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Information on EC 2.3.1.285 - (13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase and Organism(s) Papaver somniferum and UniProt Accession I3PLR4

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IUBMB Comments
The enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
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Papaver somniferum
UNIPROT: I3PLR4
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The taxonomic range for the selected organisms is: Papaver somniferum
The expected taxonomic range for this enzyme is: Papaver somniferum
Synonyms
(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase, AT1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:(13S,14R)-1,13-dihydroxy-N-methylcanadine O-acetyltransferase
The enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (13S,14R)-1,13-dihydroxy-N-methylcanadine
(13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine + CoA
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-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0165
(13S,14R)-1,13-dihydroxy-N-methylcanadine
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pH not specified in the publication, temperature not specified in the publication
0.0353
acetyl-CoA
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pH not specified in the publication, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme participates in the biosynthesis of the noscapine. Elucidation of the biosynthetic pathway and the enzymes of the HN1 gene cluster
physiological function
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silencing of the AT1 gene causes a significant reduction in both the relative abundance of corresponding transcript and the accumulation of noscapine in the latex of infected plants compared with empty vecxadtor controls
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AT1_PAPSO
471
0
52494
Swiss-Prot
other Location (Reliability: 2)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
de novo production of noscapine in Saccharomyces cerevisiae, through the reconstruction of a biosynthetic pathway comprising over 30 enzymes from plants, bacteria, mammals, and yeast itself. Optimization of conditions leads to an over 18,000fold improvement from initial noscapine titers to about2.2 mg/l. By feeding modified tyrosine derivatives to the optimized noscapine-producing strain, microbial production of halogenated benzylisoquinoline alkaloids is possible
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dang, T.; Chen, X.; Facchini, P.
Acetylation serves as a protective group in noscapine biosynthesis in opium poppy
Nat. Chem. Biol.
11
104-106
2015
Papaver somniferum
Manually annotated by BRENDA team
Li, Y.; Li, S.; Thodey, K.; Trenchard, I.; Cravens, A.; Smolke, C.
Complete biosynthesis of noscapine and halogenated alkaloids in yeast
Proc. Natl. Acad. Sci. USA
115
E3922-E3931
2018
Papaver somniferum (I3PLR4)
Manually annotated by BRENDA team
Winzer, T.; Gazda, V.; He, Z.; Kaminski, F.; Kern, M.; Larson, T.R.; Li, Y.; Meade, F.; Teodor, R.; Vaistij, F.E.; Walker, C.; Bowser, T.A.; Graham, I.A.
A Papaver somniferum 10-gene cluster for synthesis of the anticancer alkaloid noscapine
Science
336
1704-1708
2012
Papaver somniferum (I3PLR4)
Manually annotated by BRENDA team