2-deoxystreptamine antibiotic
-
-
-
?
acetyl-CoA + a 2-deoxystreptamine antibiotic
CoA + N3-acetyl-2-deoxystreptamine antibiotic
-
-
-
?
acetyl-CoA + amikacin

CoA + N3'-acetylamikacin
-
-
-
?
acetyl-CoA + amikacin
CoA + N3'-acetylamikacin
-
-
-
?
acetyl-CoA + amikacin
CoA + N3'-acetylamikacin
wild-type and recombinant enzyme
-
?
acetyl-CoA + apramycin

CoA + N3'-acetylapramycin
Arizona sp.
-
a wide range of antibiotics containing the 2-deoxystreptamine ring can act as acceptors, including gentamicin, kanamycin, tobramycin, neomycin
-
?
acetyl-CoA + apramycin
CoA + N3'-acetylapramycin
-
-
-
?
acetyl-CoA + apramycin
CoA + N3'-acetylapramycin
-
a wide range of antibiotics containing the 2-deoxystreptamine ring can act as acceptors, including gentamicin, kanamycin, tobramycin, neomycin
-
?
acetyl-CoA + apramycin
CoA + N3'-acetylapramycin
-
-
-
?
acetyl-CoA + apramycin
CoA + N3'-acetylapramycin
-
a wide range of antibiotics containing the 2-deoxystreptamine ring can act as acceptors, including gentamicin, kanamycin, tobramycin, neomycin
-
?
acetyl-CoA + dibekacin

CoA + N3''-acetyldibekacin
-
-
-
?
acetyl-CoA + dibekacin
CoA + N3''-acetyldibekacin
-
-
-
?
acetyl-CoA + dibekacin
CoA + N3''-acetyldibekacin
-
-
-
-
?
acetyl-CoA + dibekacin
CoA + N3''-acetyldibekacin
-
-
-
-
?
acetyl-CoA + dibekacin
CoA + N3''-acetyldibekacin
-
acetylation at 8% the rate of gentamicin C1
-
-
?
acetyl-CoA + dibekacin

CoA + N3-acetyldibekacin
-
product identification by high-field NMR
-
?
acetyl-CoA + dibekacin
CoA + N3-acetyldibekacin
-
product identification by high-field NMR
-
?
acetyl-CoA + gentamicin

CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetylgentamicin
wild-type and recombinant enzyme
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + gentamicin C1

CoA + N3'-acetylgentamicin C1
-
-
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
-
-
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
-
-
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
-
best substrate
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
-
-
-
-
?
acetyl-CoA + gentamicin C1a

CoA + N3'-acetylgentamicin C1a
-
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
-
-
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
-
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
-
-
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
-
acetylation at 60% the rate of gentamicin C1
-
?
acetyl-CoA + kanamycin

CoA + N3'-acetylkanamycin
-
-
-
?
acetyl-CoA + kanamycin
CoA + N3'-acetylkanamycin
-
-
-
?
acetyl-CoA + kanamycin
CoA + N3'-acetylkanamycin
wild-type and recombinant enzyme
-
-
acetyl-CoA + kanamycin A

CoA + N3'-acetylkanamycin A
-
-
-
-
?
acetyl-CoA + kanamycin A
CoA + N3'-acetylkanamycin A
-
-
-
-
?
acetyl-CoA + kanamycin A
CoA + N3'-acetylkanamycin A
-
-
-
-
?
acetyl-CoA + kanamycin A
CoA + N3'-acetylkanamycin A
-
-
-
?
acetyl-CoA + kanamycin A
CoA + N3'-acetylkanamycin A
-
acetylation at 7% the rate of gentamicin C1
-
-
?
acetyl-CoA + kanamycin A
CoA + N3'-acetylkanamycin A
-
-
-
-
?
acetyl-CoA + kanamycin B

