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Information on EC 2.3.1.74 - chalcone synthase and Organism(s) Arabidopsis thaliana and UniProt Accession P13114

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.74 chalcone synthase
IUBMB Comments
The enzyme catalyses the first committed step in the biosynthesis of flavonoids. It can also act on dihydro-4-coumaroyl-CoA, forming phloretin.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: P13114
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
chalcone synthase, ascl1, ascl2, chs-1, chs_h1, gmirchs, flavanone synthase, ripks4, chs-7, 6'-deoxychalcone synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6'-deoxychalcone synthase
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chalcone synthase
chalcone synthetase
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-
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DOCS
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-
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flavanone synthase
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flavanone synthetase
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naringenin-chalcone synthase
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synthase, flavanone
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vsad1
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gene name, verticillium sensitive and anthocyanin deficient 1
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:4-coumaroyl-CoA malonyltransferase (cyclizing)
The enzyme catalyses the first committed step in the biosynthesis of flavonoids. It can also act on dihydro-4-coumaroyl-CoA, forming phloretin.
CAS REGISTRY NUMBER
COMMENTARY hide
56803-04-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3 malonyl-CoA + 4-coumaroyl-CoA
4 CoA + naringenin chalcone + 3 CO2
show the reaction diagram
3 malonyl-CoA + 4-coumaroyl-CoA
4 CoA + naringenin chalcone + 3 CO2
show the reaction diagram
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-
-
-
?
4-coumaroyl-CoA + 3 malonyl-CoA
naringenin chalcone + 4 CoA + 3 CO2
show the reaction diagram
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-
-
-
?
malonyl-CoA + 4-coumaroyl-CoA
naringenin chalcone + CoA + CO2
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3 malonyl-CoA + 4-coumaroyl-CoA
4 CoA + naringenin chalcone + 3 CO2
show the reaction diagram
3 malonyl-CoA + 4-coumaroyl-CoA
4 CoA + naringenin chalcone + 3 CO2
show the reaction diagram
-
-
-
-
?
4-coumaroyl-CoA + 3 malonyl-CoA
naringenin chalcone + 4 CoA + 3 CO2
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHSY_ARATH
395
0
43116
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
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calculated from amino acid sequence
69000
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fusion protein with glutathione S-transferase, SDS-PAGE
additional information
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comparison of amino acid sequence with resveratrol synthase, EC 2.3.1.95
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ubiquitination
Kelch domain-containing F-box protein physically interacts with the enzyme and specifically mediates its ubiquitination and degradation
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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construction of transgenic Arabidosis thaliana plants expressing four different viral suppressor proteins, i.e. the p38 protein of Turnip Crinkle Virus, the p25 protein of Potato Virus X, the 2b proteins of Cucumber Mosaic Virus and Tomato Aspermy Virus, using transfection with Agrobacterium tumefaciens, in the plants purple anthocyanins pigments accumulate to very high levels in the rosette leaves and stem during plant development, phenotypes, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Red anti-HA affinity gel filtration
near homogeneity
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) pLysS cells
expressed in Nicotiana tabacum leaves
fusion protein with glutathione S-transferase
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gene CHS, realtime PCR expression analysis
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
overexpression of the nuclear-localized protein MOS9 results in a reduction of enzyme transcript levels
ability and effectiveness of four different viral suppressor proteins, i.e. the p38 protein of Turnip Crinkle Virus, the p25 protein of Potato Virus X, the 2b proteins of Cucumber Mosaic Virus and Tomato Aspermy Virus, that interfere with posttranscriptional gene silencing of the endogenous chalcone synthase gene in Arabidopsis thaliana when the silencing trigger and the viral suppressor protein are expressed from the same transgene locus. The silencing trigger consists of an inverted-repeat transgene construct that induces PTGS of the endogenous Arabidopsis thaliana CHS gene with high efficiency, overview
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enzyme expression is induced in response to Verticillium dahliae infection
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cain, C.C.; Saslowsky, D.E.; Walker, R.A.; Shirley, B.W.
Expression of chalcone synthase and chalcone isomerase proteins in Arabidopsis seedlings
Plant Mol. Biol.
35
377-381
1997
Arabidopsis thaliana
Manually annotated by BRENDA team
Schrder, J.; Schrder, G.
Stilbene and chalcone synthases: Related enzymes with key functions in plant-specific pathways
Z. Naturforsch. C
45c
1-8
1990
Arabidopsis thaliana, Glycine max, Sinapis alba
-
Manually annotated by BRENDA team
Dana, C.D.; Bevan, D.R.; Winkel, B.S.
Molecular modeling of the effects of mutant alleles on chalcone synthase protein structure
J. Mol. Model.
12
905-914
2006
Arabidopsis thaliana (P13114)
Manually annotated by BRENDA team
Johansen, W.; Wilson, R.C.
Viral suppressor proteins show varying abilities and effectiveness to suppress transgene-induced post-transcriptional gene silencing of endogenous chalcone synthase in transgenic Arabidopsis
Plant Cell Rep.
27
911-921
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Lei, K.; Zhang, L.; Du, X.; An, Y.; Chang, G.; An, G.
A chalcone synthase controls the verticillium disease resistance response in both Arabidopsis thaliana and cotton
Eur. J. Plant Pathol.
152
769-781
2018
Arabidopsis thaliana, Gossypium hirsutum
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Manually annotated by BRENDA team
Watkinson, J.I.; Bowerman, P.A.; Crosby, K.C.; Hildreth, S.B.; Helm, R.F.; Winkel, B.S.J.
Identification of MOS9 as an interaction partner for chalcone synthase in the nucleus
PeerJ
6
e5598
2018
Arabidopsis thaliana (P13114)
Manually annotated by BRENDA team
Zhang, X.; Abrahan, C.; Colquhoun, T.A.; Liu, C.J.
A proteolytic regulator controlling chalcone synthase stability and flavonoid biosynthesis in Arabidopsis
Plant Cell
29
1157-1174
2017
Arabidopsis thaliana (P13114), Arabidopsis thaliana
Manually annotated by BRENDA team