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Information on EC 2.3.1.71 - glycine N-benzoyltransferase and Organism(s) Mus musculus and UniProt Accession Q91XE0

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EC Tree
IUBMB Comments
Not identical with EC 2.3.1.13, glycine N-acyltransferase or EC 2.3.1.68, glutamine N-acyltransferase
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Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: Q91XE0
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
benzoyl-coa:glycine n-acyltransferase, aralkyl-coa:glycine n-acyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aralkyl-CoA:glycine N-acyltransferase
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benzoyl CoA-amino acid N-acyltransferase
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benzoyl-CoA:glycine N-acyltransferase
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benzoyltransferase, glycine
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glycine N-acyltransferase
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GNAT
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
benzoyl-CoA:glycine N-benzoyltransferase
Not identical with EC 2.3.1.13, glycine N-acyltransferase or EC 2.3.1.68, glutamine N-acyltransferase
CAS REGISTRY NUMBER
COMMENTARY hide
71567-07-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzoyl-CoA + glycine
CoA + N-benzoylglycine
show the reaction diagram
glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate
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-
?
benzoyl-CoA + glycine ethyl ester
CoA + N-benzoylglycine ethyl ester
show the reaction diagram
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-
-
?
benzoyl-CoA + glycine methyl ester
CoA + N-benzoylglycine methyl ester
show the reaction diagram
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-
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?
additional information
?
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enzyme also shows glycine N-acyltransferase activity, reaction of EC 2.3.1.13. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycolic acid
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oleoyl-CoA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0094
benzoyl-CoA
pH 8.0, temperature not specified in the publication
6.1
glycine
pH 8.0, temperature not specified in the publication
9.7
glycine ethyl ester
pH 8.0, temperature not specified in the publication
29
Glycine methyl ester
pH 8.0, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.2
benzoyl-CoA
pH 8.0, temperature not specified in the publication
3.2
glycine
pH 8.0, temperature not specified in the publication
0.57
glycine ethyl ester
pH 8.0, temperature not specified in the publication
1
Glycine methyl ester
pH 8.0, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
450
benzoyl-CoA
pH 8.0, temperature not specified in the publication
0.52
glycine
pH 8.0, temperature not specified in the publication
0.059
glycine ethyl ester
pH 8.0, temperature not specified in the publication
0.035
Glycine methyl ester
pH 8.0, temperature not specified in the publication
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
58
glycolic acid
Mus musculus
pH 8.0, temperature not specified in the publication
0.021
oleoyl-CoA
Mus musculus
pH 8.0, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GLYAT_MOUSE
296
0
34098
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
x * 34000, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 34000, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dempsey, D.R.; Bond, J.D.; Carpenter, A.M.; Rodriguez Ospina, S.; Merkler, D.J.
Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli
Protein Expr. Purif.
97
23-28
2014
Mus musculus (Q91XE0)
Manually annotated by BRENDA team