CoA + N3'-acetylkanamycin B
-
-
-
-
acetyl-CoA + kanamycin B
CoA + N3'-acetylkanamycin B
-
-
-
-
acetyl-CoA + kanamycin B
CoA + N3'-acetylkanamycin B
-
-
-
-
?
acetyl-CoA + kanamycin B
CoA + N3'-acetylkanamycin B
-
-
-
?
acetyl-CoA + kanamycin B

CoA + N3-acetylkanamycin B
-
-
-
?
acetyl-CoA + kanamycin B
CoA + N3-acetylkanamycin B
-
-
-
?
acetyl-CoA + neomycin B

CoA + ?
-
-
-
-
?
acetyl-CoA + neomycin B
CoA + ?
-
-
-
-
?
acetyl-CoA + neomycin B

CoA + N3-acetyl-neomycin B
-
-
-
-
?
acetyl-CoA + neomycin B
CoA + N3-acetyl-neomycin B
-
-
-
-
?
acetyl-CoA + netilmicin

CoA + N3'-acetylnetilmicin
-
-
-
?
acetyl-CoA + netilmicin
CoA + N3'-acetylnetilmicin
-
the much higher treshold value for the modification of netilmicin as compared with the one for the modification of sisomicin is due to the 1-N side chain
-
-
?
acetyl-CoA + netilmicin
CoA + N3'-acetylnetilmicin
-
the much higher treshold value for the modification of netilmicin as compared with the one for the modification of sisomicin is due to the 1-N side chain
-
-
?
acetyl-CoA + netilmicin
CoA + N3'-acetylnetilmicin
wild-type and recombinant enzyme
-
?
acetyl-CoA + paromomycin

CoA + N3'-acetyl-paromomycin
-
-
-
-
?
acetyl-CoA + paromomycin
CoA + N3'-acetyl-paromomycin
-
-
-
-
?
acetyl-CoA + ribostamycin

CoA + ?
-
-
-
?
acetyl-CoA + ribostamycin
CoA + ?
-
-
-
-
?
acetyl-CoA + sisomicin

CoA + N3'-acetylsisomicin
-
-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
best substrate
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
best substrate
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
best substrate
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
best substrate
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
-
-
-
-
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
best substrate
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
wild-type and recombinant enzyme
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
acetylation at 51% the rate of gentamicin C1
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
-
best substrate
-
?
acetyl-CoA + sisomycin

CoA + N3'-acetylsisomycin
-
-
-
?
acetyl-CoA + sisomycin
CoA + N3'-acetylsisomycin
-
-
-
-
?
acetyl-CoA + sisomycin
CoA + N3'-acetylsisomycin
-
-
-
?
acetyl-CoA + tobramycin

CoA + N3'-acetyltobramycin
-
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
wild-type and recombinant enzyme
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
acetylation at 6% the rate of gentamicin C1
-
-
?
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
-
-
-
-
?
additional information

