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Information on EC 2.3.1.51 - 1-acylglycerol-3-phosphate O-acyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P33333

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EC Tree
IUBMB Comments
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P33333
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
abhd5, agpat2, lpaat, lysophosphatidic acid acyltransferase, lpaat-beta, agpat1, 1-acylglycerol-3-phosphate o-acyltransferase, agpat3, lpa acyltransferase, agpat4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-acyl-sn-glycerol-3-phosphate acyltransferase
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1-acylglycerol 3-phosphate O-acyltransferase
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lysophosphatidic acid acyltransferase
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lysophospholipid:acyl-CoA acyltransferase
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1-acyl-sn-glycero-3-phosphate acyltransferase
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1-acyl-sn-glycerol 3-phosphate acyltransferase
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1-acyl-sn-glycerol-3-phosphate acyltransferase
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1-acylglycero-3-phosphate acyltransferase
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1-acylglycerolphosphate acyltransferase
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1-acylglycerophosphate acyltransferase
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acyltransferase, 1-acylglycerol phosphate
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ICT1 protein
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lysophosphatidic acid ayltransferase
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lysophosphatidic acid-acyltransferase
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oleoyl-CoA lysophosphatidic acid acyltransferase
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additional information
cf. EC 2.3.1.121 and EC 2.3.1.23
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
51901-16-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 1-oleoyl-lysophosphatidic acid
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid
CoA + 1,2-dioleoyl-sn-lysophosphatidic acid
show the reaction diagram
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine
CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine
show the reaction diagram
low activity, cf. EC 2.3.1.23. Slc1 has no detectable reverse reaction towards PtdCho substrate
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-
ir
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine
CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine
show the reaction diagram
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
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the rate of CoA-liberation is much lower than with 1-acyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
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substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA
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-
?
oleoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
acyltranferase activity by Slc1p and Slc4p
-
-
?
acyl-CoA + 1-oleoyl-lysophosphatidic acid
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid
show the reaction diagram
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-
-
?
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid
CoA + 1,2-dioleoyl-sn-lysophosphatidic acid
show the reaction diagram
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-
-
r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine
CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine
show the reaction diagram
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-
-
r
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
additional information
?
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comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview. The yeast LPAAT Slc1p or ScLPAAT has been shown to have activity with 22:1-CoA in addition to 18:1-CoA substrates, indicating a broad substrate specificity with respect to chain length although 22:6-CoA, 22:5-CoA, and other LC-PUFAs has not been tested as substrates
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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activity assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the most conserved motif containing the presumed active site histidine of isoform Slc1 is oriented in the the membrane towards the lumen, whereas other conserved motifs are cytosolic.embrane to the endereas other conserved motifs are cytosolic
Manually annotated by BRENDA team
and endoplasmic reticulum
Manually annotated by BRENDA team
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the presence of the enzyme in the mitochondrial fraction cannot be explained by the contamination of microsomes in this fraction
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
strains deficient for the enzyme show abnormalities in lipid droplet morphology and contain more lipid droplets, while overexpression results in fewer lipid droplets. Mutant strains reveal a significant reduction in triacylglycerol content, while phospholipid composition remains unchanged. Both phopholipids and glycerolipids are qualitatively affected by the mutation
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61180
partially purified Slc1p, SDS-PAGE, Western Blot
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partially purified, gel filtration
gel filtration and SDS-PAGE, recombinant protein
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partially purified, gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain HB101, transformed into Saccharomyces cerevisiae, strains, plasmids and sources of parent vectors listed
gene Slc1, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method
expressed in Escherichia coli strain HB101, transformed into Saccharomyces cerevisiae, strains, plasmids and sources of parent vectors listed
expressed in Escherichia coli, overexpression in Saccharomyces cerevisiae, expression plasmid pPS189
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expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamada, K.; Okuyama, H.; Endo, Y.; Ikezawa, H.
Acyltransferase systems involved in phospholipid metabolism in Saccharomyces cerevisiae
Arch. Biochem. Biophys.
183
281-289
1977
Saccharomyces cerevisiae, Saccharomyces cerevisiae OC-2
Manually annotated by BRENDA team
Benghezal, M.; Roubaty, C.; Veepuri, V.; Knudsen, J.; Conzelmann, A.
SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast
J. Biol. Chem.
282
30845-30855
2007
Saccharomyces cerevisiae (P33333), Saccharomyces cerevisiae (Q08548), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Ghosh, A.K.; Ramakrishnan, G.; Rajasekharan, R.
YLR099C (ICT1) encodes a soluble Acyl-CoA-dependent lysophosphatidic acid acyltransferase responsible for enhanced phospholipid synthesis on organic solvent stress in Saccharomyces cerevisiae
J. Biol. Chem.
283
9768-9775
2008
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Pagac, M.; de la Mora, H.V.; Duperrex, C.; Roubaty, C.; Vionnet, C.; Conzelmann, A.
Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae
J. Biol. Chem.
286
36438-36447
2011
Saccharomyces cerevisiae (P33333), Saccharomyces cerevisiae (Q08548), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Ayciriex, S.; Le Guedard, M.; Camougrand, N.; Velours, G.; Schoene, M.; Leone, S.; Wattelet-Boyer, V.; Dupuy, J.W.; Shevchenko, A.; Schmitter, J.M.; Lessire, R.; Bessoule, J.J.; Testet, E.
YPR139c/LOA1 encodes a novel lysophosphatidic acid acyltransferase associated with lipid droplets and involved in TAG homeostasis
Mol. Biol. Cell
23
233-246
2012
Saccharomyces cerevisiae (Q06508), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Shrestha, P.; Hussain, D.; Mulder, R.J.; Taylor, M.C.; Singh, S.P.; Petrie, J.R.; Zhou, X.R.
Increased DHA production in seed oil using a selective lysophosphatidic acid acyltransferase
Front. Plant Sci.
9
1234
2018
Mortierella alpina, Yarrowia lipolytica (A0A1D8NJ27), Micromonas pusilla (C1MQM1), Saccharomyces cerevisiae (P33333), Limnanthes alba (Q42868), Arabidopsis thaliana (Q8LG50), Brassica napus (Q9LLY4), Emiliania huxleyi (R1BZP3), Emiliania huxleyi
Manually annotated by BRENDA team
Jasieniecka-Gazarkiewicz, K.; Demski, K.; Lager, I.; Stymne, S.; Banas, A.
Possible role of different yeast and plant lysophospholipid acyl-CoA acyltransferases (LPLATs) in acyl remodelling of phospholipids
Lipids
51
15-23
2016
Saccharomyces cerevisiae (P33333), Saccharomyces cerevisiae
Manually annotated by BRENDA team