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Information on EC 2.3.1.64 - agmatine N4-coumaroyltransferase and Organism(s) Hordeum vulgare and UniProt Accession A9ZPJ6

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Hordeum vulgare
UNIPROT: A9ZPJ6 not found.
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The taxonomic range for the selected organisms is: Hordeum vulgare
The enzyme appears in selected viruses and cellular organisms
Synonyms
atact, agmatine coumaroyltransferase, agmatine coumaryl transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agmatine coumaroyltransferase
-
agmatine coumaroyltransferase
-
-
-
-
coumaroyltransferase, agmatine
-
-
-
-
p-coumaroyl-CoA-agmatine N-p-coumaroyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-coumaroyl-CoA:agmatine N4-coumaroyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
85030-72-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-coumaroyl-CoA + agmatine
CoA + 4-coumaroylagmatine
show the reaction diagram
-
-
-
?
caffeoyl-CoA + agmatine
CoA + caffeoylagmatine
show the reaction diagram
-
-
-
?
feruloyl-CoA + agmatine
CoA + feruloyl agmatine
show the reaction diagram
-
-
-
?
4-coumaroyl-CoA + agmatine
CoA + 4-coumaroylagmatine
show the reaction diagram
caffeoyl-CoA + agmatine
CoA + caffeoylagmatine
show the reaction diagram
cinnamoyl-CoA + agmatine
CoA + cinnamoyl agmatine
show the reaction diagram
-
-
-
?
feruloyl-CoA + agmatine
CoA + feruloyl agmatine
show the reaction diagram
sinapoyl-CoA + agmatine
CoA + sinapoyl agmatine
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-coumaroyl-CoA + agmatine
CoA + 4-coumaroylagmatine
show the reaction diagram
-
reaction in hordatine anabolism
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CuSO4
10 mM, 85% inhibition
dithiothreitol
30% inhibition
MnCl2
10 mM, 29% inhibition
ZnSO4
10 mM, 99% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-mercapto-2,3-propanediol
1 mM is required for optimal activity
2-mercaptoethanol
1 mM is required for optimal activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.0021
4-coumaroyl-CoA
0.0052 - 0.0081
agmatine
0.0043 - 0.0058
caffeoyl-CoA
0.0017 - 0.0066
feruloyl-CoA
0.00074 - 0.0015
4-coumaroyl-CoA
0.0095 - 0.012
agmatine
0.0033
caffeoyl-CoA
-
-
0.0033
cinnamoyl-CoA
-
-
0.0013
feruloyl-CoA
-
-
0.05
sinapoyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.34
recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 8.1
half-maximal activity at pH 6.9 and pH 8.1
7 - 8
-
approx. half-maximal activity at pH 7.0 and 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AGCT1_HORVU
439
0
47467
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
1 * 48000, SDS-PAGE
40000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 48000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl
-40°C, concentrated preparation at least 1 month, negligible loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR
ammonium sulfate, Biogel A, Sepharose 4B-agmatine
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bird, C.R.; Smith, T.A.
The biosynthesis of coumaroylagmatine in barley seedlings
Phytochemistry
20
2345-2346
1981
Hordeum vulgare
-
Manually annotated by BRENDA team
Bird, C.R.; Smith, T.A.
Agmatine coumaroyltransferase (barley seedlings)
Methods Enzymol.
94
344-347
1983
Hordeum vulgare
-
Manually annotated by BRENDA team
Bird, C.R.; Smith, T.A.
Agmatine coumaroyltransferase from barley seedlings
Phytochemistry
22
2401-2403
1983
Hordeum vulgare
-
Manually annotated by BRENDA team
Burhenne, K.; Kristensen, B.K.; Rasmussen, S.K.
A new class of N-hydroxycinnamoyltransferases. Purification, cloning, and expression of barley agmatine coumaroyltransferase (EC 2.3.1.64)
J. Biol. Chem.
278
13919-13927
2003
Hordeum vulgare (A9ZPJ6), Hordeum vulgare
Manually annotated by BRENDA team
Nomura, T.; Ishizuka, A.; Kishida, K.; Islam, A.K.; Endo, T.R.; Iwamura, H.; Ishihara, A.
Chromosome arm location of the genes for the biosynthesis of hordatines in barley
Genes Genet. Syst.
82
455-464
2007
Hordeum vulgare (A9ZPJ6), Hordeum vulgare, Triticum aestivum (Q84QG5), Triticum aestivum
Manually annotated by BRENDA team