?
-
determination of imipenem, meropenem, and gentamicin resistant clinical isolates
-
-
-
additional information
?
-
the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin
-
-
-
additional information
?
-
-
the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin
-
-
-
additional information
?
-
the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin
-
-
-
additional information
?
-
-
the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin
-
-
-
additional information
?
-
-
no substrates are amikacin
-
-
-
additional information
?
-
-
no activity with benzoyl-CoA, acetoacetyl-CoA, crotonyl-CoA, isovaleryl-CoA, palmitoyl-CoA, and DL-3-hydroxy-butyryl-CoA by AAC(6')-APH(2'')
-
-
-
additional information
?
-
-
no substrates are amikacin
-
-
-
additional information
?
-
-
no substrates are amikacin
-
-
-
additional information
?
-
-
no substrates are amikacin
-
-
-
additional information
?
-
-
lividomycin A, butirosin A and B
-
-
-
additional information
?
-
-
CoASH associates with a high-affinity, catalytic site and with a secondary, low-affinity site that overlaps with the antibiotic binding pocket. The binding of CoASH to the high-affinity site occurs with a small, unfavorable enthalpy and a favorable entropy. Binding to the second site is highly exothermic and is accompanied by an unfavorable entropic contribution. The presence of an aminoglycoside alters the binding of CoASH to the enzyme dramatically such that the binding occurs with a favorable enthalpy and an unfavorable entropy. This is irrespective of which aminoglycoside is the cosubstrate and occurs without a significant change in the affinity of CoASH for the enzyme. The presence of antibiotics eliminates binding of CoASH to the second site
-
-
-
additional information
?
-
-
enzyme displays molecular size-dependent stoichiometry where binding stoichiometries are 1.5-2.0 for small antibiotics and 1.0 for larger. Antibiotic-enzyme interaction occurs with a favorable enthalpy and a compensating unfavorable entropy. The presence of coenzyme A significantly increases the affinity of the antibioticthe enthalpy of binding becomes more favored by 1.7-10.0fold in the presence of the cosubstrate CoASH, while the entropy becomes 2.0-22.5fold less favored. The overall free energy change is still only 1.0-1.9 kcal/mol from binary to ternary for all antibiotics tested, similar to those for other aminoglycoside-modifying enzymes
-
-
-
additional information
?
-
isoform AAC-IIIb demonstrates fast antibiotic turnover rates, high antibiotic affinity. Aminoglycoside binding becomes more enthalpically favored and entropically disfavored when CoASH is complexed to AAC-IIIb. The enzyme catalytically and enthalpically favor those aminoglycosides with amine groups at the 2' carbon and show an increase in affinity of all aminoglycosides when CoASH is present
-
-
-
additional information
?
-
-
no substrates are amikacin
-
-
-
additional information
?
-
-
AAC(3)-Id is responsible for the gain of resistance to diverse agents, i.e. gentamicin C1a, gentamicin C1, sisomicin, neomycin, dibekacin, kanamycin, tobramycin, amikacin, netilmicin, apramycin, dactimicin, spectinomycin, streptomycin, lividomycin, and butirosin, in Salmonella enterica serovar Haifa, overview
-
-
-
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0.00067
apramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.237
butirosin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
1.16
butyryl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0092
dibekacin
pH 6.0, 37°C; recombinant His-tagged enzyme, pH 6.0, 37°C
0.0029 - 0.0189
gentamicin
0.00012
gentamicin C1a
-
-
0.00097
gentamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.002 - 0.238
kanamycin A
0.0026 - 0.0138
kanamycin B
0.0036
lividomycin A
-
pH 7.6, 25°C
0.234 - 0.477
malonyl-CoA
0.0886
n-propionyl-CoA
-
cosubstrate kanamycin A, pH 7.6, 25°C
0.00036
neomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0012 - 0.0148
neomycin B
0.042
netilmicin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.00015 - 0.0033
paromomycin
0.499
propionyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0023 - 0.0031
ribostamycin
0.00057 - 0.0015
sisomicin
0.00033 - 0.0015
sisomycin
0.0013 - 0.0054
tobramycin
additional information
additional information
-
0.0013
acetyl-CoA

-
cosubstrate gentamicin C1a
0.0017
acetyl-CoA
-
cosubstrate tobramycin
0.0027
acetyl-CoA
pH 6.0, 37°C; recombinant His-tagged enzyme, pH 6.0, 37°C
0.0154
acetyl-CoA
-
cosubstrate kanamycin A, pH 7.6, 25°C
0.086
acetyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.17
acetyl-CoA
-
cosubstrate neomycin B, pH 7.6, 25°C
0.00096
amikacin

-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0021
amikacin
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0716
amikacin
-
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
11.07
amikacin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0029
gentamicin

-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0046
gentamicin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0189
gentamicin
-
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
0.002
kanamycin A

