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Information on EC 2.3.1.67 - 1-alkylglycerophosphocholine O-acetyltransferase and Organism(s) Mus musculus and UniProt Accession Q8BYI6

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UNIPROT: Q8BYI6 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
lyso-paf-at, acetyl-coa:lyso-paf acetyltransferase, lyso-paf at, lyso-paf:acetyl-coa acetyltransferase, lyso-paf-acetyltransferase, acetyl-coa lyso-paf acetyltransferase, lyso-paf-act, lyso-pafat, lysopafat, lysopaf-at, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholine acetyltransferase
-
acetyl-CoA:lyso-PAF acetyltransferase
-
acetyl-CoA:lysoPAF acetyltransferase
-
lyso-PAF acetyltransferase
-
lyso-PAF acetyltransferase:LPC acyltransferase 1
-
lysoPAFAT
-
lysophosphatidylcholine acyltransferase (LPCAT2)/lyso-PAF acetyltransferase
-
mLysoPAFAT/LPCAT2
-
1-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase
-
-
-
-
acetyl-CoA:1-alkyl-2-lyso-sn-glycero-3-phosphocholine 2-O-acetyltransferase
-
-
-
-
acetyl-CoA:lyso-PAF acetyltransferase
acetyl-coenzyme A:1-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase
-
-
acetyltransferase, 1-alkyl-2-lysolecithin
-
-
-
-
acetyltransferase, 1-alkylglycerophosphocholine
-
-
-
-
acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acyltransferase
-
-
-
-
blood platelet-activating factor acetyltransferase
-
-
-
-
LPCAT1
Ca2+ independent lyso-PAF acetyltransferase
LPCAT2
-
-
lyso-GPC:acetyl CoA acetyltransferase
-
-
-
-
lyso-PAF acetyltransferase
lyso-PAF AT
-
lyso-platelet activating factor:acetyl-CoA acetyltransferase
-
-
-
-
lyso-platelet-activating factor:acetyl-CoA acetyltransferase
-
-
-
-
lysoPAF:acetyl CoA acetyltransferase
-
-
-
-
lysoPAFAT
-
-
lysophosphatidylcholine acyltransferase 1
-
PAF acetyltransferase
-
-
-
-
platelet-activating factor acylhydrolase
-
-
-
-
platelet-activating factor-synthesizing enzyme
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:1-alkyl-sn-glycero-3-phosphocholine 2-O-acetyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
76773-96-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
show the reaction diagram
acetyl-CoA + lyso-platelet-activating factor
platelet-activating factor + CoA
show the reaction diagram
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA
show the reaction diagram
lyso-PAF, lyso-platelet-activating factor
PAF, platelet-activating factor
-
?
acetyl-CoA + 1-hexadecyl-2-lyso-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
acetyl-CoA + 1-octadecyl-2-lyso-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
acetyl-CoA + alk-1-enyl-lyso-glycero-3-phosphoethanolamine
CoA + acetyl-alk-1-enyl-glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
?
acetyl-CoA + octadecyl-lyso-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
show the reaction diagram
acetyl-CoA + lyso-platelet-activating factor
platelet-activating factor + CoA
show the reaction diagram
-
-
-
?
