type A enzymes are localized in the nuclei and acetylate nucleosomal histones as well as nonhistone proteins, type B enzymes can be found in the cytoplasmic fraction and are responsible for acetylation of newly synthesized histones before their translocation into the nucleus for chromatin assembly during DNA replication
acetylation at Lys16 by MYST1 is essential for chromatin remodeling and is used for regulation of gene expression in eukaryotes. The nucleosome is a disc-shaped octamer consisting of two heterotetramers formed by histones H3/H4 and histones H2A and H2B
Mof is required for sex chromosome dosage compensation acting in the MSL complex, which also contains Msl1-3, Mle, and RNA, to acetylate H4K16 and to increase gene transcription from the single male X chromosome
the enzyme plays a role in chromatin structure and gene expression regulation as a catalytic component of multiprotein complexes, some of which also contain Ada2-type transcriptional coactivators
MYST1 is a part of multiprotein complexes that accomplish functions of male X chromosome activation and thereby functions of dosage compensation in Drosophila and, in mammals, global acetylation of histone H4 K16. Functional links between MYST1 and proteins ATM and p53. Interactions between MSL1 and MYST1 within the MSL complex in Drosophila melanogaster, the compensasome includes proteins MSL1, MSL2, MSL3, MLE, MOF, a histone acetyltransferase homologous to MYST1, JIL1, and two non-coding RNA: roX1 and roX2, structure and function of the compensasome, detailed overview. Cell interactome fragments including protein homologs of hampin and MYST1, overview
acetylation at Lys16 by MYST1 is essential for chromatin remodeling and is used for regulation of gene expression in eukaryotes. The nucleosome is a disc-shaped octamer consisting of two heterotetramers formed by histones H3/H4 and histones H2A and H2B
Mof is required for sex chromosome dosage compensation acting in the MSL complex, which also contains Msl1-3, Mle, and RNA, to acetylate H4K16 and to increase gene transcription from the single male X chromosome
the enzyme plays a role in chromatin structure and gene expression regulation as a catalytic component of multiprotein complexes, some of which also contain Ada2-type transcriptional coactivators
MYST1 is a part of multiprotein complexes that accomplish functions of male X chromosome activation and thereby functions of dosage compensation in Drosophila and, in mammals, global acetylation of histone H4 K16. Functional links between MYST1 and proteins ATM and p53. Interactions between MSL1 and MYST1 within the MSL complex in Drosophila melanogaster, the compensasome includes proteins MSL1, MSL2, MSL3, MLE, MOF, a histone acetyltransferase homologous to MYST1, JIL1, and two non-coding RNA: roX1 and roX2, structure and function of the compensasome, detailed overview. Cell interactome fragments including protein homologs of hampin and MYST1, overview
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
the enzyme belongs to the MYST family. The MYST family takes its name from the first identified members: (MOZ, KAT6A), Ybf2 (Sas3, KAT6), something about silencing (Sas2, KAT8) and Tat-interacting protein (Tip60, KAT5). To date, five human KATs have been identified in this family: MOZ, MOZ related factor (MORF, KAT6B), Tip60, HAT bound to ORC1 (HBO1, KAT7) and males absent on the first (MOF, KAT8 or MYST 1), the functional orthologue of yeast's Sas2. The defining feature of MYST family is the presence of the highly conserved MYST domain. MYST enzymes possess a highly-conserved acetyl-CoA-binding motif A within the catalytic domain. Additionally, some family members have also structural features in common with one another, such as chromodomains or plant homeodomain-linked zinc fingers. The members of this family utilize a double displacement (or ping-pong) catalytic mechanism. Autoacetylation is an important process in modulating the activity of MYST family members
lysine acetylation is a post-translational modification of both histone and nonhistone proteins that is catalyzed by lysine acetyltransferases and plays a key role in numerous biological contexts. The MYST protein MOF catalyzes the acetylation of p53 at lysine120, which helps to discriminate cell-cycle arrest and apoptotic functions. MYST enzymes have their acetylase activity regulated by autoacetylation
the enzyme belongs to the MYST family. The MYST family takes its name from the first identified members: (MOZ, KAT6A), Ybf2 (Sas3, KAT6), something about silencing (Sas2, KAT8) and Tat-interacting protein (Tip60, KAT5). To date, five human KATs have been identified in this family: MOZ, MOZ related factor (MORF, KAT6B), Tip60, HAT bound to ORC1 (HBO1, KAT7) and males absent on the first (MOF, KAT8 or MYST 1), the functional orthologue of yeast's Sas2. The defining feature of MYST family is the presence of the highly conserved MYST domain. MYST enzymes possess a highly-conserved acetyl-CoA-binding motif A within the catalytic domain. Additionally, some family members have also structural features in common with one another, such as chromodomains or plant homeodomain-linked zinc fingers. The members of this family utilize a double displacement (or ping-pong) catalytic mechanism. Autoacetylation is an important process in modulating the activity of MYST family members
genome-wide increase in histone acetylation stimulates replication independently of transcription in follicle cells. Enok is essential for mushroom body development, the mushroom bodies are the sites of olfactory learning and memory and in this function equivalent to the mammalian brain. Mof is required for sex chromosome dosage compensation acting in the MSL complex
