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Information on EC 2.3.1.80 - cysteine-S-conjugate N-acetyltransferase and Organism(s) Homo sapiens and UniProt Accession Q9UHE5

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IUBMB Comments
S-Benzyl-L-cysteine and, in decreasing order of activity, S-butyl-L-cysteine, S-propyl-L-cysteine, O-benzyl-L-serine and S-ethyl-L-cysteine, can act as acceptors.
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This record set is specific for:
Homo sapiens
UNIPROT: Q9UHE5
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
cysteine-s-conjugate n-acetyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetyltransferase, cysteine S-conjugate N-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:S-substituted L-cysteine N-acetyltransferase
S-Benzyl-L-cysteine and, in decreasing order of activity, S-butyl-L-cysteine, S-propyl-L-cysteine, O-benzyl-L-serine and S-ethyl-L-cysteine, can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
81725-80-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + leukotriene E4
CoA + N-acetyl-leukotriene E4
show the reaction diagram
about 1% of the activity with S-benzyl-L-cysteine
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-
?
acetyl-CoA + S-1-menaphthyl-L-cysteine
CoA + N-acetyl-S-1-menaphthyl-L-cysteine
show the reaction diagram
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-
-
?
acetyl-CoA + S-4-nitrobenzyl-L-cysteine
CoA + N-acetyl-S-4-nitrobenzyl-L-cysteine
show the reaction diagram
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-
-
?
acetyl-CoA + S-benzyl-L-cysteine
CoA + N-acetyl-S-benzyl-L-cysteine
show the reaction diagram
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-
?
S-(1,2,3,4,4-pentachloro-butadienyl)-L-cysteine + acetyl-CoA
N-acetyl-S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine + CoA
show the reaction diagram
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?
additional information
?
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besides S-benzyl-L-cysteine and leukotriene E4, enzyme is inactive on physiological amines or amino acids L-aspartate, L-lysine, L-cystine, L-methionine, L-ethionine, L-cystathionine, or L-phenylalanine, L-tyrosine, and L-tryptophan
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?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis-2-nitrobenzoic acid
strong inhibition
octyl glucoside
10 mM, 80% residual activity. Slight activation at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
octyl glucoside
1 mM, slight stimulation, inhibitory at higher concentration
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
acetyl-CoA
pH 7.5, 37°C
0.0267
S-1-menaphthyl-L-cysteine
pH 7.5, 37°C
0.0291
S-4-nitrobenzyl-L-cysteine
pH 7.5, 37°C
0.0285 - 0.064
S-Benzyl-L-cysteine
0.19
S-(1,2,3,4,4-pentachloro-butadienyl)-L-cysteine
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NAT8_HUMAN
227
1
25619
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
x * 29000, SDS-PAGE of recombinant protein including His-tag
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 29000, SDS-PAGE of recombinant protein including His-tag
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E104K
frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold
F143S
frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold
R149K
suppression of activity
additional information
a truncated protein starting from residue M25 shows no cysteinyl-S-conjugate N-acetyltransferase activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in HEK-293T cell
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
the frequently found single nucleotide polymorphisms, E104K or F143S, do not change enzymic activity or the expression of protein by more than 2fold
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Green, T.; Lee, R.; Farrar, D.; Hill, J.
Assessing the health risks following environmental exposure to hexachlorobutadiene
Toxicol. Lett.
138
63-73
2003
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Veiga-da-Cunha, M.; Tyteca, D.; Stroobant, V.; Courtoy, P.J.; Opperdoes, F.R.; Van Schaftingen, E.
Molecular identification of NAT8 as the enzyme that acetylates cysteine S-conjugates to mercapturic acids
J. Biol. Chem.
285
18888-18898
2010
Homo sapiens (Q9UHE5)
Manually annotated by BRENDA team
Deol, R.; Josephy, P.
Acetylation of aromatic cysteine conjugates by recombinant human N-acetyltransferase 8
Xenobiotica
47
202-207
2017
Homo sapiens (Q9UHE5)
Manually annotated by BRENDA team