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Information on EC 2.3.1.54 - formate C-acetyltransferase and Organism(s) Chlamydomonas reinhardtii and UniProt Accession P37836

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.54 formate C-acetyltransferase
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This record set is specific for:
Chlamydomonas reinhardtii
UNIPROT: P37836 not found.
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Word Map
The taxonomic range for the selected organisms is: Chlamydomonas reinhardtii
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
pyruvate formate-lyase, pyruvate formate lyase, pyruvate-formate lyase, pyruvate:formate lyase, formate acetyltransferase, phosphotransferase system enzyme i, formate c-acetyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pyruvate formate-lyase
-
formate acetyltransferase
-
-
-
-
pyruvate formate-lyase
pyruvic formate-lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:formate C-acetyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9068-08-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CoA + pyruvate
acetyl-CoA + formate
show the reaction diagram
CoA + pyruvate
acetyl-CoA + formate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CoA + pyruvate
acetyl-CoA + formate
show the reaction diagram
-
-
-
?
CoA + pyruvate
acetyl-CoA + formate
show the reaction diagram
step in the mitochondrial pathway, overview
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hypophosphite
formate formation from pyruvate inhibited
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
activity assay at, wild-type and mutant protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CC-125 and cell wall-less strain CC-277
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
formate accumulation inside anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured
Manually annotated by BRENDA team
formate accumulation in the medium of anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PFL_CHLRE
195
0
21350
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration, Western Blot, heterologously expressed protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3), pET9a expression vector, transformation into pyruvate formate-lyase-deficient Escherichia coli strain BL21, capacity for formate excretion shown by in vitro tests
gene pfl, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Atteia, A.; van Lis, R.; Gelius-Dietrich, G.; Adrait, A.; Garin, J.; Joyard, J.; Rolland, N.; Martin, W.
Pyruvate formate-lyase and a novel route of eukaryotic ATP synthesis in Chlamydomonas mitochondria
J. Biol. Chem.
281
9909-9918
2006
Chlamydomonas reinhardtii (Q1RS83), Chlamydomonas reinhardtii
Manually annotated by BRENDA team
Hemschemeier, A.; Jacobs, J.; Happe, T.
Biochemical and physiological characterization of the pyruvate formate-lyase Pfl1 of Chlamydomonas reinhardtii, a typically bacterial enzyme in a eukaryotic alga
Eukaryot. Cell
7
518-526
2008
Chlamydomonas reinhardtii (P37836), Chlamydomonas reinhardtii
Manually annotated by BRENDA team