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Information on EC 2.3.1.294 - meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II
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EC Tree
2.3.1.294 meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase IIIUBMB Comments
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasB differs from KasA (EC 2.3.1.293, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I), by preferring longer substrates (closer to the upper limit), which also contain two double bonds.
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
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an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein]
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an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein]
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Synonyms
beta-ketoacyl-acyl carrier protein synthase b, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-ketoacyl AcpM synthase
beta-ketoacyl acyl carrier protein synthase II
beta-ketoacyl-ACP synthase
beta-ketoacyl-acyl carrier protein synthase
beta-ketoacyl-acyl carrier protein synthase B
beta-ketoacyl-acyl carrier protein synthase KasB
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-
-
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beta-ketoacyl-acyl-carrier protein synthase
beta-ketoacylacyl synthase
KasB
Rv2246
beta-ketoacyl-acyl carrier protein synthase
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KasB
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KasB
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(Z)-hexadec-11-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein]
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-
-
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(Z)-hexadec-11-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
active site structure with the Cys-His-His active site triad and the acyl binding channel, reaction mechanism, modeling
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(Z)-hexadec-11-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
active site structure with the Cys-His-His active site triad and the acyl binding channel, reaction mechanism, modeling
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(Z)-hexadec-11-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ultra-long-chain di-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasB differs from KasA (EC 2.3.1.293, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I), by preferring longer substrates (closer to the upper limit), which also contain two double bonds.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein AcpM]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
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-
-
-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + palmitoyl-[acyl-carrier protein AcpM]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
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-
-
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?
C12:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli]
?
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very low activity
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-
?
C20:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli]
?
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very low activity
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-
?
C4:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli]
?
-
very low activity
-
-
?
lauroyl-CoA + malonyl-CoA
?
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2% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli]
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?
lauroyl-CoA + malonyl-phosphopantetheine
?
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1% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli]
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-
?
lauroyl-CoA + malonyl-phosphopantetheine 14-mer
?
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108% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli]
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-
?
lauroyl-CoA + malonyl-phosphopantetheine 16-mer
?
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100% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli]
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-
?
lauroyl-CoA + malonyl-phosphopantetheine 8-mer
?
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22% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli]
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?
lauroyl-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli]
?
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100% activity
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?
myristoyl-[acyl-carrier protein] + biotinylated malonyl-[acyl-carrier protein]
?
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?
palmitoyl-CoA + malonyl-[acyl-carrier protein from Escherichia coli]
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weak activity
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?
palmitoyl-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli]
?
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best substrate
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?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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the enzyme synthesizes longer multi-unsaturated acyl chains averaging 54 carbons units
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?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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-
-
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?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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C38-C42
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-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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C38-C42
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-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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?
palmitoyl-CoA + a malonyl-[acyl-carrier protein]
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?
palmitoyl-CoA + malonyl-[acyl-carrier protein AcpM]
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?
palmitoyl-[acyl-carrier protein M] + malonyl-[acyl-carrier protein M]
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?
palmitoyl-[acyl-carrier protein M] + malonyl-[acyl-carrier protein M]
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?
additional information
?
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the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall
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?
additional information
?
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the enzyme catalyzes the Claisen-type condensation reaction
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?
additional information
?
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the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall
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?
additional information
?
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the enzyme catalyzes the Claisen-type condensation reaction
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein AcpM]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
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-
-
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?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + palmitoyl-[acyl-carrier protein AcpM]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
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-
-
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?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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the enzyme synthesizes longer multi-unsaturated acyl chains averaging 54 carbons units
-
-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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-
-
-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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C38-C42
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-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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C38-C42
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-
?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
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-
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-
?
additional information
?
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the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall
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-
?
additional information
?
