Information on EC 2.3.1.330 - D-xylulose 5-phosphate:acyl-carrier protein glycolyltransferase system

This enzyme system produces glycolyl-[acp] units that can be used by non-ribosomal peptide synthases. It catalyses a transketolase-like reaction on ketose phosphates derived from primary metabolism and transfers the resulting glycolyl moiety to a dedicated acyl-carrier protein. During the reaction cycle the glycolyl moiety is transferred first to a thiamine diphosphate cofactor, then to a lipoyl cofactor, and eventually to the acyl-carrier protein. While D-xylulose 5-phosphate is the best substrate, the enzyme can also accept D-fructose 6-phosphate and D-sedheptulose 7-phosphate. During the reaction the lipoyl cofactor is reduced to dihydrolipoyl, which must be oxidized back to lipoyl by an unknown enzyme.