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA
show the reaction diagram
lyso-PAF, lyso-platelet-activating factor
PAF, platelet-activating factor
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
acetyl-CoA
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
dependent on
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-diacetoxy-2-methoxy-5-methyl-3-tetradecanoyloxybenzene
-
2-methoxy-1,3,4-trihydroxy-5-methylbenzene
i.e. 2-methoxy-1,3,4-trihydroxy-5-methylbenzene, isolated from Penicillium sp. strain F33
2-methoxy-5-methyl-1,3,4-triacetoxybenzene
-
2-methoxy-5-methyl-3-octadecanoyloxy-1,4-benzoquinone
-
2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone
-
3,4-dichloro-1-(4,5-dichloro-2-methoxyphenyl)-1H-pyrrole-2,5-dione
-
3,4-dihydroxy-1,2-dimethoxy-5-methylbenzene
-
3-acetoxy-2-methoxy-5-methyl-1,4-benzoquinone
-
3-chloro-1-(3-chloro-4-methoxyphenyl)-4-(2-methoxyphenoxy)-1H-pyrrole-2,5-dione
-
3-chloro-1-(4,5-dichloro-2-methoxyphenyl)-4-(2-hydroxyanilino)-1H-pyrrole-2,5-dione
-
3-chloro-4-(morpholin-4-yl)-1-(4-phenoxyphenyl)-1H-pyrrole-2,5-dione
-
3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
not active against lipoxygenase and cyclooxygenases
3-hexanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
-
3-hydroxy-2-methoxy-5-methyl-1,4-benzoquinone
-
butyl 4-(3,4-dichloro-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoate
83.0% inhibition at 0.02 mM
Decanoic acid
-
dihydrofumigatin
i.e. 2-methoxy-1,3,4-trihydroxy-5-methylbenzene, isolated from Penicillium sp. strain F33
dodecanoic acid
-
methyl 4-(3,4-dichloro-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoate
-
methyl 4-[3-chloro-2,5-dioxo-4-(4-sulfamoylanilino)-2,5-dihydro-1H-pyrrol-1-yl]benzoate
-
myristic acid
-
octanoic acid
-
palmitic acid
-
propan-2-yl 4-(3,4-dichloro-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoate
95.5% inhibition at 0.02 mM
propan-2-yl 4-(3-chloro-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoate
compound competes with acetyl-CoA for the inhibition of isoform LPCAT2 lyso-PAFAT activity and suppresses platelet-activating factor biosynthesis in mouse peritoneal macrophages stimulated with a calcium ionophore. The compound has low inhibitory effects on isoform LPCAT1 activity
propan-2-yl 4-[3-chloro-4-(2-hydroxyanilino)-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl]benzoate
82.4% inhibition at 0.02 mM
propan-2-yl 4-[3-chloro-4-(morpholin-4-yl)-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl]benzoate
-
propyl 4-(3,4-dichloro-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoate
86.7% inhibition at 0.02 mM
2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone
derivative of a metabolite from Penicillium sp. F33 , strong inhibition. Compound also significantly suppresses the gene expression of lyso-PAF acetyltransferase/LPCAT2 in mouse bone marrow-derived macrophages stimulated by lipopolysaccharide
3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
derivative of a metabolite from Penicillium sp. F33 , strong inhibition. Compound also significantly suppresses the gene expression of lyso-PAF acetyltransferase/LPCAT2 in mouse bone marrow-derived macrophages stimulated by lipopolysaccharide
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
lipopolysaccharide
-
activity increases in a time-dependent fashion, maximal activation at 0.010 mg/ml lipopolysaccharide
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1282
1-O-hexadecyl-sn-glycero-3-phosphocholine
apparent KM
0.0824
acetyl-CoA
apparent KM
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
1,4-diacetoxy-2-methoxy-5-methyl-3-tetradecanoyloxybenzene
Mus musculus
pH 6.9, 37°C
0.101
2-methoxy-1,3,4-trihydroxy-5-methylbenzene
Mus musculus
pH 6.9, 37°C
0.5
2-methoxy-5-methyl-1,3,4-triacetoxybenzene
Mus musculus
pH 6.9, 37°C
0.391
2-methoxy-5-methyl-3-octadecanoyloxy-1,4-benzoquinone
Mus musculus
pH 6.9, 37°C
0.02
2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone
Mus musculus
pH 6.9, 37°C
0.166
3,4-dihydroxy-1,2-dimethoxy-5-methylbenzene
Mus musculus
pH 6.9, 37°C
0.121
3-acetoxy-2-methoxy-5-methyl-1,4-benzoquinone
Mus musculus
pH 6.9, 37°C
0.003
3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
Mus musculus
pH 6.9, 37°C
0.139
3-hexanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
Mus musculus
pH 6.9, 37°C
0.109
3-hydroxy-2-methoxy-5-methyl-1,4-benzoquinone
Mus musculus
pH 6.9, 37°C
0.5
Decanoic acid
Mus musculus
pH 6.9, 37°C
0.419
dodecanoic acid
Mus musculus
pH 6.9, 37°C
0.183
myristic acid
Mus musculus
pH 6.9, 37°C
0.5
octanoic acid
Mus musculus
pH 6.9, 37°C
0.5
palmitic acid
Mus musculus
pH 6.9, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000017
brain, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000104
0.000104