-
-
the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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non-ionic detergents such as n-octyl-beta-D-glucopyranoside have no positive effect on enzyme activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.005
biotinylated malonyl-[acyl-carrier protein]
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at pH 7.0 and 37°C
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0.0019
C20:0-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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0.0061
malonyl-phosphopantetheine 14-mer
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wild type enzyme, at pH 8.5 and 25°C
0.0062
malonyl-phosphopantetheine 16-mer
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wild type enzyme, at pH 8.5 and 25°C
0.0237
malonyl-phosphopantetheine 8-mer
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wild type enzyme, at pH 8.5 and 25°C
0.0135
malonyl-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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0.0014
palmitoyl-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
C20:0-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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0.023
malonyl-phosphopantetheine 14-mer
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wild type enzyme, at pH 8.5 and 25°C
0.022
malonyl-phosphopantetheine 16-mer
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wild type enzyme, at pH 8.5 and 25°C
0.018
malonyl-phosphopantetheine 8-mer
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wild type enzyme, at pH 8.5 and 25°C
0.023
malonyl-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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0.027
palmitoyl-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.33
C20:0-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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3.83
malonyl-phosphopantetheine 14-mer
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wild type enzyme, at pH 8.5 and 25°C
0.022
malonyl-phosphopantetheine 16-mer
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wild type enzyme, at pH 8.5 and 25°C
4.17
malonyl-phosphopantetheine 8-mer
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wild type enzyme, at pH 8.5 and 25°C
1.67
malonyl-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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18.33
palmitoyl-[acyl-carrier protein from Escherichia coli]
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at pH 7.0 and 37°C
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0318
-
palmitoyl-[acyl-carrier protein] as substrate, at pH 7.0 and 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 42
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the enzyme displays about 2-3fold higher specific activities at 37°C compared with 30 or 42°C
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
brenda
-
-
-
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
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an inactive enzyme-deficient mutant has less density of mycolic acid, embedded in periplasmic space, with normal thickness in the cell envelope, which results in loss of acid-fastness
malfunction
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enzyme gene deletion in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice. An additional outcome of enzyme gene deletion is the loss of ketomycolic acid trans-cyclopropanation and a drastic reduction in methoxymycolic acid trans-cyclopropanation. Although deletion of the enzyme also markedly alters the colony morphology and abolishes classic serpentine growth (cording), the most profound effect of enzyme gene deletion is the ability of the mutant strain to persist in infected immunocompetent mice for up to 600 days without causing disease or mortality
malfunction
-
enzyme mutants exhibit strikingly altered cell wall permeability, leading to a marked increase in susceptibility to lipophilic antibiotics and the host antimicrobial molecules defensing and lysozyme. The predominant chain lengths are 2-4 carbons shorter for oxygenated mycolates in the absence of the enzyme
malfunction
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an inactive enzyme-deficient mutant has less density of mycolic acid, embedded in periplasmic space, with normal thickness in the cell envelope, which results in loss of acid-fastness
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malfunction
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enzyme mutants exhibit strikingly altered cell wall permeability, leading to a marked increase in susceptibility to lipophilic antibiotics and the host antimicrobial molecules defensing and lysozyme. The predominant chain lengths are 2-4 carbons shorter for oxygenated mycolates in the absence of the enzyme
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metabolism
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the enzyme can catalyze both saturated and unsaturated fatty acid biosynthesis
metabolism
-
the enzyme is involved in fatty acid and mycolic acid biosynthesis
metabolism
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when enzyme KasB is coexpressed with KasA, it appears to be capable of facilitating the production of lipids of the length of full meromycolic acids. KasB therefore may accept primers directly from KasA that average 40 carbons in length. KasA and KasB thus function in tandem to carrier out acyl chain elongation to achieve meromycolic acid synthesis from acyl primers provided by type I fatty acid synthase system
metabolism
-
the enzyme is involved in fatty acid and mycolic acid biosynthesis
-
metabolism
-
when enzyme KasB is coexpressed with KasA, it appears to be capable of facilitating the production of lipids of the length of full meromycolic acids. KasB therefore may accept primers directly from KasA that average 40 carbons in length. KasA and KasB thus function in tandem to carrier out acyl chain elongation to achieve meromycolic acid synthesis from acyl primers provided by type I fatty acid synthase system
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physiological function
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enzyme-dependent mycolate elongation is essential for cording and cell wall impermeability. Enzyme-dependent cell wall impermeability is essential for antibiotic resistance. The enzyme is essential for resistance to macrophage antimicrobial activity
physiological function
-
the enzyme is involved in cording and acid-fast staining and is required for survival and virulence in infected mice
physiological function
-
enzyme-dependent mycolate elongation is essential for cording and cell wall impermeability. Enzyme-dependent cell wall impermeability is essential for antibiotic resistance. The enzyme is essential for resistance to macrophage antimicrobial activity