-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
0.0033
kanamycin A
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
0.0048
kanamycin A
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
0.0048
kanamycin A
-
pH 7.6, 25°C
0.0048
kanamycin A
pH 7.6, 25°C
0.0055
kanamycin A
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0091
kanamycin A
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
0.0099
kanamycin A
-
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
0.0111
kanamycin A
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0135
kanamycin A
-
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
0.238
kanamycin A
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0026
kanamycin B

-
pH 7.6, 25°C
0.0026
kanamycin B
pH 7.6, 25°C
0.0138
kanamycin B
pH 6.0, 37°C; recombinant His-tagged enzyme, pH 6.0, 37°C
0.234
malonyl-CoA

-
cosubstrate kanamycin A, pH 7.6, 25°C
0.477
malonyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0012
neomycin B

-
pH 7.6, 25°C
0.0031
neomycin B
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0148
neomycin B
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.00015
paromomycin

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0033
paromomycin
-
pH 7.6, 25°C
0.0023
ribostamycin

-
pH 7.6, 25°C
0.0031
ribostamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.00057
sisomicin

-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.000724
sisomicin
-
pH 7.2, 34°C
0.0015
sisomicin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.00033
sisomycin

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0015
sisomycin
-
pH 7.6, 25°C
0.0015
sisomycin
pH 7.6, 25°C
0.0013
tobramycin

-
pH 7.6, 25°C
0.0013
tobramycin
pH 7.6, 25°C
0.00142
tobramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.0027
tobramycin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.0054
tobramycin
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
additional information
additional information

-
kinetic pattern
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
61
apramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
3.5
butirosin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.3
butyryl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.17
dibekacin
pH 6.0, 37°C; recombinant His-tagged enzyme, pH 6.0, 37°C
40
gentamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
33.8
lividomycin A
-
pH 7.6, 25°C
10.2
n-propionyl-CoA
-
cosubstrate kanamycin A, pH 7.6, 25°C
34
neomycin E
pH 7.6, 25°C
32
netilmicin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
33
propionyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.24
acetyl-CoA

pH 6.0, 37°C; recombinant His-tagged enzyme, pH 6.0, 37°C
10.5
acetyl-CoA
-
cosubstrate neomycin B, pH 7.6, 25°C
39
acetyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
39.4
acetyl-CoA
-
cosubstrate kanamycin A, pH 7.6, 25°C
0.124
amikacin

-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.126
amikacin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.146
amikacin
-
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
0.5
amikacin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.041
gentamicin

-
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
0.123
gentamicin
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.182
gentamicin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.012
kanamycin A

-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
0.017
kanamycin A
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
0.044
kanamycin A
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
0.134
kanamycin A
-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.144
kanamycin A
-
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
0.163
kanamycin A
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
0.3
kanamycin A
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
0.559
kanamycin A
-
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
51
kanamycin A
pH 7.6, 25°C
51.4
kanamycin A
-
pH 7.6, 25°C
80
kanamycin A
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.23
kanamycin B

pH 6.0, 37°C; recombinant His-tagged enzyme, pH 6.0, 37°C
54.5
kanamycin B
-
pH 7.6, 25°C
55
kanamycin B
pH 7.6, 25°C
5.7
malonyl-CoA

-
cosubstrate kanamycin A, pH 7.6, 25°C
9
malonyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
10
neomycin

pH 7.6, 25°C
60
neomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.076
neomycin B

-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.188
neomycin B
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
9.5
neomycin B
-
pH 7.6, 25°C
15
paromomycin

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
33.6
paromomycin
-
pH 7.6, 25°C
23.5
ribostamycin

-
pH 7.6, 25°C
24
ribostamycin
pH 7.6, 25°C
146
ribostamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.121
sisomicin

-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.173
sisomicin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
46
sisomycin

pH 7.6, 25°C
46.3
sisomycin
-
pH 7.6, 25°C
51
sisomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
0.089
tobramycin

-
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
0.179
tobramycin
-
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
47.8
tobramycin
-
pH 7.6, 25°C
48
tobramycin
pH 7.6, 25°C
162
tobramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
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49
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