spleen, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000470
0.00033
lung, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000452
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
assay at
6.9
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
primary cultured cells of
Manually annotated by BRENDA team
mRNA and protein expressed in
Manually annotated by BRENDA team
experimental allergic encephalomyelitis (EAE) mice, after 12d, 19d, and 34d removed
Manually annotated by BRENDA team
-
peritoneal macrophage
Manually annotated by BRENDA team
mRNA transcript and protein are predominatly expressed in experimental allergic encephalomyelitis (EAE) mice
Manually annotated by BRENDA team
enzymatic activity and mRNA transcripts are elevated in experimental allergic encephalomyelitis (EAE) mice
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
microsomal protein with three putative membrane-spanning domains
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
two entities of lyso-PAFAT belonging to the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family: a constitutively expressed lyso-PAFAT, LPCAT1,and an inducible lyso-PAFAT, LPCAT2. LPCAT1 is mainly expressed in the lungs and produces PAF and dipalmitoyl-phosphatidylcholine, which is a main component of a pulmonary surfactant essential for respiration. LPCAT2 is mainly expressed in inflammatory cells and also possesses biosynthetic activities for PAF and cellular membrane phosphatidylcholine
metabolism
physiological function
physiological function
activities of phospholipase A2 and acetyl-CoA:lyso-PAF acetyltransferase (LysoPAFAT) are elevated in the spinal cord of experimental allergic encephalomyelitis (EAE) mice, an animal model for multiple sclerosis, compared with those of naive mice and correlate with disease severity
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PCAT2_MOUSE
544
1
60254
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
determined by SDS-PAGE and Western Blot analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
stimulation by either platelet-activating factor or ATP results in PKCalpha-mediated phosphorylation of the enzyme at Ser34 causing augmentation of Vmax with minimal Km change. The enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F174A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
I160A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
I162A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
I167A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
I170A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
L166A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
V173A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
V175A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
W163A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
Y169A
mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
LPCAT1 is partially purified from microsomes from mouse tissue using a DEAE-Sepharose column
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Lpcat2, quantitative realtime PCR exprssion analysis
gene LPCAT2, recombinant expression of FLAG-tagged mouse LPCAT2 in CHO cells, stable recombinant enzyme expression in RAW264.7 cells
stable recombinant enzyme expression in RAW264.7 cells, recombinant expression in CHO-S-PAFR cells
for transfection of CHO-K1 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
3-hexanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone, 3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone, and 2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone also inhibit the expression of the enzyme mRNA
fatty acid esters of fumigatin reduce lipopolysaccharide-stimulated transcription of lyso-PAF acetyltransferase/LPCAT2 mRNA in bone marrow-derived macrophages
stimulation of enzyme expression by lipopolysaccharides
under inflammatory conditions, LPCAT2, but not LPCAT1, is activated and upregulated to produce the platelet-activating factor
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
procedure for identification of LPCAT2-specific inhibitors via high-throughput screening
drug development
identification of LPCAT2-specific inhibitors in order to ameliorate PAF-related inflammatory diseases, fluorescence-based high-throughput library screening
medicine
metabolic role in inflammatory disorders using experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS)
medicine
inhibitors 3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone and 2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone at 2.5 mg/kg shod significant, 47.9-51.7%, inhibition in the carrageenan-induced mouse paw edema test, stronger than that of prednisolone at 10 mg/kg
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Svetlov, S.I.; Liu, H.; Chao, W.; Olson, M.S.