-
physiological function
-
the enzyme is involved in cording and acid-fast staining and is required for survival and virulence in infected mice
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). KASB_MYCBO
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
417
0
44277
Swiss-Prot
-
KASB_MYCTO
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
417
0
44277
Swiss-Prot
-
KASB_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
417
0
44277
Swiss-Prot
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
88000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
homodimer
dimer
-
homodimer, crystal structure, mtKasB adopts a thiolase fold but contains unique structural features in the capping region that may be crucial to its preference for longer fatty acyl chains than its counterparts from other bacteria, residues 1-260 and 261-415 form topologically equivalent N and C-terminal betaalphabetaalphabetaalphabetabeta motifs, overview, structure comparisons
dimer
-
homodimer, crystal structure, mtKasB adopts a thiolase fold but contains unique structural features in the capping region that may be crucial to its preference for longer fatty acyl chains than its counterparts from other bacteria, residues 1-260 and 261-415 form topologically equivalent N and C-terminal betaalphabetaalphabetaalphabetabeta motifs, overview, structure comparisons
-
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
phosphoprotein
-
phosphorylation increases the activity of the enzyme in the elongation process of long chain fatty acids synthesis
phosphoprotein
-
phosphorylation of the enzyme at Thr334 and Thr336 profoundly decreases the condensing activity of the enzyme. Phosphorylation of the enzyme leads to increased cell wall permeability
phosphoprotein
-
transphosphorylation increases the enzyme activity
phosphoprotein
-
phosphorylation increases the activity of the enzyme in the elongation process of long chain fatty acids synthesis
-
phosphoprotein
-
transphosphorylation increases the enzyme activity
-
phosphoprotein
-
phosphorylation of the enzyme at Thr334 and Thr336 profoundly decreases the condensing activity of the enzyme. Phosphorylation of the enzyme leads to increased cell wall permeability
-
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with palmitoyl-CoA, sitting drop vapor diffusion method, using 100 mM CAPS pH 10.5, 20 % w/v PEG 8000, 200 mM NaCl, spermine hydrochloride and foscholine-9
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purified recombinant enzyme, sitting drop vapour diffusion method, from 100 mM Caps, pH 10.5, 20% w/v PEG 8000, 200 mM NaCl, and 5 mM C16-CoA, with spermidine-HCl and Foscholine-9 detergent added, X-ray diffraction crystal structure determination and analysis at 2.4-3.0 A resolution, molecular replacement, structure modeling
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
R206G
-
the mutant shows a 10fold increase in kcat/Km when malonyl-CoA is varied at a fixed concentration of lauroyl-CoA (0.1 mM)
T334D/T336D
-
the phosphomimetic mutant exhibits loss of acid-fast staining and is more susceptible to isoniazid, ethionamide and rifampicin compared to the wild type enzyme
T334D/T336D
-
the phosphomimetic mutant exhibits loss of acid-fast staining and is more susceptible to isoniazid, ethionamide and rifampicin compared to the wild type enzyme
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione-Sepharose 4B column chromatography and Ni-NTA agarose bead chromatography
-
Ni2+-charged HiTrap chelating agarose column chromatography and Superdex 200 gel filtration
-
Q-Sepharose column chromatography and HiTrap column chromatography
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration to near homogeneity
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS and Mycobacterium bovis BCG
-
expressed in Escherichia coli pLysS cells
gene mtkasB, expression of His-tagged enzyme in Escherichia coli
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is up-regulated under aerobic conditions
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
drug development
-
the enzyme, along with the other enzyme involved in the FAS-II complex, is a potential target for development of anti-tubercular drugs
drug development
-
the enzyme, along with the other enzyme involved in the FAS-II complex, is a potential target for development of anti-tubercular drugs
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sridharan, S.; Wang, L.; Brown, A.K.; Dover, L.G.; Kremer, L.; Besra, G.S.; Sacchettini, J.C.
X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB)
J. Mol. Biol.
366
469-480
2007
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Borgaro, J.; Chang, A.; MacHutta, C.; Zhang, X.; Tonge, P.
Substrate recognition by beta-ketoacyl-ACP synthases
Biochemistry
50
10678-10686
2011
Escherichia coli
brenda
Kremer, L.; Douglas, J.D.; Baulard, A.R.; Morehouse, C.; Guy, M.R.; Alland, D.; Dover, L.G.; Lakey, J.H.; Jacobs, W.R.; Brennan, P.J.; Minnikin, D.E.; Besra, G.S.
Thiolactomycin and related analogues as novel anti-mycobacterial agents targeting KasA and KasB condensing enzymes in Mycobacterium tuberculosis
J. Biol. Chem.
275
16857-16864
2000
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Schaeffer, M.L.; Agnihotri, G.; Volker, C.; Kallender, H.; Brennan, P.J.; Lonsdale, J.T.
Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB
J. Biol. Chem.
276
47029-47037
2001
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Molle, V.; Brown, A.; Besra, G.; Cozzone, A.; Kremer, L.
The condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation
J. Biol. Chem.
281
30094-30103
2006
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Machutta, C.A.; Bommineni, G.R.; Luckner, S.R.; Kapilashrami, K.; Ruzsicska, B.; Simmerling, C.; Kisker, C.; Tonge, P.J.
Slow onset inhibition of bacterial beta-ketoacyl-acyl carrier protein synthases by thiolactomycin
J. Biol. Chem.
285
6161-6169
2010
Mycobacterium tuberculosis
brenda
Sridharan, S.; Wang, L.; Brown, A.; Dover, L.; Kremer, L.; Besra, G.; Sacchettini, J.
X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB)
J. Mol. Biol.
366
469-480
2007
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Gao, L.Y.; Laval, F.; Lawson, E.H.; Groger, R.K.; Woodruff, A.; Morisaki, J.H.; Cox, J.S.; Daffe, M.; Brown, E.J.
Requirement for kasB in Mycobacterium mycolic acid biosynthesis, cell wall impermeability and intracellular survival implications for therapy
Mol. Microbiol.
49
1547-1563
2003
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Bhatt, A.; Molle, V.; Besra, G.; Jacobs Jr., W.; Kremer, L.
The Mycobacterium tuberculosis FAS-II condensing enzymes Their role in mycolic acid biosynthesis, acid-fastness, pathogenesis and in future drug development
Mol. Microbiol.
64
1442-1454
2007
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda