Information on EC 2.3.1.265 - phosphatidylinositol dimannoside acyltransferase

for references in articles please use BRENDA:EC2.3.1.265
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The expected taxonomic range for this enzyme is: Mycobacterium

EC NUMBER
COMMENTARY hide
2.3.1.265
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol dimannoside acyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an acyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol = CoA + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
(1)
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an acyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol = CoA + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
(2)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
phosphatidylinositol mannoside biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol acyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
PDB
SCOP
CATH
UNIPROT
ORGANISM
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using 100mM Tris-HCl pH 7.0, 230 mM MgCl2 and 12-16% (w/v) PEG 8000
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography
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TALON Co2+ affinity resin column chromatography, and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS, Rosetta-gami (DE3)pLysS, and C41(DE3)cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D131A
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the mutant shows about 35% activity compared to the wild type enzyme
E149A
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the mutant shows about 75% activity compared to the wild type enzyme
E200A
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the mutant shows less than 3% activity compared to the wild type enzyme
F182W/L197W
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the mutant shows less than 3% activity compared to the wild type enzyme
H126A
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the mutant shows less than 3% activity compared to the wild type enzyme
H284A
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the mutant shows about 50% activity compared to the wild type enzyme
R164A
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the mutant shows about 78% activity compared to the wild type enzyme
E149A
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the mutant shows about 75% activity compared to the wild type enzyme
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E200A
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the mutant shows less than 3% activity compared to the wild type enzyme
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H126A
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the mutant shows less than 3% activity compared to the wild type enzyme
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H284A
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the mutant shows about 50% activity compared to the wild type enzyme
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R164A
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the mutant shows about 78% activity compared to the wild type enzyme
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