Regulation of platelet-activating factor (PAF) biosynthesis via coenzyme A-independent transacylase in the macrophage cell line IC-21 stimulated with lipopolysaccharide
Biochim. Biophys. Acta
1346
120-130
1997
Mus musculus
Manually annotated by BRENDA team
Shindou, H.; Ishii, S.; Yamamoto, M.; Takeda, K.; Akira, S.; Shimizu, T.
Priming effect of lipopolysaccharide on acetyl-Coenzyme A:lyso-platelet-activating factor acetyltransferase is MyD88 and TRIF independent
J. Immunol.
175
1177-1183
2005
Mus musculus
Manually annotated by BRENDA team
Shindou, H.; Hishikawa, D.; Nakanishi, H.; Harayama, T.; Ishii, S.; Taguchi, R.; Shimizu, T.
A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells: cloning and characterization of acetyl-CoA:lyso-PAF acetyltransferase
J. Biol. Chem.
282
6532-6539
2007
Homo sapiens (Q7L5N7), Mus musculus (Q8BYI6)
Manually annotated by BRENDA team
Kihara, Y.; Yanagida, K.; Masago, K.; Kita, Y.; Hishikawa, D.; Shindou, H.; Ishii, S.; Shimizu, T.
Platelet-activating factor production in the spinal cord of experimental allergic encephalomyelitis mice via the group IVA cytosolic phospholipase A2-Lyso-PAFAT axis
J. Immunol.
181
5008-5014
2008
Mus musculus, Mus musculus (Q8BYI6)
Manually annotated by BRENDA team
Harayama, T.; Shindou, H.; Ogasawara, R.; Suwabe, A.; Shimizu, T.
Identification of a novel noninflammatory biosynthetic pathway of platelet-activating factor
J. Biol. Chem.
283
11097-11106
2008
Mus musculus (Q3TFD2), Mus musculus
Manually annotated by BRENDA team
Yamazaki, Y.; Yasuda, K.; Matsuyama, T.; Ishihara, T.; Higa, R.; Sawairi, T.; Yamaguchi, M.; Egi, M.; Akai, S.; Miyase, T.; Ikari, A.; Miwa, M.; Sugatani, J.
A Penicillium sp. F33 metabolite and its synthetic derivatives inhibit acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholine acetyltransferase (a key enzyme in platelet-activating factor biosynthesis) and carrageenan-induced paw edema in mice
Biochem. Pharmacol.
86
632-644
2013
Mus musculus, Mus musculus (Q8BYI6), Rattus norvegicus
Manually annotated by BRENDA team
Morimoto, R.; Shindou, H.; Tarui, M.; Shimizu, T.
Rapid production of platelet-activating factor is induced by protein kinase Calpha-mediated phosphorylation of lysophosphatidylcholine acyltransferase 2 protein
J. Biol. Chem.
289
15566-15576
2014
Mus musculus (Q8BYI6), Mus musculus, Mus musculus C57/BL6J (Q8BYI6)
Manually annotated by BRENDA team
Tarui, M.; Shindou, H.; Kumagai, K.; Morimoto, R.; Harayama, T.; Hashidate, T.; Kojima, H.; Okabe, T.; Nagano, T.; Nagase, T.; Shimizu, T.
Selective inhibitors of a PAF biosynthetic enzyme lysophosphatidylcholine acyltransferase 2
J. Lipid Res.
55
1386-1396
2014
Homo sapiens (Q7L5N7), Mus musculus (Q8BYI6), Mus musculus
Manually annotated by BRENDA team