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Information on EC 2.3.1.50 - serine C-palmitoyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LSZ9

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IUBMB Comments
A pyridoxal-phosphate protein.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LSZ9
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
serine palmitoyltransferase, sptlc1, sptlc2, serine palmitoyl transferase, ssspta, serine-palmitoyltransferase, serine-palmitoyl transferase, serine palmitoyltransferase a, ssspt, serine c-palmitoyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
serine palmitoyltransferase
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3-oxosphinganine synthetase
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-
-
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acyl-CoA:serine C-2 acyltransferase decarboxylating
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-
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LCB2a
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subunit
LCB2b
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subunit
palmitoyltransferase, serine
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-
-
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serine palmitoyltransferase
serine-palmitoyltransferase
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-
-
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ssSPT
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small subunit
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
-
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Acyl group transfer
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-
-
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PATHWAY SOURCE
PATHWAYS
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-, -, -
SYSTEMATIC NAME
IUBMB Comments
palmitoyl-CoA:L-serine C-palmitoyltransferase (decarboxylating)
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-50-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
palmitoyl-CoA + L-serine
CoA + 3-dehydro-D-sphinganine + CO2
show the reaction diagram
palmitoyl-CoA + L-serine
CoA + 3-dehydro-D-sphinganine + CO2
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
palmitoyl-CoA + L-serine
CoA + 3-dehydro-D-sphinganine + CO2
show the reaction diagram
step in the sphingolipid biosynthetic pathway, overview
-
-
?
palmitoyl-CoA + L-serine
CoA + 3-dehydro-D-sphinganine + CO2
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
mycotoxin fumonisin B1
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enzyme sensitivity to the inhibitor is increased by small subunit ssSPTa overexpression
additional information
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orosomucoid proteins ORM1 and ORM2 inhibit the function of the small subunit ssSP and thus negatively regulate enzyme activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
small subunits of serine palmitoyltransferase
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isoforms ssSPTa and ssSPTb, UniProt A8MSB8 and F4IPU5, strongly stimulate SPT activity. Small subunit ssSPTa transcripts are more enriched in all organs and over 400fold more abundant in pollen than small subunit ssSPTb transcripts. Homozygous ssSPTa T-DNA mutants are not recoverable, and 50% nonviable pollen is detected in heterozygous ssspta mutants. The small subunits are essential for male gametophytes, are important for mycotoxin fumonisin B1 sensitivity, and limit sphingolipid synthesis in planta
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression of LCB1
Manually annotated by BRENDA team
young, expression of LCB1
Manually annotated by BRENDA team
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small subunit ssSPTa transcripts are more enriched in all organs and over 400fold more abundant in pollen than small subunit ssSPTb transcripts
Manually annotated by BRENDA team
expression of LCB1
Manually annotated by BRENDA team
expression of LCB1
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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homozygous ssSPTa T-DNA mutants are not recoverable, and 50% nonviable pollen is detected in heterozygous ssSpta mutants. Pollen viability is recovered by expression of wild-type ssSPTa or ssSPTb under control of the ssSPTa promoter, indicating ssSPTa and ssSPTb functional redundancy. SPT activity and sensitivity to the PCD-inducing mycotoxin fumonisin B1 are increased by ssSPTa overexpression. Conversely, SPT activity and mycotoxin fumonisin B1 sensitivity are reduced in ssSPTa RNA interference lines
metabolism
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serine palmitoyltransferase, composed of LCB1 and LCB2 subunits, catalyzes the primary regulatory point for sphingolipid synthesis
physiological function
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enzyme activators, the small subunits are essential for male gametophytes, are important for mycotoxin fumonisin B1 sensitivity, and limit sphingolipid synthesis in planta
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LCB2A_ARATH
489
1
54290
Swiss-Prot
Secretory Pathway (Reliability: 3)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
dimer
the enzyme is formed by two subunits, LCB1 and LCB2, i.e. long-chain base 1 and 2
heterodimer
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serine palmitoyltransferase is composed of LCB1 and LCB2 subunits
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
subunits LCB1 and LCB2 expression analysis
subunits LCB1 and LCB2, phylogenetic tree, expression of Arabidopsis thaliana subunit LCB1 rescues the sphingolipid long-chain base auxotrophy of Saccharomyces cerevisiae SPT mutants when coexpressed with Arabidopsis thaliana LCB2
recombinant expression of FLAG-tagged enzyme subunit LCB1 and of Myc-tagged enzyme subunit LCB2 in Saccharomyces cerevisiae microsomes. Expressing the Arabidopsis thaliana LCB1/LCB2 heterodimers has very low SPT activity, whereas coexpression of Arabidopsis thaliana ssSPTs, ssSPTa and ssSPTb, derived 56-amino acid polypeptides in a Saccharomyces cerevisiae enzyme SPT null mutant and stimulate SPT enzyme activity of the Arabidopsis LCB1/LCB2 heterodimer by over 100fold through physical interaction with LCB1/LCB2. Transient Agrobacterium tumefaciens-mediated transformation of Nicotiana benthaminana with YFP-labeled small subunits of serine palmitoyltransferase, ssSPTa and ssSPTb
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subunits LCB1 and LCB2 expression analysis
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
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results show that SPT modulated programmed cell death plays an important role in the regulation of male gametophyte development of Arabidopsis thaliana
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chen, M.; Han, G.; Dietrich, C.R.; Dunn, T.M.; Cahoon, E.B.
The essential nature of sphingolipids in plants as revealed by the functional identification and characterization of the Arabidopsis LCB1 subunit of serine palmitoyltransferase
Plant Cell
18
3576-3593
2006
Arabidopsis thaliana (Q9LSZ9), Arabidopsis thaliana
Manually annotated by BRENDA team
Teng, C.; Dong, H.; Shi, L.; Deng, Y.; Mu, J.; Zhang, J.; Yang, X.; Zuo, J.
Serine palmitoyltransferase, a key enzyme for de novo synthesis of sphingolipids, is essential for male gametophyte development in Arabidopsis
Plant Physiol.
146
1322-1332
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Gan, Y.; Zhang, L.; Zhang, Z.; Dong, S.; Li, J.; Wang, Y.; Zheng, X.
The LCB2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and Bax-induced cell death
New Phytol.
181
127-146
2009
Arabidopsis thaliana (Q94IB8), Arabidopsis thaliana (Q9LSZ9), Brassica rapa subsp. oleifera (B5LED9)
Manually annotated by BRENDA team
Kimberlin, A.N.; Majumder, S.; Han, G.; Chen, M.; Cahoon, R.E.; Stone, J.M.; Dunn, T.M.; Cahoon, E.B.
Arabidopsis 56-amino acid serine palmitoyltransferase-interacting proteins stimulate sphingolipid synthesis, are essential, and affect mycotoxin sensitivity
Plant Cell
25
4627-4639
2013
Arabidopsis thaliana
Manually annotated by BRENDA team
Li, J.; Yin, J.; Rong, C.; Li, K.E.; Wu, J.X.; Huang, L.Q.; Zeng, H.Y.; Sahu, S.K.; Yao, N.
Orosomucoid proteins interact with the small subunit of serine palmitoyltransferase and contribute to sphingolipid homeostasis and stress responses in Arabidopsis
Plant Cell
28
3038-3051
2016
Arabidopsis thaliana
Manually annotated by BRENDA team