targeted reduction of ELP3 specifically in the developing Drosophila nervous system leads to a hyperactive phenotype with increase in climbing and locomotor activities and sleep loss in the adult flies, a significant expansion in synaptic bouton number and axonal length and branching in the larval neuromuscular junction as well as the misregulation of genes involved in sleep, vesicle transport and fusion, and protein chaperone activity. Ubiquitous reduction of ELP3 results in lethality
regulation of histone acetylation is fundamental to the utilization of eukaryotic genomes in chromatin. H4K16 acetylation is thought to affect the basic properties of the chromatin fiber. Male cells display twice the amount of H4K16 acetylation but reduced levels of several other acetylation motifs including H4K5, H4K12, and H3K14 acetylation as compared to female cells due to acetyltransferase MOF. Drosophila's histone acetylation system includes (1) the extensively studied model KATs (GCN5/PCAF, CBP/P300, MOF, HAT1, and TIP60), (2) less well characterized KATs (KAT6 [MOZ/MORF], HBO1, ELP3, TAF1, and ATAC2), (3) a mostly uncharacterized class of GCN5-related KATs (NAT6, NAT9, and NAT10), (4) N-terminal acetyltransferases suggested to also acetylate internal lysines (NAA10, NAA20, NAA30, NAA40, NAA50, and NAA60), (5) putative acetyltransferases with no recognizable direct homologue in non-Drosophilid species (CG5783 and CG12560), (6) the acetyltransferase ECO, and (7) a bifunctional enzyme containing a O-GlcNAcase activity and potentially a KAT activity (MGEA5, also known as NCOAT or OGA)
lysine acetylation is a post-translational modification of both histone and nonhistone proteins that is catalyzed by lysine acetyltransferases and plays a key role in numerous biological contexts. MYST enzymes have their acetylase activity regulated by autoacetylation. Tip60 activates the machinery for DNA repair
lysine acetylation is a post-translational modification of both histone and nonhistone proteins that is catalyzed by lysine acetyltransferases and plays a key role in numerous biological contexts. TAF1 is a regulatory element in hormone-related transcriptional processes
mechanism of action and structural analysis of KATs, detailed overview. All KATs are characterized by a similar tertiary structure in the central core. This structure consists of an alpha/beta fold, which is important for co-substrate binding and catalysis
mechanism of action and structural analysis of KATs, detailed overview. All KATs are characterized by a similar tertiary structure in the central core. This structure consists of an alpha/beta fold, which is important for co-substrate binding and catalysis
mechanism of action and structural analysis of KATs, detailed overview. All KATs are characterized by a similar tertiary structure in the central core. This structure consists of an alpha/beta fold, which is important for co-substrate binding and catalysis
development of a quantitative proteomic strategy to generate a comprehensive catalog of combinatorial histone acetylation and methylation motifs in Drosophila cells, acetylation patterns and their genesis by integrated enzyme activities, e.g. via enzymes MOF, RPD3, KAT6, NAA10, and GCN5, overview
mechanism of action and structural analysis of KATs, detailed overview. All KATs are characterized by a similar tertiary structure in the central core. This structure consists of an alpha/beta fold, which is important for co-substrate binding and catalysis
mechanism of action and structural analysis of KATs, detailed overview. All KATs are characterized by a similar tertiary structure in the central core. This structure consists of an alpha/beta fold, which is important for co-substrate binding and catalysis
mechanism of action and structural analysis of KATs, detailed overview. All KATs are characterized by a similar tertiary structure in the central core. This structure consists of an alpha/beta fold, which is important for co-substrate binding and catalysis
MYST enzymes have their acetylase activity regulated by autoacetylation. Autoacetylation is an important process in modulating the activity of MYST family members
MYST enzymes have their acetylase activity regulated by autoacetylation. Autoacetylation is an important process in modulating the activity of MYST family members
site-directed mutagenesis of the Gn5 gene, inactive mutant, the loss of either dGcn5 or dAda2a function results in similar chromosome structural and developmental defects, in dAda2a mutants, the nucleosomal H4 acetylation at lysines 12 and 5 is significantly reduced, while the acetylation established by dAda2b-containing Gcn5 complexes at H3 lysines 9 and 14 is unaffected, overview
Both an enok null allele and a point mutation in the zinc finger of the MYST histone acetyltransferase domain cause an arrest in neuroblast proliferation. Enok mutation also results in a slow, but steady decline of egg production over time. However, no difference occurs in the proliferation of wing disk cells. Effects of mutating mof in flies are certainly most pronounced at and most relevant to the male X chromosome. Null mutation of the Hbo1 homologue in Drosophila melanogaster, chameau, leads to lethality at the pupal stage. Haploinsufficiency for chameau leads to defects in position effect variegation, in polycomb group protein mediated repression of homeotic genes and consequent homeotic transformation in thoracic and abdominal segments. Compound heterozygous flies for chameau and the PcG genes polycomb, polyhomeotic, or a mutation in a polycomb response element, Mcp, show more pronounced homeotic transformation than heterozygotes of the PcG genes alone. Depletion of Tip60 by RNAi leads to developmental lethality before or at the early pupal stage
MOF levels are strongly diminished after incubating cells for 4 days with interfering RNAs directed against MOF transcript, and are undetectable after 5.5 days